Polyclonal Antibody to Calpain 9 (CAPN9) | MyBioSource MBS2033864 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Polyclonal Antibody to Calpain 9 (CAPN9)

catalog :

MBS2033864

quantity :

0.01 mg

price :

110 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot: Sample: Human HCT116 cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Human CAPN9 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody

image 2 :

Western Blot: Sample: Recombinant protein.

image 3 :

DAB staining on fromalin fixed paraffin- embedded stomach tissue)

product information

catalog number :

MBS2033864

products type :

Antibody

products full name :

Polyclonal Antibody to Calpain 9 (CAPN9)

products short name :

[Calpain 9]

products name syn :

[GC36; nCL4; Novel Calpain Large Subunit-4; Digestive tract-specific calpain; New calpain 4]

other names :

[calpain-9 isoform 3; Calpain-9; calpain-9; calpain 9; Digestive tract-specific calpain; New calpain 4; nCL-4; Protein CG36]

products gene name :

[CAPN9]

other gene names :

[CAPN9; CAPN9; GC36; nCL-4; NCL4; nCL-4]

clonality :

Polyclonal

isotype :

IgG

host :

Rabbit

purity :

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

form :

Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

concentration :

1mg/ml

storage stability :

Avoid repeated freeze/thaw cycles. Store at 4ºC for frequent use. Aliquot and store at -20ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP)

app notes :

Western blotting: 0.5-2 ug/mL;1:500-2000. Immunohistochemistry: 5-20 ug/mL;1:50-200. Immunocytochemistry: 5-20 ug/mL;1:50-200. Optimal working dilutions must be determined by end user.

image1 heading :

Western Blot (WB)

image2 heading :

Western Blot (WB)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Immunohistochemistry (IHC)

image4 description :

DAB staining on IHC-P; Samples: Human Stomach Tissue)

image5 heading :

Knockout Validation

image5 description :

Knockout Validation: Lane 1: Wild-type HCT116 cell lysate; Lane 2: CAPN9 knockout HCT116 cell lysate; Predicted MW: 76,79kd Observed MW: 80kd Primary Ab: 1ug/ml Rabbit Anti-Human CAPN9 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047)

other info1 :

Organism Species: Homo sapiens (Human). Source: Polyclonal antibody preparation. Traits: Liquid. Immunogen: Recombinant CAPN9 (Leu42~Ala340) expressed in E.coli

products categories :

Knock-Out Validation Antibody

ncbi gi num :

985567541

ncbi acc num :

NP_001306605.1

ncbi gb acc num :

NM_001319676.1

uniprot acc num :

O14815

ncbi pathways :

Degradation Of The Extracellular Matrix Pathway (1270257); Extracellular Matrix Organization Pathway (1270244); Integrin-mediated Cell Adhesion Pathway (198816)

ncbi summary :

Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large subunit possesses a cysteine protease domain, and both subunits possess calcium-binding domains. Calpains have been implicated in neurodegenerative processes, as their activation can be triggered by calcium influx and oxidative stress. The protein encoded by this gene is expressed predominantly in stomach and small intestine and may have specialized functions in the digestive tract. This gene is thought to be associated with gastric cancer. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

CAPN9: Calcium-regulated non-lysosomal thiol-protease. Belongs to the peptidase C2 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Calcium-binding; EC 3.4.22.-; Protease. Chromosomal Location of Human Ortholog: 1q42.2. Cellular Component: cytoplasm. Molecular Function: calcium ion binding; calcium-dependent cysteine-type endopeptidase activity. Biological Process: digestion; proteolysis

size1 :

0.01 mg

price1 :

110 USD

size2 :

0.02 mg

price2 :

130

size3 :

0.05 mg

price3 :

175

size4 :

0.1 mg

price4 :

225

size5 :

0.2 mg

price5 :

340

size6 :

1 mg

price6 :

800

more info or order :

MyBioSource product webpage