protein

VPS28, 1-221aa, Human, Recombinant, E Coli

MBS203401

0.1 mg

255 USD

Recombinant Protein

VPS28, 1-221aa, Human, Recombinant, E Coli

VPS28

ESCRT-I complex subunit VPS28; H-Vps28; Vacuolar Protein Sorting 28 acuolar protein sorting 28; Vacuolar protein sorting 28 (yeast); Vacuolar protein sorting 28 homolog.

vacuolar protein sorting-associated protein 28 homolog isoform 1; Vacuolar protein sorting-associated protein 28 homolog; vacuolar protein sorting-associated protein 28 homolog; ESCRT-I complex subunit VPS28; yeast class E protein Vps28p homolog; vacuolar protein sorting 28 homolog (S. cerevisiae); ESCRT-I complex subunit VPS28

VPS28

VPS28; VPS28; H-Vps28

VPS28_HUMAN

221

MFHGIPATPG IGAPGNKPEL YEEVKLYKNA REREKYDNMA ELFAVVKTMQ ALEKAYIKDC VSPSEYTAAC SRLLVQYKAA FRQVQGSEIS SIDEFCRKFR LDCPLAMERI KEDRPITIKD DKGNLNRCIA DVVSLFITVM DKLRLEIRAM DEIQPDLREL METMHRMSHL PPDFEGRQTV SQWLQTLSGM SASDELDDSQ VRQMLFDLES AYNAFNRFLH A

> 95% by SDS - PAGE

Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing 1 mM DTT, 10% Glycerol

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human

Expression System: E Coli. Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method)

ubiquitination

VPS28 is involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. This protein is a subunit of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated proteins. VSP28 binding with the TSG101 may play important roles in budding of Retroviruses, such as HIV-1 and Ebola virus. Recombinant human VPS28 was expressed in E.coli and purified by using conventional chromatography.

Woodman P., et al. (2001) J Biol Chem. 276(15):11735-42. Bieniasz PD., et al. (2003) J Virol. 77(8):4794-804.

7705885

NP_057292

NM_016208.3

25.4 kDa (221aa), confirmed by MALDI-TOF.

Assembly Of The HIV Virion Pathway (106008); Budding And Maturation Of HIV Virion Pathway (106009); Disease Pathway (530764); ESCRT-I Complex Pathway (413473); ESCRT-I Complex Pathway (890624); Endocytosis Pathway (102279); Endocytosis Pathway (102181); Endosomal Sorting Complex Required For Transport (ESCRT) Pathway (205244); HIV Infection Pathway (105983); HIV Life Cycle Pathway (105984)

This gene encodes a protein subunit of the ESCRT-I complex (endosomal complexes required for transport), which functions in the transport and sorting of proteins into subcellular vesicles. This complex can also be hijacked to facilitate the budding of enveloped viruses from the cell membrane. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]

VPS28: Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Belongs to the VPS28 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Vesicle. Chromosomal Location of Human Ortholog: 8q24.3. Cellular Component: late endosome membrane; cytoplasm; plasma membrane; endosome membrane; cytosol; endosome. Molecular Function: protein binding; protein complex binding. Biological Process: ubiquitin-dependent protein catabolic process via the multivesicular body pathway; viral reproduction; viral protein processing; negative regulation of protein ubiquitination; virus assembly; viral infectious cycle; protein targeting to vacuole during ubiquitin-dependent protein catabolic process via the MVB pathway; endosome transport

0.1 mg

255 USD

0.5 mg

535