CHMP2A, 1-222aa, Human, His tag, E Coli | MyBioSource MBS203371 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

CHMP2A, 1-222aa, Human, His tag, E Coli

catalog :

MBS203371

quantity :

0.1 mg

price :

375 USD

more info or order :

MyBioSource product webpage

image

image 1 :

product information

catalog number :

MBS203371

products type :

Recombinant Protein

products full name :

CHMP2A, 1-222aa, Human, His tag, E Coli

products short name :

[CHMP2A]

products name syn :

[Chromatin modifying protein 2A; BC-2; BC2; CHMP2; VPS2; VPS2A; Chromatin modifying protein 2A Charged multivesicular body protein 2a Vps2 1; hVps2 1; putative breast adenocarcinoma marker; Putative breast adenocarcinoma marker BC2; Vacuolar protein sorting associated protein 2 1.]

other names :

[charged multivesicular body protein 2a; Charged multivesicular body protein 2a; charged multivesicular body protein 2a; charged multivesicular body protein 2A; Chromatin-modifying protein 2a; CHMP2a; Putative breast adenocarcinoma marker BC-2; Vacuolar protein sorting-associated protein 2-1; Vps2-1; hVps2-1]

products gene name :

[CHMP2A]

other gene names :

[CHMP2A; CHMP2A; BC2; BC-2; VPS2; CHMP2; VPS2A; BC2; CHMP2; CHMP2a; Vps2-1; hVps2-1]

host :

E Coli

sequence length :

222

sequence :

MGSSHHHHHH SSGLVPRGSH MDLLFGRRKT PEELLRQNQR ALNRAMRELD RERQKLETQE KKIIADIKKM AKQGQMDAVR IMAKDLVRTR RYVRKFVLMR ANIQAVSLKI QTLKSNNSMA QAMKGVTKAM GTMNRQLKLP QIQKIMMEFE RQAEIMDMKE EMMNDAIDDA MGDEEDEEES DAVVSQVLDE LGLSLTDELS NLPSTGGSLS VAAGGKKAEA AASALADADA DLEERLKNLR RD

purity :

> 90% by SDS-PAGE

form :

Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 0.1 M NaCl 1mM DTT, and 30% glycerol

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

image1 heading :

SDS-Page

products categories :

Metabolism

products description :

CHMP2A, also known as chromatin modifying protein 2A, belongs to the SNF7 family and it is component of the ESCRT III complex, which is required for multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. Recombinant CHMP2A protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

products references :

Lata S., et al. (2008) Science. 321 (5894):1354-7. ; Agromayor M., et al. (2006) J Biol Chem. 281 (32):23083-91.;

ncbi gi num :

7656922

ncbi acc num :

NP_055268

ncbi gb acc num :

NM_014453.3

uniprot acc num :

O43633

ncbi mol weight :

27.2 kDa (242aa), confirmed by MALDI-TOF.

ncbi pathways :

Budding And Maturation Of HIV Virion Pathway (1269090); Cellular Responses To Stress Pathway (1270414); Clathrin Derived Vesicle Budding Pathway (1269881); Disease Pathway (1268854); ESCRT-III Complex Pathway (413475); ESCRT-III Complex Pathway (890627); Endocytosis Pathway (102279); Endocytosis Pathway (102181); Endosomal Sorting Complex Required For Transport (ESCRT) Pathway (1269895); HIV Infection Pathway (1269057)

ncbi summary :

CHMP2A belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression (Tsang et al., 2006 [PubMed 16730941]).[supplied by OMIM, Mar 2008]

uniprot summary :

Probable core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT-III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis (PubMed:21310966). Together with SPAST, the ESCRT-III complex promotes nuclear envelope sealing and mitotic spindle disassembly during late anaphase (PubMed:26040712). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4.

size1 :

0.1 mg

price1 :

375 USD

size2 :

0.5 mg

price2 :

925

more info or order :

MyBioSource product webpage