EIF4E, 1-217aa, Human, His tag, E Coli | MyBioSource MBS203448 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

EIF4E, 1-217aa, Human, His tag, E Coli

catalog :

MBS203448

quantity :

0.05 mg

price :

375 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203448

products type :

Recombinant Protein

products full name :

EIF4E, 1-217aa, Human, His tag, E Coli

products short name :

EIF4E

products name syn :

CBP; EIF4E1; EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4E

other names :

eukaryotic translation initiation factor 4E isoform 1; Eukaryotic translation initiation factor 4E; eukaryotic translation initiation factor 4E; eIF-4E; eIF-4F 25 kDa subunit; eukaryotic translation initiation factor 4E-like 1; mRNA cap-binding protein; eukaryotic translation initiation factor 4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein

products gene name :

EIF4E

other gene names :

EIF4E; EIF4E; CBP; EIF4F; AUTS19; EIF4E1; EIF4EL1; EIF4EL1; EIF4F; eIF-4E; eIF4E

uniprot entry name :

IF4E_HUMAN

sequence length :

217

sequence :

MGSSHHHHHH SSGLVPRGSH MATVEPETTP TPNPPTTEEE KTESNQEVAN PEHYIKHPLQ NRWALWFFKN DKSKTWQANL RLISKFDTVE DFWALYNHIQ LSSNLMPGCD YSLFKDGIEP MWEDEKNKRG GRWLITLNKQ QRRSDLDRFW LETLLCLIGE SFDDYSDDVC GAVVNVRAKG DKIAIWTTEC ENREAVTHIG RVYKERLGLP PKIVIGYQSH ADTATKSGST TKNRFVV

purity :

> 85% by SDS - PAGE

form :

Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 10% Glycerol, 1mM DTT

concentration :

0.5 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Transcription & Translation

products description :

EIF4E, a member of eukaryotic initiation factor 4 families, modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. This protein recognizes and binds to the 7 methyl GTP cap structure of eukaryotic mRNAs, thereby mediating the initiation of translation. It facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Recombinant human EIF4E protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

products references :

Bendahmane A., et al. (2010) PLoS One. 5(6):e11313. Pelletier J., et al. (2010) J Gen Virol. 91(Pt 5):1224-8.

ncbi gi num :

4503535

ncbi acc num :

NP_001959

ncbi gb acc num :

NM_001968.3

ncbi mol weight :

27.2 kDa(237aa), confirmed by MALDI-TOF

ncbi pathways :

Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway (105970); Alpha6-Beta4 Integrin Signaling Pathway (198807); Antiviral Mechanism By IFN-stimulated Genes Pathway (530760); BDNF Signaling Pathway (712093); Cap-dependent Translation Initiation Pathway (105967); Cytokine Signaling In Immune System Pathway (366171); Deadenylation Of MRNA Pathway (160994); Deadenylation-dependent MRNA Decay Pathway (160993); Eukaryotic Translation Initiation Pathway (105966); GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway (105973)

ncbi summary :

The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of cellular mRNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the mRNA. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

uniprot summary :

EIF4E: a protein of the eukaryotic initiation factor 4E family. Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the EIF4F complex. Protein type: Translation initiation; Translation. Chromosomal Location of Human Ortholog: 4q23. Cellular Component: mRNA cap complex; stress granule; cytoplasm; eukaryotic translation initiation factor 4F complex; cytosol. Molecular Function: protein binding; eukaryotic initiation factor 4G binding; translation initiation factor activity; RNA cap binding. Biological Process: translation; viral reproduction; cytokine and chemokine mediated signaling pathway; positive regulation of mitotic cell cycle; regulation of translation; poly(A) tail shortening; mRNA export from nucleus; cellular protein metabolic process; translational initiation; insulin receptor signaling pathway; gene expression; mRNA catabolic process, deadenylation-dependent decay; G1/S transition of mitotic cell cycle; lung development. Disease: Autism, Susceptibility To, 19

size1 :

0.05 mg

price1 :

375 USD

size2 :

0.25 mg

price2 :

925

more info or order :

MyBioSource product webpage