NDRG1, 1-394aa, Human, His-tag, Recombinant, E Coli | MyBioSource MBS203387 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

NDRG1, 1-394aa, Human, His-tag, Recombinant, E Coli

catalog :

MBS203387

quantity :

0.1 mg

price :

255 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203387

products type :

Recombinant Protein

products full name :

NDRG1, 1-394aa, Human, His-tag, Recombinant, E Coli

products short name :

NDRG1

products name syn :

CAP43; CMT4D; DRG1; GC4; HMSNL; NMSL; PROXY1; RIT42; RTP; TARG1; TDD5; N-myc downstream regulated 1 TDD5; 42 kDa; anti-GC4; cmt4d; Differentiation related gene1 protein; Drg 1; hmsnl; Human mRNA for RTP complete cds; NDRG1; tdds; N myc downstream regulated gene 1; N myc downstream regulated gene 1 protein; Ndr 1; ndr1; NDRG 1; NDRG1 protein; Protein nickel specific induction protein; Nickel specific induction protein Cap43; nmsl; Nmyc downstream regulated; reduced in tumor; reducin; Nmyc downstream regulated gene1; Nmyc downstream regulated gene1 protein; Protein regulated by oxygen 1; Protein regulated by oxygen1; proxy1; Reducing agents and tunicamycin responsive protein; tunicamycin-responsive protein

other names :

protein NDRG1 isoform 1; Protein NDRG1; protein NDRG1; N-myc downstream-regulated gene 1 protein; differentiation-related gene 1 protein; nickel-specific induction protein Cap43; protein regulated by oxygen-1; reducing agents and tunicamycin-responsive protein; N-myc downstream regulated 1; Differentiation-related gene 1 protein; DRG-1; N-myc downstream-regulated gene 1 protein; Nickel-specific induction protein Cap43; Reducing agents and tunicamycin-responsive protein; RTP; Rit42

products gene name :

NDRG1

other gene names :

NDRG1; NDRG1; GC4; RTP; DRG1; NDR1; NMSL; TDD5; CAP43; CMT4D; DRG-1; HMSNL; RIT42; TARG1; PROXY1; CAP43; DRG1; RTP; DRG-1; RTP

uniprot entry name :

NDRG1_HUMAN

sequence length :

394

sequence :

MSREMQDVDL AEVKPLVEKG ETITGLLQEF DVQEQDIETL HGSVHVTLCG TPKGNRPVIL TYHDIGMNHK TCYNPLFNYE DMQEITQHFA VCHVDAPGQQ DGAASFPAGY MYPSMDQLAE MLPGVLQQFG LKSIIGMGTG AGAYILTRFA LNNPEMVEGL VLINVNPCAE GWMDWAASKI SGWTQALPDM VVSHLFGKEE MQSNVEVVHT YRQHIVNDMN PGNLHLFINA YNSRRDLEIE RPMPGTHTVT LQCPALLVVG DSSPAVDAVV ECNSKLDPTK TTLLKMADCG GLPQISQPAK LAEAFKYFVQ GMGYMPSASM TRLMRSRTAS GSSVTSLDGT RSRSHTSEGT RSRSHTSEGT RSRSHTSEGA HLDITPNSGA AGNSAGPKSM EVSCLEHHHH HH

purity :

> 95% by SDS - PAGE

form :

Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.1 mM PMSF, 10% Glycerol

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli. Bioactivity: The ED50 for this effect is 0.5 - 1.5 ng/ml. Measured in a cell proliferation assay using MCF7 cell. Assay: 1. Cell line: MCF7 (Human breast adenocarcinoma cell) . 2. Maintenance Condition: RPMI 1640 containing 10% FBS . 3. Assay medium: serum free RPMI 1640 . 4. Cell density: 2 x 10,000 cells/well (96 well plate, final volume 100ul). 5. Incubation time : 40 hrs (after sample treatment) . 6. Concentration range: 0.19ng/ml - 50ng/ml . 7. Detection method : Brdu assay .

products categories :

Cancer

products description :

N-myc downstream regulated gene (NDRG)-1 is one of 4 members of the NDRG alpha/beta-hydrolase family. This protein is classified in databases as a tumor suppressor and heavy metal-response protein. Its functions include cell-cycle regulation, cellular differentiation, apoptosis, hypoxia response and metal-ion sensing. Mutation in the NDRG1 gene has been reported to be causative for hereditary motor and sensory neuropathy-Lom (HMSNL), a severe autosomal recessive form of Charcot-Marie-Tooth (CMT) disease. Recombinant human NDRG1, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography.

products references :

Richardson DR., et al. (2008) Biochim Biophys Acta. 1783(10):1981-92. Costa M., et al. (2008) Carcinogenesis. 29(1):2-8.

ncbi gi num :

37655183

ncbi acc num :

NP_006087

ncbi gb acc num :

NM_006096.3

ncbi mol weight :

43.9 kDa (402aa), confirmed by MALDI-TOF.

ncbi pathways :

Direct P53 Effectors Pathway 137939!!HIF-1-alpha Transcription Factor Network Pathway 138045!!Prostate Cancer Pathway 755440!!Validated Targets Of C-MYC Transcriptional Repression Pathway 169353

ncbi summary :

This gene is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein involved in stress responses, hormone responses, cell growth, and differentiation. The encoded protein is necessary for p53-mediated caspase activation and apoptosis. Mutations in this gene are a cause of Charcot-Marie-Tooth disease type 4D, and expression of this gene may be a prognostic indicator for several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

size :

0.1 mg

price :

255 USD

more info or order :

MyBioSource product webpage