protein

Aldolase A, 1-364aa, Human, His tag, E. coli

MBS203442

0.1 mg

285 USD

Recombinant Protein

Aldolase A, 1-364aa, Human, His tag, E. coli

Aldolase A

ALDOA; ALDA; GSD12; Fructose bisphosphate aldolase A Aldolase A; Aldolase A fructose bisphosphatase; Aldolase A fructose bisphosphate; Fructose 1 6 bisphosphate triosephosphate lyase; Fructose bisphosphate aldolase; Lung cancer antigen NY Lu 1; MGC10942; MGC17716; MGC17767; Muscle type aldolase

fructose-bisphosphate aldolase A isoform 1; Fructose-bisphosphate aldolase A; fructose-bisphosphate aldolase A; epididymis secretory sperm binding protein Li 87p; fructose-1,6-bisphosphate triosephosphate-lyase; lung cancer antigen NY-LU-1; muscle-type aldolase; aldolase A, fructose-bisphosphate; Lung cancer antigen NY-LU-1; Muscle-type aldolase

ALDOA; ALDOA; ALDA; GSD12; HEL-S-87p; ALDA

ALDOA_HUMAN

364

MGSSHHHHHH SSGLVPRGSH MPYQYPALTP EQKKELSDIA HRIVAPGKGI LAADESTGSI AKRLQSIGTE NTEENRRFYR QLLLTADDRV NPCIGGVILF HETLYQKADD GRPFPQVIKS KGGVVGIKVD KGVVPLAGTN GETTTQGLDG LSERCAQYKK DGADFAKWRC VLKIGEHTPS ALAIMENANV LARYASICQQ NGIVPIVEPE ILPDGDHDLK RCQYVTEKVL AAVYKALSDH HIYLEGTLLK PNMVTPGHAC TQKFSHEEIA MATVTALRRT VPPAVTGITF LSGGQSEEEA SINLNAINKC PLLKPWALTF SYGRALQASA LKAWGGKKEN LKAAQEEYVK RALANSLACQ GKYTPSGQAG AAASESLFVS NHAY

> 95% by SDS - PAGE

Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 100mM NaCl, 10% glycerol

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli. Bioactivity: Specific activity is > 1.5 units/mg, one unit will convert 1.0 umole of fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate per minute at pH 7.5 at 25C. Assay: 1. Prepare a 1.45 ml reaction mixture with the following concentrations: 45 mM Tris pH8.0, 0.95mM fructose 1,6-diphosphate, 0.065 mM beta-nicotinamide adenine dinucleotide, 5 unit alpha-glcerophosphate dehydrogenase/triosephosphate isomerase. 2. Add 50 ul of recombinant Aldolase A protein with 1ug, 2ug in reaction mixture. 3. Record the decrease in A340nm for 5 minutes at 25C.

Metabolism

Fructose bisphosphate aldolase A, also known as Aldolase A is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. It is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Deficiency has been associated with myopathy and hemolytic anemia. Recombinant human Aldolase A, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Pfleiderer G., et al. (1975) Beitr Pathol. 156(3):266-79. Kishi H., et al. (1987) Proc Natl Acad uSA. 84(23):8623-7.

34577110

NP_908930

NM_184041.2

41.5 kDa (384aa), confirmed by MALDI-TOF.

Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); Disease Pathway (530764); Fructose And Mannose Metabolism Pathway (82930); Fructose And Mannose Metabolism Pathway (291); Gluconeogenesis Pathway (106204); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (413342); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (468196)

The protein encoded by this gene, Aldolase A (fructose-bisphosphate aldolase), is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing and alternative promoter usage results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 3 and 10. [provided by RefSeq, Aug 2011]

ALDOA: a glycolytic enzyme that catalyzes D-fructose 1,6-bisphosphate - glycerone phosphate + D-glyceraldehyde 3-phosphate. Three forms of aldolase are found in vertebrates - aldolase A in muscle, aldolase B in liver and aldolase C in brain. Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 4.1.2.13; Carbohydrate Metabolism - fructose and mannose; Lyase; Mitochondrial; Carbohydrate Metabolism - pentose phosphate pathway. Chromosomal Location of Human Ortholog: 16p11.2. Cellular Component: I band; extracellular space; membrane; extracellular region; M band; cytosol; nucleus; actin cytoskeleton. Molecular Function: tubulin binding; identical protein binding; protein binding; cytoskeletal protein binding; fructose-bisphosphate aldolase activity; actin binding. Biological Process: platelet activation; striated muscle contraction; glycolysis; actin filament organization; glucose metabolic process; pathogenesis; gluconeogenesis; protein homotetramerization; muscle maintenance; fructose 1,6-bisphosphate metabolic process; regulation of cell shape; platelet degranulation; ATP biosynthetic process; carbohydrate metabolic process; blood coagulation; fructose metabolic process. Disease: Glycogen Storage Disease Xii

0.1 mg

285 USD

0.5 mg

640