protein

Recombinant Serine/Arginine Rich Splicing Factor 1 (SRSF1)

MBS2033588

0.01 mg

115 USD

Recombinant Protein

Recombinant Serine/Arginine Rich Splicing Factor 1 (SRSF1)

[Serine/Arginine Rich Splicing Factor 1]

[ASF; SF2; SFRS1; Splicing Factor 2; Splicing Factor,Arginine/Serine-Rich 1; Alternate Splicing Factor; Alternative Splicing Factor; pre-mRNA-splicing factor SF2, P33 subunit]

[serine/arginine-rich splicing factor 1; Serine/arginine-rich splicing factor 1; serine/arginine-rich splicing factor 1; Splicing factor, arginine/serine-rich 1]

[SRSF1]

[SRSF1; SRSF1; SFRS1; SFRS1]

SRSF1_BOVIN

E Coli

> 97%

Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

Original Concentration 200 Ug/mL

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8ºC for one month. Aliquot and store at -80ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Positive Control; Immunogen; SDS-PAGE; WB. (May be suitable for use in other assays to be determined by the end user.)

SDS-Page

Organism Species: Homo sapiens (Human). Source: Prokaryotic expression. Residues: Cys16~Arg224. Tags: N-terminal His Tag. Subcellular Location: Nucleus, Cytoplasm. Traits: Freeze-dried powder. Buffer formulation: 100mMNaHCO3, 500mMNaCl, pH8.3, containing 0.01% SKL, 5% Trehalose.

Predicted isoelectric point: 9.9. Predicted Molecular Mass: 27.2kDa. Accurate Molecular Mass: 27kDa as determined by SDS-PAGE reducing conditions. USAGE: Reconstitute in 100mM NaHCO3, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex

115497078

NP_001069862.1

NM_001076394.1

Cleavage Of Growing Transcript In The Termination Region Pathway (1332399); Gene Expression Pathway (1332365); Herpes Simplex Infection Pathway (377872); Herpes Simplex Infection Pathway (377865); Processing Of Capped Intron-Containing Pre-mRNA Pathway (1332401); RNA Polymerase II Transcription Pathway (1332388); RNA Polymerase II Transcription Termination Pathway (1332398); Spliceosome Pathway (125126); Spliceosome Pathway (124832); Transport Of Mature Transcript To Cytoplasm Pathway (1332406)

Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5'-CGAGGCG-3' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway ().

0.01 mg

115 USD

0.05 mg

205

0.1 mg

290

0.2 mg

365

0.5 mg

700

1 mg

1030