MDH1, 1-334aa, Human, His tag, E Coli | MyBioSource MBS203418 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

MDH1, 1-334aa, Human, His tag, E Coli

catalog :

MBS203418

quantity :

0.1 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203418

products type :

Recombinant Protein

products full name :

MDH1, 1-334aa, Human, His tag, E Coli

products short name :

MDH1

products name syn :

MDH-s; MDHA; MOR2; Malate dehydrogenase; cytoplasmic

other names :

malate dehydrogenase, cytoplasmic isoform 2; Malate dehydrogenase, cytoplasmic; malate dehydrogenase, cytoplasmic; cytosolic malate dehydrogenase; diiodophenylpyruvate reductase; epididymis secretory protein Li 32; soluble malate dehydrogenase; malate dehydrogenase 1, NAD (soluble); Cytosolic malate dehydrogenase; Diiodophenylpyruvate reductase (EC:1.1.1.96)

products gene name :

MDH1

other gene names :

MDH1; MDH1; MDHA; MOR2; MDH-s; HEL-S-32; MGC:1375; MDHA

uniprot entry name :

MDHC_HUMAN

sequence length :

334

sequence :

MSEPIRVLVT GAAGQIAYSL LYSIGNGSVF GKDQPIILVL LDITPMMGVL DGVLMELQDC ALPLLKDVIATDKEDVAFKD LDVAILVGSM PRREGMERKD LLKANVKIFK SQGAALDKYA KKSVKVIVVG NPANTNCLTA SKSAPSIPKE NFSCLTRLDH NRAKAQIALK LGVTANDVKN VIIWGNHSST QYPDVNHAKV KLQGKEVGVY EALKDDSWLK GEFVTTVQQR GAAVIKARKL SSAMSAAKAI CDHVRDIWFG TPEGEFVSMG VISDGNSYGV PDDLLYSFPV VIKNKTWKFV EGLPINDFSR EKMDLTAKEL TEEKESAFEF LSSALEHHHH HH

purity :

> 95% by SDS - PAGE

form :

Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 10% Glycerol

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli. Bioactivity: Specific activity is > 100 units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH7.5 at 25C. Assay: 1. Prepare a 1.45 ml assay buffer. The final concentrations are 100 mM Potassium phosphate, 0.13mM beta-nicotinamide adenine dinucleotide, reduced form, 0.25mM oxalacetic acid. 2. Add 50 ul of recombinant MDH1 protein with various concentrations (0.03 ug, 0.06 ug, 0.125 ug) in assay buffer. 3. Mix by inversion and record the decrease at A340nm for approximately 5 minutes.

products categories :

Cancer

products description :

MDH1 is one of the two malate dehydrogenases. Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. In particular, MDH1 is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. This protein also regulates p53-dependent cell-cycle arrest and apoptosis in response to glucose deprivation. Recombinant human MDH1 protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography.

products references :

Youn HD., et al. (2009) Cell Death Differ. 16(5):738-48. Waye MM., et al (2005) J Cell Biochem. 94(4):763-73.

ncbi gi num :

5174539

ncbi acc num :

NP_005908

ncbi gb acc num :

NM_005917.3

ncbi mol weight :

37.4 kDa(342aa), confirmed by MALDI-TOF

ncbi pathways :

Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); Citrate Cycle (TCA Cycle) Pathway (82927); Citrate Cycle (TCA Cycle) Pathway (288); Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway (855811); Citrate Cycle (TCA Cycle, Krebs Cycle) Pathway (468202); Citrate Cycle, Second Carbon Oxidation, 2-oxoglutarate = Oxaloacetate Pathway (413348); Citrate Cycle, Second Carbon Oxidation, 2-oxoglutarate = Oxaloacetate Pathway (468204); Cysteine And Methionine Metabolism Pathway (104488); Cysteine And Methionine Metabolism Pathway (103421)

ncbi summary :

Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Nov 2010]

uniprot summary :

MDH1: Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Nov 2010]. Protein type: EC 1.1.1.96; Oxidoreductase; Carbohydrate Metabolism - citrate (TCA) cycle; EC 1.1.1.37; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - glyoxylate and dicarboxylate. Chromosomal Location of Human Ortholog: 2p13.3. Cellular Component: centrosome; extracellular space; mitochondrion; cytoplasm; cytosol. Molecular Function: malic enzyme activity; L-malate dehydrogenase activity; diiodophenylpyruvate reductase activity; NAD binding. Biological Process: malate metabolic process; oxaloacetate metabolic process; NADH metabolic process; tricarboxylic acid cycle; carbohydrate metabolic process; glucose metabolic process; cellular carbohydrate metabolic process; pathogenesis; gluconeogenesis

size1 :

0.1 mg

price1 :

310 USD

size2 :

0.5 mg

price2 :

720

more info or order :

MyBioSource product webpage