protein

VEGF, 205-324aa, Mouse, His-tag, Recombinant, E Coli

MBS203390

0.05 mg

375 USD

Recombinant Protein

VEGF, 205-324aa, Mouse, His-tag, Recombinant, E Coli

VEGF

Vegf-a; Vegf120; Vegf164; Vegf188; Vpf; Vascular endothelial growth factor A isoform 3

vascular endothelial growth factor A isoform 3; Vascular endothelial growth factor A; vascular endothelial growth factor A; vascular permeability factor; vascular endothelial growth factor A; Vascular permeability factor; VPF

VEGF

Vegfa; Vegfa; Vpf; Vegf; Vegf; VEGF-A; VPF

VEGFA_MOUSE

324

MGSSHHHHHH SSGLVPRGSH MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKCDKPR R

> 90% by SDS - PAGE

Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol

0.5 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Mouse. Tag: His-tag

Expression System: E Coli. Bioactivity: Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast. The ED50 for this effect is 0.5-1.5 ng/ml. Assay: 1. Cell line: NIH-3T3 (Mus mosculus/Mouse embryonic fibroblast). 2. Maintenance Condition: RPMI 1640 containing 10% FBS . 3. Assay medium: serum free RPMI 1640 . 4. Cell density: 2 x 10,000 cells/well (96 well plate, final volume 100ul) . 5. Incubation time: 24 hr (after sample treatment) . 6. Concentration range: 1 pg/ml - 1 ug/ml . 7. Detection method : Brdu assay

Growth factors

VEGF-A, also known as vascular endothelial growth factor, belong to the VEGF family that mediate angiogenesis. This protein is produced by diverse cell types, including aortic vascular smooth muscle cells, keratinocytes, macrophages and many tumor cells. VEGF-A has important roles in mammalian vascular development and in diseases involving abnormal growth of blood vessels such as tumor-related angiogenesis. Recombinant VEGF-A protein was expressed in E.coli and purified by using conventional chromatography techniques.

Gerber H., et al. (2002) Nature. 417: 954-958. Shibuya M., et al. (2006) J Biochem Mol Biol. 39:469-478.

160358807

NP_001020428

NM_001025257.3

16.3 kDa (141aa), confirmed by MALDI-TOF.

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This gene product is a member of the PDGF/VEGF growth factor family. It is a mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis, endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Also, alternative translation initiation from non-AUG (CUG) and AUG start sites in some transcript variants, give rise to additional isoforms. The expression of some isoforms derived from AUG start codon is affected by a small upstream open reading frame, which is located within an internal ribosome entry site. [provided by RefSeq, Nov 2013]

0.05 mg

375 USD