catalog number :
MBS2033933
products full name :
Polyclonal Antibody to Phospholipase C Beta 2 (PLCb2)
products short name :
[Phospholipase C Beta 2]
products name syn :
[Phosphoinositide phospholipase C-beta-2; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2]
other names :
[1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 isoform b; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2; phospholipase C, beta 2; Phosphoinositide phospholipase C-beta-2; Phospholipase C-beta-2; PLC-beta-2]
products gene name :
[PLCb2]
other gene names :
[Plcb2; Plcb2; AI550384; B230399N12; B230205M18Rik; PLC-beta-2]
purity :
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
form :
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
storage stability :
Storage: Avoid repeated freeze/thaw cycles. Store at 4ºC for frequent use. Aliquot and store at -20ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the
expiration date under appropriate storage condition.
tested application :
WB; IHC; ICC; IP.
app notes :
Western blotting: 0.5-2 ug/mL;1:500-2000. Immunohistochemistry: 5-20 ug/mL;1:50-200. Immunocytochemistry: 5-20 ug/mL;1:50-200. Optimal working dilutions must be determined by end user.
image1 heading :
Western Blot (WB)
image2 heading :
Western Blot (WB)
image3 heading :
Immunohistochemistry (IHC)
other info1 :
Fragment: PLCb2 (Met1~Arg250). Conjugate: No Conjugate
other info2 :
Immunoglobulin Type: IgG. Quality Control: Content: The quality control contains recombinant PLCb2 (Met1~Arg250) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%. Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2073133
ncbi acc num :
NP_001277719.1
ncbi gb acc num :
NM_001290790.1
ncbi pathways :
African Trypanosomiasis Pathway (194387); African Trypanosomiasis Pathway (194323); Alzheimer's Disease Pathway (83294); Alzheimer's Disease Pathway (509); Amoebiasis Pathway (167330); Amoebiasis Pathway (167191); Calcium Signaling Pathway (83247); Calcium Signaling Pathway (459); Carbohydrate Digestion And Absorption Pathway (170722); Carbohydrate Digestion And Absorption Pathway (170654)
uniprot summary :
PLCB2: The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Carbohydrate Metabolism - inositol phosphate; EC 3.1.4.11; Phospholipase. Chromosomal Location of Human Ortholog: 2 E5 2 59.43 cM. Cellular Component: cytoplasm; cytosol; Golgi apparatus; plasma membrane. Molecular Function: calcium ion binding; G-protein beta/gamma-subunit complex binding; hydrolase activity; metal ion binding; phosphoinositide phospholipase C activity; phospholipase C activity; phospholipid binding; phosphoric diester hydrolase activity; signal transducer activity. Biological Process: detection of chemical stimulus involved in sensory perception of bitter taste; lipid catabolic process; lipid metabolic process; sensory perception of bitter taste; signal transduction