AASDHPPT, 14-309aa, Human, His tag, E Coli | MyBioSource MBS203437 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

AASDHPPT, 14-309aa, Human, His tag, E Coli

catalog :

MBS203437

quantity :

0.1 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203437

products type :

Recombinant Protein

products full name :

AASDHPPT, 14-309aa, Human, His tag, E Coli

products short name :

AASDHPPT

products name syn :

AASD-PPT; CGI-80; LYS2; LYS5; L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase

other names :

L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase; L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase; L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase; 4'-phosphopantetheinyl transferase; LYS5 ortholog; alpha-aminoadipic semialdehyde dehydrogenase-phosphopantetheinyl transferase; aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase; 4'-phosphopantetheinyl transferase; Alpha-aminoadipic semialdehyde dehydrogenase-phosphopantetheinyl transferase; AASD-PPT; LYS5 ortholog

products gene name :

AASDHPPT

other gene names :

AASDHPPT; AASDHPPT; LYS2; LYS5; CGI-80; AASD-PPT; AASD-PPT

uniprot entry name :

ADPPT_HUMAN

sequence length :

309

sequence :

MGSSHHHHHH SSGLVPRGSH MEGVRWAFSC GTWLPSRAEW LLAVRSIQPE EKERIGQFVF ARDAKAAMAG RLMIRKLVAE KLNIPWNHIR LQRTAKGKPV LAKDSSNPYP NFNFNISHQG DYAVLAAEPE LQVGIDIMKT SFPGRGSIPE FFHIMKRKFT NKEWETIRSF KDEWTQLDMF YRNWALKESF IKAIGVGLGF ELQRLEFDLS PLNLDIGQVY KETRLFLDGE EEKEWAFEES KIDEHHFVAV ALRKPDGSRH QDVPSQDDSK PTQRQFTILN FNDLMSSAVP MTPEDPSFWD CFCFTEEIPI RNGTKS

purity :

> 95% by SDS - PAGE

form :

Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 1 mM DTT, 10% Glycerol

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Enzymes & Proteases

products description :

AASDHPPT belongs to the P-Pant transferase superfamily. This protein catalyzes the post-translational modification of target proteins by phosphopantetheine and can transfer the 4'-phosphopantetheine moiety from coenzyme A to a serine residue of a broad range of acceptors, such as the acyl carrier domain of FASN (in vitro). It is detected in heart, skeletal muscle, placenta, testis, brain, pancreas, liver and kidney. Recombinant human AASDHPPT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

products references :

Krupenko SA., et al. (2010) J Biol Chem. 285(3):1627-33. Geraghty MT., et al. (2001) Mol Genet Metab. 72(4):336-42.

ncbi gi num :

20357568

ncbi acc num :

NP_056238

ncbi gb acc num :

NM_015423.2

ncbi mol weight :

36.4 kDa(316aa), confirmed by MALDI-TOF

ncbi pathways :

Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway (906000); Defective BTD Causes Biotidinase Deficiency Pathway (906015); Defective CD320 Causes Methylmalonic Aciduria Pathway (906012); Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway (906001); Defective GIF Causes Intrinsic Factor Deficiency Pathway (906004); Defective HLCS Causes Multiple Carboxylase Deficiency Pathway (906014); Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway (906003); Defective MMAA Causes Methylmalonic Aciduria Type CblA Pathway (906010); Defective MMAB Causes Methylmalonic Aciduria Type CblB Pathway (906009); Defective MMACHC Causes Methylmalonic Aciduria And Homocystinuria Type CblC Pathway (906005)

ncbi summary :

The protein encoded by this gene is similar to Saccharomyces cerevisiae LYS5, which is required for the activation of the alpha-aminoadipate dehydrogenase in the biosynthetic pathway of lysine. Yeast alpha-aminoadipate dehydrogenase converts alpha-biosynthetic-aminoadipate semialdehyde to alpha-aminoadipate. It has been suggested that defects in the human gene result in pipecolic acidemia. [provided by RefSeq, Jul 2008]

uniprot summary :

AASDHPPT: Catalyzes the post-translational modification of target proteins by phosphopantetheine. Can transfer the 4 - phosphopantetheine moiety from coenzyme A to a serine residue of a broad range of acceptors, such as the acyl carrier domain of FASN. Belongs to the P-Pant transferase superfamily. AcpS family. Protein type: EC 2.7.8.-; Amino Acid Metabolism - lysine degradation; Transferase; Amino Acid Metabolism - lysine biosynthesis. Chromosomal Location of Human Ortholog: 11q22. Cellular Component: cytosol. Molecular Function: protein binding; phosphopantetheinyltransferase activity; magnesium ion binding. Biological Process: vitamin metabolic process; macromolecule biosynthetic process; pantothenate metabolic process; water-soluble vitamin metabolic process

size1 :

0.1 mg

price1 :

310 USD

size2 :

0.5 mg

price2 :

720

more info or order :

MyBioSource product webpage