protein

DHFR, 1-187aa, Human, His-tag, Recombinant, E Coli

MBS203372

0.1 mg

255 USD

Recombinant Protein

DHFR, 1-187aa, Human, His-tag, Recombinant, E Coli

DHFR

Dihydrofolate reductase; EC 1.5.1.3

dihydrofolate reductase isoform 1; Dihydrofolate reductase; dihydrofolate reductase; dihydrofolate reductase

DHFR

DHFR; DHFR; DYR; DHFRP1

DYR_HUMAN

187

MGSSHHHHHH SSGLVPRGSH MVGSLNCIVA VSQNMGIGKN GDLPWPPLRN EFRYFQRMTT TSSVEGKQNL VIMGKKTWFS IPEKNRPLKG RINLVLSREL KEPPQGAHFL SRSLDDALKL TEQPELANKV DMVWIVGGSS VYKEAMNHPG HLKLFVTRIM QDFESDTFFP EIDLEKYKLL PEYPGVLSDV QEEKGIKYKF EVYEKND

> 95% by SDS - PAGE

Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.1M Nacl 2mM DTT, and 30% glycerol

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli. Bioactivity: Specific activity is 1.5 - 2.5 units/ml and was obtained by measuring the oxidation of NADPH in absorbance at 340 nm during reaction. One unit will convert 1.0 umole of 7,8 dihydrofolate and beta-NADPH to 5,6,7,8-tetrahydrofolate and beta-NADP per minute at pH 6.5 at 25C. Assay: 1. Prepare a 3.2 ml reaction mixture into a suitable container: The final concentrations are 50 mM potassium phosphate, 0.72 mM DHFA, 0.1mM beta-NADPH, 0.003% (w/v) BSA. 2. Equilibrate to 25C and monitor at A340nm until the value is constant using a spectrophotometer. 3. Add 2.5ug of recombinant DHFR into reaction mixture and mix immediately. 4. Record the increase at A340nm for 2 minutes

Redox Proteins

DHFR, also known as Dihydrofolate reductase, is an enzyme that reduces dihydrofolic acid to tetrahydrofolic acid, using NADPH as electron donor, which can be converted to the kinds of tetrahydrofolate cofactors used in 1-carbon transfer chemistry. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Recombinant Dihydrofolate reductase protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Loveridge EJ., et al. (2009) Biochemistry. 48(25):5922-33. Hu Y., et al. (2009) J Cell Biol. 185(1):87-100.

4503323

NP_000782.1

NM_000791.3

23.6 kDa (207aa), confirmed by MALDI-TOF.

Cell Cycle Pathway 530733!!Cell Cycle, Mitotic Pathway 105765!!Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway 906000!!Defective BTD Causes Biotidinase Deficiency Pathway 906015!!Defective CD320 Causes Methylmalonic Aciduria Pathway 906012!!Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway 906001!!Defective GIF Causes Intrinsic Factor Deficiency Pathway 906004!!Defective HLCS Causes Multiple Carboxylase Deficiency Pathway 906014!!Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway 906003!!Defective MMAA Causes Methylmalonic Aciduria Type CblA Pathway 906010

Dihydrofolate reductase converts dihydrofolate into tetrahydrofolate, a methyl group shuttle required for the de novo synthesis of purines, thymidylic acid, and certain amino acids. While the functional dihydrofolate reductase gene has been mapped to chromosome 5, multiple intronless processed pseudogenes or dihydrofolate reductase-like genes have been identified on separate chromosomes. Dihydrofolate reductase deficiency has been linked to megaloblastic anemia. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2014]

0.1 mg

255 USD