Alix, 1-392aa, Human, His tag, E Coli | MyBioSource MBS203410 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Alix, 1-392aa, Human, His tag, E Coli

catalog :

MBS203410

quantity :

0.1 mg

price :

285 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203410

products type :

Recombinant Protein

products full name :

Alix, 1-392aa, Human, His tag, E Coli

products short name :

Alix

products name syn :

AIP1; PDCD6IP; DRIP4; HP95; PDC6I; Programmed cell death 6 interacting protein ALG 2 interacting protein 1; ALG2 interacting protein X; Apoptosis linked gene 2 interacting protein X; Dopamine receptor interacting protein 4; KIAA1375; MGC17003; PDCD6 interacting protein; Programmed cell death 6 interacting protein.

other names :

programmed cell death 6-interacting protein isoform 1; Programmed cell death 6-interacting protein; programmed cell death 6-interacting protein; ALG-2 interacting protein 1; ALG-2-interacting protein X; PDCD6-interacting protein; apoptosis-linked gene 2-interacting protein X; dopamine receptor interacting protein 4; programmed cell death 6 interacting protein; ALG-2-interacting protein 1; ALG-2-interacting protein X; Hp95

other gene names :

PDCD6IP; PDCD6IP; AIP1; ALIX; HP95; DRIP4; AIP1; ALIX; KIAA1375; PDCD6-interacting protein

uniprot entry name :

PDC6I_HUMAN

sequence length :

868

sequence :

MGSSHHHHHH SSGLVPRGSH MATFISVQLK KTSEVDLAKP LVKFIQQTYP SGGEEQAQYC RAAEELSKLR RAAVGRPLDK HEGALETLLR YYDQICSIEP KFPFSENQIC LTFTWKDAFD KGSLFGGSVK LALASLGYEK SCVLFNCAAL ASQIAAEQNL DNDEGLKIAA KHYQFASGAF LHIKETVLSA LSREPTVDIS PDTVGTLSLI MLAQAQEVFF LKATRDKMKD AIIAKLANQA ADYFGDAFKQ CQYKDTLPKE VFPVLAAKHC IMQANAEYHQ SILAKQQKKF GEEIARLQHA AELIKTVASR YDEYVNVKDF SDKINRALAA AKKDNDFIYH DRVPDLKDLD PIGKATLVKS TPVNVPISQK FTDLFEKMVP VSVQQSLAAY NQRKADLVNR SIAQMREATT LA

purity :

> 95% by SDS-PAGE

form :

Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol 1mM DTT.

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Apoptosis

products description :

Alix, also known as PDCD6IP, is a cytoplasmic protein that interacts with apoptosis-associated proteins (ALG-2 and PDCD6) and with the endocytosis-regulator CIN85. It's involved in concentration and sorting of cargo proteins of the multivesicular body for incorporation into intralumenal vesicles that are generated by invagination and scission from the limiting membrane of the endosome. Overexpression of this protein and endophilins results in cytoplasmic vacuolization which may be partly responsible for the protection against cell death. Recombinant PDCD6IP protein was expressed in E.coli and purified by using conventional chromatography techniques.

products references :

Morita E., et al. (2007) EMBO J. 26(19):4215-27. Katoh K., et al. (2004) Arch Biochem Biophys. 421(1):159-65.

ncbi gi num :

22027538

ncbi acc num :

NP_037506

ncbi gb acc num :

NM_013374.5

ncbi mol weight :

45.8 kDa (412aa), confirmed by MALDI-TOF.

ncbi pathways :

Budding And Maturation Of HIV Virion Pathway (106009); Disease Pathway (530764); Endocytosis Pathway (102279); Endocytosis Pathway (102181); HIV Infection Pathway (105983); HIV Life Cycle Pathway (105984); Late Phase Of HIV Life Cycle Pathway (105995); Uptake And Actions Of Bacterial Toxins Pathway (1127565); Uptake And Function Of Anthrax Toxins Pathway (1127574)

ncbi summary :

This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. Related pseudogenes have been identified on chromosome 15. [provided by RefSeq, Jan 2012]

uniprot summary :

Alix: an adaptor protein that may regulate the function of receptor and cytoskeleton-associated tyrosine kinases. A class E vacuolar protein sorting (VPS) factor involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. Fusion between endosomes and the vacuole will then target the cargo proteins to the vacuolar lumen. In case of infection, the HIV-1 virus directly takes advantage of its function for viral exocytosis and budding, via its interaction with HIV-1 p6 protein. May play a role in the regulation of both apoptosis and cell proliferation. Protein type: Apoptosis; Adaptor/scaffold. Chromosomal Location of Human Ortholog: 3p22.3. Cellular Component: focal adhesion; membrane; cytoplasm; melanosome; microtubule organizing center; immunological synapse; cytosol; vesicle. Molecular Function: protein binding; protein homodimerization activity; proteinase activated receptor binding; calcium-dependent protein binding; SH3 domain binding. Biological Process: protein transport; cell separation during cytokinesis; viral reproduction; apoptosis; viral infectious cycle; mitotic metaphase plate congression; nuclear organization and biogenesis

size1 :

0.1 mg

price1 :

285 USD

size2 :

0.5 mg

price2 :

640

more info or order :

MyBioSource product webpage