protein

Glutaredoxin-3, 1-335aa, Human, His tag, E Coli

MBS203427

0.1 mg

285 USD

Recombinant Protein

Glutaredoxin-3, 1-335aa, Human, His tag, E Coli

[Glutaredoxin-3]

[Thioredoxin-like protein 2; GLRX3; PICOT; TXNL2; GRX3; glutaredoxin 4; bA500G10.4; GLRX4; GRX4; thioredoxin-like 2; thioredoxin-like 2; glutaredoxin 3,]

[glutaredoxin-3 isoform 1; Glutaredoxin-3; glutaredoxin-3; glutaredoxin 3; PKC-interacting cousin of thioredoxin]

[GLRX3; GLRX3; GRX3; GRX4; GLRX4; PICOT; TXNL2; TXNL3; ; PKCq-interacting protein]

E Coli

335

MGSSHHHHHH SSGLVPRGSH MAAGAAEAAV AAVEEVGSAG QFEELLRLKA KSLLVVHFWA PWAPQCAQMN EVMAELAKEL PQVSFVKLEA EGVPEVSEKY EISSVPTFLF FKNSQKIDRL DGAHAPELTK KVQRHASSGS FLPSANEHLK EDLNLRLKKL THAAPCMLFM KGTPQEPRCG FSKQMVEILH KHNIQFSSFD IFSDEEVRQG LKAYSSWPTY PQLYVSGELI GGLDIIKELE ASEELDTICP KAPKLEERLK VLTNKASVML FMKGNKQEAK CGFSKQILEI LNSTGVEYET FDILEDEEVR QGLKAYSNWP TYPQLYVKGE LVGGLDIVKE LKENGELLPI LRGEN

> 95% by SDS-PAGE

Liquid. 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol, 1mM DTT

0.5 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

SDS-Page

Antigen Species: Human. Tag Information: His-tag

Redox Proteins

Glutaredoxin (GRX), also known as thioltransferase, is member of the thiol-disulfide oxidoreductase family. Glutraredoxin catalyzes the reversible reduction of protein-glutathionyl mixed disulfides to free sulfhydryl groups though a monothiol mechanism. Glutaredoxin-3 (Grx3/PICOT) is an essential protein involved in the regulation of signal transduction, for instance during immune cell activation and development of cardiac hypertrophy, presumably in response to redox signals. And it has been shown to interact with PRKCQ. Recombinant human Glutaredoxin-3, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Witte S., et al. (2000) J Biol Chem 275 (3): 1902-9.; Haunhorst P., et al. (2010) Biochem Biophys Res Commun. 394 (2):372-6;

95113651

NP_006532

NM_006541.4

O76003

39.6kDa (355aa), confirmed by MALDI-TOF

This gene encodes a member of the glutaredoxin family. Glutaredoxins are oxidoreductase enzymes that reduce a variety of substrates using glutathione as a cofactor. The encoded protein binds to and modulates the function of protein kinase C theta. The encoded protein may also inhibit apoptosis and play a role in cellular growth, and the expression of this gene may be a marker for cancer. Pseudogenes of this gene are located on the short arm of chromosomes 6 and 9. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Dec 2010]

Together with BOLA2, acts as a cytosolic iron-sulfur (Fe-S) cluster assembly factor that facilitates [2Fe-2S] cluster insertion into a subset of cytosolic proteins (PubMed:26613676, PubMed:27519415). Acts as a critical negative regulator of cardiac hypertrophy and a positive inotropic regulator (). Required for hemoglobin maturation (PubMed:23615448). Does not possess any thyoredoxin activity since it lacks the conserved motif that is essential for catalytic activity.

0.1 mg

285 USD

0.5 mg

640