protein

ACOT13, 1-140aa, Human, His tag, E Coli

MBS203428

0.05 mg

375 USD

Recombinant Protein

ACOT13, 1-140aa, Human, His tag, E Coli

ACOT13

THEM2; HT012; PNAS-27; Acyl-coenzyme A thioesterase 13

acyl-coenzyme A thioesterase 13 isoform 1; Acyl-coenzyme A thioesterase 13; acyl-coenzyme A thioesterase 13; hypothalamus protein HT012; thioesterase superfamily member 2; acyl-CoA thioesterase 13; Thioesterase superfamily member 2Acyl-coenzyme A thioesterase 13, N-terminally processed

ACOT13

ACOT13; ACOT13; HT012; THEM2; PNAS-27; THEM2; Acyl-CoA thioesterase 13

ACO13_HUMAN

140

MGSSHHHHHH SSGLVPRGSH MTSMTQSLRE VIKAMTKARN FERVLGKITL VSAAPGKVIC EMKVEEEHTN AIGTLHGGLT ATLVDNISTM ALLCTERGAP GVSVDMNITY MSPAKLGEDI VITAHVLKQG KTLAFTSVDL TNKATGKLIA QGRHTKHLGN

> 95% by SDS - PAGE

Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli

Cytoskeleton

Acyl-coenzyme A thioesterase 13, also known ACOT13, belongs to the thioesterase subfamily of esterase family. Highly expressed in kidney with moderate expression in brain, liver and intestines, ACOT13 contains a hotdog-fold and is thought to co-localize with microtubules, possibly playing a role in cellular proliferation events. Deletion of a portion of the q arm of chromosome 6 is associated with early onset intestinal cancer, suggesting the presence of a cancer susceptibility locus. Recombinant human ACOT13, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Kanno K., et al. (2007) J Biol Chem. 282:30728-30736. Cheng Z., et al. (2006) Biochem Biophys Res Commun. 350:850-853.

8923812

NP_060943

NM_018473.3

17.1 kDa (160aa), confirmed by MALDI-TOF

This gene encodes a member of the thioesterase superfamily. In humans, the protein co-localizes with microtubules and is essential for sustained cell proliferation. The orthologous mouse protein forms a homotetramer and is associated with mitochondria. The mouse protein functions as a medium- and long-chain acyl-CoA thioesterase. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2009]

THEM2: Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates. Can also hydrolyze 3- hydroxyphenylacetyl-CoA and 3,4-dihydrohyphenylacetyl-CoA (in vitro). Belongs to the thioesterase PaaI family. Protein type: EC 3.1.2.-. Chromosomal Location of Human Ortholog: 6p22.3. Cellular Component: mitochondrion; spindle; nucleus; cytosol. Molecular Function: acyl-CoA hydrolase activity. Biological Process: metabolic process; protein homotetramerization

0.05 mg

375 USD

0.25 mg

925