protein

AKR7A2, 1-359aa, Human, His tag, E. coli

MBS203414

0.1 mg

375 USD

Recombinant Protein

AKR7A2, 1-359aa, Human, His tag, E. coli

AKR7A2

Aflatoxin B1 aldehyde reductase member 2; AFAR; AFAR1; AFB1-AR1; AKR7; aldo-keto reductase family 7; member A2 AFB1 AR1; AFB1AR1; aflatoxin aldehyde reductase; aflatoxin beta1 aldehyde reductase; Aiar; aldo keto reductase family 7; aldo keto reductase family 7; member A2; aldoketoreductase 7.

aflatoxin B1 aldehyde reductase member 2; Aflatoxin B1 aldehyde reductase member 2; aflatoxin B1 aldehyde reductase member 2; AFB1 aldehyde reductase 1; AFB1-AR 1; HEL-S-166mP; SSA reductase; aflatoxin beta1 aldehyde reductase; aldoketoreductase 7; epididymis secretory sperm binding protein Li 166mP; succinic semialdehyde reductase; aldo-keto reductase family 7, member A2 (aflatoxin aldehyde reductase); AFB1 aldehyde reductase 1; AFB1-AR 1; Aldoketoreductase 7; Succinic semialdehyde reductase; SSA reductase

AKR7A2

AKR7A2; AKR7A2; AFAR; AKR7; AFAR1; AFB1-AR1; AFAR; AFAR1; AKR7; AFB1-AR 1; SSA reductase

ARK72_HUMAN

359

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSELEM LSAASRVVSR AAVHCALRSP PPEARALAMS RPPPPRVASV LGTMEMGRRM DAPASAAAVR AFLERGHTEL DTAFMYSDGQ SETILGGLGL GLGGGDCRVK IATKANPWDG KSLKPDSVRS QLETSLKRLQ CPQVDLFYLH APDHGTPVEE TLHACQRLHQ EGKFVELGLS NYASWEVAEI CTLCKSNGWI LPTVYQGMYN ATTRQVETEL FPCLRHFGLR FYAYNPLAGG LLTGKYKYED KDGKQPVGRF FGNSWAETYR NRFWKEHHFE AIALVEKALQ AAYGASAPSV TSAALRWMYH HSQLQGAHGD AVILGMSSLE QLEQNLAATE EGPLEPAVVD AFNQAWHLVA HECPNYFR

> 90% by SDS - PAGE

Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 20% glycerol

0.5 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli. Bioactivity: Specific activity: approximately 0.25-0.3 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 micromole NADPH per minute at 25C. Specific activity was expressed as units/mg protein. Assay: 1. Prepare a 1ml reaction mix into a suitable container: The final concentrations are 0.1M sodium phosphate (pH7.0), 10mM DL-glyceraldehyde, 0.3mM NADPH. 2. Add 50ul of recombinant AKR7A2 solution with various concentrations (2.5ug, 5ug, 10ug) in 750ul reaction buffer. 3. Mix by inversion and Incubate at 25C for 2.5 minutes. 4. Add 200ul of 50 mM DL-glyceraldehyde as a substrate and immediately mix by inversion. 5. Record the increase at A340nm for 3 minutes.

Redox Proteins

Aldo-keto reductases, such as AKR7A2, are involved in the detoxification of aldehydes and ketones. This protein can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the nonbinding AFB1 dialcohol. It may be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen. Also, it has been proposed previously to catalyze the NADPH-dependent reduction of succinic semialdehyde (SSA) to gamma-Hydroxybutyrate in human brain. Recombinant AKR7A2 protein was expressed in E.coli and purified by using conventional chromatography techniques.

Bodreddigari S., et al. (2008) Chem Res Toxicol. 21(5):1134-42. Malaspina P., et al. (2009) Hum Genomics. 3(2):106-20.

41327764

NP_003680

NM_003689.3

44.0kDa (398aa), confirmed by MALDI-TOF.

Aflatoxin B1 Metabolism Pathway (198808); Aflatoxin Activation And Detoxification Pathway (1127511); Biological Oxidations Pathway (105698); Defective ACY1 Causes Encephalopathy Pathway (1127638); Disease Pathway (530764); Metabolic Disorders Of Biological Oxidation Enzymes Pathway (1127637); Metabolism Pathway (477135); Metabolism Of Xenobiotics By Cytochrome P450 Pathway (83031); Metabolism Of Xenobiotics By Cytochrome P450 Pathway (425); Metapathway Biotransformation (198837)

The protein encoded by this gene belongs to the aldo/keto reductase (AKR) superfamily and AKR7 family, which are involved in the detoxification of aldehydes and ketones. The AKR7 family consists of 3 genes that are present in a cluster on the p arm of chromosome 1. This protein, thought to be localized in the golgi, catalyzes the NADPH-dependent reduction of succinic semialdehyde to the endogenous neuromodulator, gamma-hydroxybutyrate. It may also function as a detoxication enzyme in the reduction of aflatoxin B1 and 2-carboxybenzaldehyde. [provided by RefSeq, Oct 2011]

AKR7A2: Catalyzes the NADPH-dependent reduction of succinic semialdehyde to gamma-hydroxybutyrate. May have an important role in producing the neuromodulator gamma-hydroxybutyrate (GHB). Has broad substrate specificity. Has NADPH-dependent aldehyde reductase activity towards 2-carboxybenzaldehyde, 2- nitrobenzaldehyde and pyridine-2-aldehyde (in vitro). Can reduce 1,2-naphthoquinone and 9,10-phenanthrenequinone (in vitro). Can reduce the dialdehyde protein-binding form of aflatoxin B1 (AFB1) to the non-binding AFB1 dialcohol. May be involved in protection of liver against the toxic and carcinogenic effects of AFB1, a potent hepatocarcinogen. Belongs to the aldo/keto reductase family. Aldo/keto reductase 2 subfamily. Protein type: EC 1.1.1.n11; Oxidoreductase. Chromosomal Location of Human Ortholog: 1p36.13. Cellular Component: Golgi apparatus; mitochondrion; cytosol. Molecular Function: electron carrier activity; aldehyde reductase activity; phenanthrene-9,10-epoxide hydrolase activity. Biological Process: xenobiotic metabolic process; carbohydrate metabolic process; aldehyde metabolic process

0.1 mg

375 USD

0.5 mg

925