catalog number :
MBS203361
products type :
Recombinant Protein
products full name :
VAMP4, 1-115aa, Human, His-tag, Recombinant, E Coli
products short name :
VAMP4
products name syn :
VAMP24; Vesicle-associated membrane protein 4 VAMP 24; VAMP 4; VAMP4 protein; Vesicle associated membrane protein 4; Vesicle associated membrane protein 4; isoform CRA a; Vesicle associated membrane protein 4; isoform CRA b.
other names :
vesicle-associated membrane protein 4 isoform 1; Vesicle-associated membrane protein 4; vesicle-associated membrane protein 4; vesicle-associated membrane protein 4
products gene name :
VAMP4
other gene names :
VAMP4; VAMP4; VAMP-4; VAMP24; VAMP-4
uniprot entry name :
VAMP4_HUMAN
sequence :
MPPKFKRHLN DDDVTGSVKS ERRNLLEDDS DEEEDFFLRG PSGPRFGPRN DKIKHVQNQV DEVIDVMQEN ITKVIERGER LDELQDKSES LSDNATAFSN RSKQLRRQMW WRGCKLEHHH HHH
purity :
> 90% by SDS - PAGE
form :
Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 0.1 M NaCl, and 20% glycerol.
concentration :
0.5 mg/ml (determined by Bradford assay)
storage stability :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
tested application :
SDS-PAGE
other info1 :
Antigen Species: Human. Tag: His-tag
other info2 :
Expression System: E Coli. Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method)
products categories :
Neuroscience
products description :
VAMP4, also known as vesicle-associated membrane protein 4, is a member of the synaptobrevin family involved in docking and/or fusion of vesicles with cell membrane. This protein is enriched in the trans-Golgi network and may play a role in trans-Golgi network-to-endosome transport. VAMP4 is involved in the pathway that functions to remove an inhibitor (probably synaptotagmin-4) of calcium-triggered exocytosis during the maturation of secretory granules. Recombinant human VAMP4 protein, fused to His-tag at C-terminus, was expressed in E.coli and purified by using conventional chromatography.
products references :
Tran TH., et al. (2007) J Cell Sci. 120:1028-41. Steegmaier M., et al. (1999) Mol Biol Cell. 10(6):1957-72.
ncbi gb acc num :
NM_003762.4
ncbi mol weight :
14.5 kDa (123aa), confirmed by MALDI-TOF.
ncbi pathways :
SNARE Interactions In Vesicular Transport Pathway 83057!!SNARE Interactions In Vesicular Transport Pathway 467
ncbi summary :
Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. This protein may play a role in trans-Golgi network-to-endosome transport. [provided by RefSeq, Jul 2008]