APRT, 1-180aa, Human, E Coli | MyBioSource MBS203406 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

APRT, 1-180aa, Human, E Coli

catalog :

MBS203406

quantity :

0.1 mg

price :

285 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203406

products type :

Recombinant Protein

products full name :

APRT, 1-180aa, Human, E Coli

products short name :

APRT

products name syn :

AMP; Adenine phosphoribosyltransferase AMP diphosphorylase; AMP pyrophosphorylase; DKFZp686D13177; MGC125856; MGC125857; MGC129961; Transphosphoribosidase.

other names :

adenine phosphoribosyltransferase isoform a; Adenine phosphoribosyltransferase; adenine phosphoribosyltransferase; AMP diphosphorylase; AMP pyrophosphorylase; transphosphoribosidase; adenine phosphoribosyltransferase

products gene name :

APRT

other gene names :

APRT; APRT; AMP; APRTD; APRT

uniprot entry name :

APT_HUMAN

sequence length :

180

sequence :

MADSELQLVE QRIRSFPDFP TPGVVFRDIS PVLKDPASFR AAIGLLARHL KATHGGRIDY IAGLDSRGFL FGPSLAQELG LGCVLIRKRG KLPGPTLWAS YSLEYGKAEL EIQKDALEPG QRVVVVDDLL ATGGTMNAAC ELLGRLQAEV LECVSLVELT SLKGREKLAP VPFFSLLQYE

purity :

> 90% by SDS - PAGE

form :

Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human

other info2 :

Expression System: E Coli

products categories :

Metabolism

products description :

APRT (adenine phosphoribosyltransferase) is a 180 amino acid protein that localizes to the cytoplasm and belongs to the purine/pyrimidine phosphoribosyltransferase family. Existing as a homodimer, APRT functions to catalyze the formation of inorganic pyrophosphate and AMP from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP), a reaction that is essential for both purine metabolism and AMP biosynthesis. It also produces adenine as a by-product of the polyamine biosynthesis pathway. Recombinant human APRT protein was expressed in E.coli and purified by using conventional chromatography techniques.

products references :

Baranowska-Bosiacka I., et al. (2009) Toxicology. 259(1-2):77-83. Liang L., et al. (2007) Cancer Res. 67(5):1910-7.

ncbi gi num :

4502171

ncbi acc num :

NP_000476

ncbi gb acc num :

NM_000485.2

ncbi mol weight :

19.6 kDa (180aa), confirmed by MALDI-TOF.

ncbi pathways :

Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Nucleotides Pathway (106263); Purine Metabolism Pathway (82944); Purine Metabolism Pathway (106265); Purine Metabolism Pathway (307); Purine Salvage Pathway (106273); Adenine And Adenosine Salvage I Pathway (547494); Superpathway Of Purine Nucleotide Salvage (907942)

ncbi summary :

Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

APRT: Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis. Defects in APRT are the cause of adenine phosphoribosyltransferase deficiency (APRTD); also known as 2,8-dihydroxyadenine urolithiasis. An enzymatic deficiency that can lead to urolithiasis and renal failure. Patients have 2,8-dihydroxyadenine (DHA) urinary stones. Belongs to the purine/pyrimidine phosphoribosyltransferase family. Protein type: Nucleotide Metabolism - purine; EC 2.4.2.7; Transferase. Chromosomal Location of Human Ortholog: 16q24. Cellular Component: nucleoplasm; cytoplasm; cytosol. Molecular Function: adenine binding; adenine phosphoribosyltransferase activity; AMP binding. Biological Process: lactation; grooming behavior; cellular response to insulin stimulus; nucleobase, nucleoside and nucleotide metabolic process; adenine salvage; purine base metabolic process; purine salvage. Disease: Adenine Phosphoribosyltransferase Deficiency

size1 :

0.1 mg

price1 :

285 USD

size2 :

0.5 mg

price2 :

640

more info or order :

MyBioSource product webpage