protein

CDC42, 1- 188aa, Human, T7-tag, Recombinant, E Coli

MBS203396

0.1 mg

255 USD

Recombinant Protein

CDC42, 1- 188aa, Human, T7-tag, Recombinant, E Coli

CDC42

G25K; Cell division cycle 42 isoform 1 CDC42Hs; Cell division cycle 42; Small GTP binding protein CDC42.

cell division control protein 42 homolog isoform 1; Cell division control protein 42 homolog; cell division control protein 42 homolog; G25K GTP-binding protein; GTP binding protein, 25kDa; GTP-binding protein, 25kD; dJ224A6.1.1 (cell division cycle 42 (GTP-binding protein, 25kD)); dJ224A6.1.2 (cell division cycle 42 (GTP-binding protein, 25kD)); growth-regulating protein; small GTP binding protein CDC42; cell division cycle 42; G25K GTP-binding protein

CDC42

CDC42; CDC42; G25K; CDC42Hs

CDC42_HUMAN

191

MASMTGGQQM GRGSHMQTIK CVVVGDGAVG KTCLLISYTT NKFPSEYVPT VFDNYAVTVM IGGEPYTLGL FDTAGQEDYD RLRPLSYPQT DVFLVCFSVV SPSSFENVKE KWVPEITHHC PKTPFLLVGT QIDLRDDPST IEKLAKNKQK PITPETAEKL ARDLKAVKYV ECSALTQKGL KNVFDEAILA ALEPPEPKKS RRC

> 95% by SDS - PAGE

Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol, 2mM EDTA

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: T7 tag

Expression System: E Coli. Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method)

Signal Transduction

Cell division cycle 42 isoform 1, also known as CDC42, is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. Also, this protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Loss of CDC42 function caused an increase in the endocytotic uptake of apical proteins, including apical polarity factors such as Crumbs. Recombinant human CDC42, fused to T7-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Kathryn P., et al. (2008) J Cell Biol. 183(6):1129-1143. Li R., et al. (1997) J Biol Chem. 272(52):32830-5.

4757952

NP_001782

NM_001791.3

22.4 kDa (203aa), confirmed by MALDI-TOF.

AGE/RAGE Pathway (698754); Adaptive Immune System Pathway (366160); Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Androgen Receptor Signaling Pathway (198806); Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Axon Guidance Pathway (105688); BDNF Signaling Pathway (712093); Bacterial Invasion Of Epithelial Cells Pathway (149807)

The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes of this gene have been identified on chromosomes 3, 4, 5, 7, 8 and 20. [provided by RefSeq, Apr 2013]

0.1 mg

255 USD

0.5 mg

535