protein

Peroxiredoxin 6, 1-224aa, Human, His-tag, Recombinant, E Coli

MBS203357

0.1 mg

310 USD

Recombinant Protein

Peroxiredoxin 6, 1-224aa, Human, His-tag, Recombinant, E Coli

Peroxiredoxin 6

1-Cys; aiPLA2; AOP2; NSGPx; p29; PRX; Peroxiredoxin6; PRDX6; PRDX 6; PRDX-6; AA690119; Prdx6 rs3; 1 Cys; 1 Cys peroxiredoxin; 1 Cys PRX; 1 cysPrx; 24 kDa protein; 9430088D19Rik; Acidic calcium independent phospholipase A2; Antioxidant protein 2; Aop2 rs3; Brp 12; mKIAA0106; Ciliary body glutathione peroxidase; CP 3; EC 1.11.1.15; EC 1.11.1.7; EC 3.1.1; GPx; KIAA0106; Liver 2D page spot 40; Ltw4; Lvtw 4; MGC46173; Non selenium glutathione peroxidase; NSGPx; ORF06; OTTHuMP00000032693; Peroxiredoxin6; PHGPx; Phospholipase A2 lysosomal; PLA2; Prdx5; PRX; Red blood cells page spot 12; Thiol specific antioxidant protein

peroxiredoxin-6; Peroxiredoxin-6; peroxiredoxin-6; 1-Cys PRX; 1-Cys peroxiredoxin; 24 kDa protein; acidic calcium-independent phospholipase A2; antioxidant protein 2; epididymis secretory sperm binding protein Li 128m; liver 2D page spot 40; non-selenium glutathione peroxidase; red blood cells page spot 12; peroxiredoxin 6; 1-Cys peroxiredoxin; 1-Cys PRX; 24 kDa protein; Acidic calcium-independent phospholipase A2 (EC:3.1.1.-); aiPLA2; Antioxidant protein 2; Liver 2D page spot 40; Non-selenium glutathione peroxidase (EC:1.11.1.9); NSGPx; Red blood cells page spot 12

PRDX6; PRDX6; PRX; p29; AOP2; 1-Cys; NSGPx; aiPLA2; HEL-S-128m; AOP2; KIAA0106; 1-Cys PRX; aiPLA2; NSGPx

PRDX6_HUMAN

224

MGSSHHHHHH SSGLVPRGSH MPGGLLLGDV APNFEANTTV GRIRFHDFLG DSWGILFSHP RDFTPVCTTE LGRAAKLAPE FAKRNVKLIA LSIDSVEDHL AWSKDINAYN CEEPTEKLPF PIIDDRNREL AILLGMLDPA EKDEKGMPVT ARVVFVFGPD KKLKLSILYP ATTGRNFDEI LRVVISLQLT AEKRVATPVD WKDGDSVMVL PTIPEEEAKK LFPKGVFTKE LPSGKKYLRY TPQP

> 95% by SDS - PAGE

Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 20% Glycerol

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli. Bioactivity: Specific activity: approximately 95-120 pmole/min/ug. Enzymatic activity was confirmed by measuring the remaining peroxide after incubation of PRDX6 and peroxide for 20 min at room temperature. Specific activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25 C for 1 minute. Assay: 1. Prepare a 50ul reaction mix into a suitable container : . The final concentrations are 1mM DTT, 0.03X PBS, 0.5% glycerol. 2. Add 5 ul of recombinant PRDX6 solution with various concentrations (0.25ug, 0.5ug) in 45 ul reaction buffer. 3. Incubate at 25C for 2 minutes. 4. Add 5ul of 5 mM H2O2 as a substrate and incubate the mixture for 20 min. 5. Add 20ul of 26 % trichloroacetic acid (TCA) to stop the reaction. 6. Add 30ul of Formation solution (10mM Ferrous ammonium sulfate (Fe(II)(NH4)2(SO4)2), 2.5M KSCN) . 7. Record the increase in A475nm.

Redox Proteins

Peroxiredoxin 6, also known as PRDX6, is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell and can reduce H2O2 and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. Recombinant human peroxiredoxin 6 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Davison EJ., et al. (2009). Proteomics. 9(18):4284-97. Wu J., et al. (2009). Chem Res Toxicol. 22(4):668-75.

4758638

NP_004896

NM_004905.2

27.1 kDa (244aa), confirmed by MALDI-TOF.

Cellular Responses To Stress Pathway 645258!!Detoxification Of Reactive Oxygen Species Pathway 1127552!!Metabolic Pathways 132956!!Phenylalanine Metabolism Pathway 82960!!Phenylalanine Metabolism Pathway 327

The protein encoded by this gene is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. [provided by RefSeq, Jul 2008]

0.1 mg

310 USD