catalog number :
MBS203359
products type :
Recombinant Protein
products full name :
Riboflavin kinase, 1-162aa, Human, His-tag, Recombinant, E Coli
products short name :
Riboflavin kinase
products name syn :
RIFK; RP11-422N19.2; RFK; 0610038L10Rik; AF031381; KOI 4; Riboflavin kinase; ATP:riboflavin 5' phosphotransferase; Flavokinase
other names :
riboflavin kinase; Riboflavin kinase; riboflavin kinase; 0610038L10Rik; ATP:riboflavin 5'-phosphotransferase; flavokinase; riboflavin kinase; ATP:riboflavin 5'-phosphotransferase; Flavokinase
other gene names :
RFK; RFK; RIFK
uniprot entry name :
RIFK_HUMAN
sequence :
MGSSHHHHHH SSGLVPRGSH MPRADCIMRH LPYFCRGQVV RGFGRGSKQL GIPTANFPEQ VVDNLPADIS TGIYYGWASV GSGDVHKMVV SIGWNPYYKN TKKSMETHIM HTFKEDFYGE ILNVAIVGYL RPEKNFDSLE SLISAIQGDI EEAKKRLELP EHLKIKEDNF FQVSKSKIMN GH
purity :
> 90% by SDS - PAGE
form :
Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol
concentration :
1.0 mg/ml (determined by Bradford assay)
storage stability :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
tested application :
SDS-PAGE
other info1 :
Antigen Species: Human. Tag: His-tag
other info2 :
Expression System: E Coli
products categories :
Enzymes & Proteases
products description :
Riboflavin kinase, also known as flavokinase, belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. It is an enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN). Recombinant riboflavin kinase was expressed in E.coli and purified by using conventional chromatography techniques.
products references :
Karthikeyan S, et al. (2003) Structure. 11(3):265-73. Yazdanpanah B, et al. (2009) Nature. 460(7259):1159-63.
ncbi gb acc num :
NM_018339.5
ncbi mol weight :
20.5 kDa (182 aa), confirmed by MALDI-TOF.
ncbi pathways :
Defective AMN Causes Hereditary Megaloblastic Anemia 1 Pathway 906000!!Defective BTD Causes Biotidinase Deficiency Pathway 906015!!Defective CD320 Causes Methylmalonic Aciduria Pathway 906012!!Defective CUBN Causes Hereditary Megaloblastic Anemia 1 Pathway 906001!!Defective GIF Causes Intrinsic Factor Deficiency Pathway 906004!!Defective HLCS Causes Multiple Carboxylase Deficiency Pathway 906014!!Defective LMBRD1 Causes Methylmalonic Aciduria And Homocystinuria Type CblF Pathway 906003!!Defective MMAA Causes Methylmalonic Aciduria Type CblA Pathway 906010!!Defective MMAB Causes Methylmalonic Aciduria Type CblB Pathway 906009!!Defective MMACHC Causes Methylmalonic Aciduria And Homocystinuria Type CblC Pathway 906005
ncbi summary :
Riboflavin kinase (RFK; EC 2.7.1.26) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis (Karthikeyan et al., 2003 [PubMed 12623014]).[supplied by OMIM, Nov 2009]