PGK1, 1-417aa, Human, His-tag, Recombinant, E Coli | MyBioSource MBS203356 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

PGK1, 1-417aa, Human, His-tag, Recombinant, E Coli

catalog :

MBS203356

quantity :

0.1 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203356

products type :

Recombinant Protein

products full name :

PGK1, 1-417aa, Human, His-tag, Recombinant, E Coli

products short name :

PGK1

products name syn :

MGC117307; MGC142128; MGC8947; MIG10; PGKA; Phosphoglycerate kinase 1; Primer recognition protein 2; PRP 2

other names :

phosphoglycerate kinase 1; Phosphoglycerate kinase 1; phosphoglycerate kinase 1; PRP 2; cell migration-inducing gene 10 protein; epididymis secretory sperm binding protein Li 68p; primer recognition protein 2; phosphoglycerate kinase 1; Cell migration-inducing gene 10 protein; Primer recognition protein 2; PRP 2

products gene name :

PGK1

other gene names :

PGK1; PGK1; PGKA; MIG10; HEL-S-68p; PGKA; PRP 2

uniprot entry name :

PGK1_HUMAN

sequence length :

417

sequence :

MGSSHHHHHH SSGLVPRGSH MSLSNKLTLD KLDVKGKRVV MRVDFNVPMK NNQITNNQRI KAAVPSIKFC LDNGAKSVVL MSHLGRPDGV PMPDKYSLEP VAVELKSLLG KDVLFLKDCV GPEVEKACAN PAAGSVILLE NLRFHVEEEG KGKDASGNKV KAEPAKIEAF RASLSKLGDV YVNDAFGTAH RAHSSMVGVN LPQKAGGFLM KKELNYFAKA LESPERPFLA ILGGAKVADK IQLINNMLDK VNEMIIGGGM AFTFLKVLNN MEIGTSLFDE EGAKIVKDLM SKAEKNGVKI TLPVDFVTAD KFDENAKTGQ ATVASGIPAG WMGLDCGPES SKKYAEAVTR AKQIVWNGPV GVFEWEAFAR GTKALMDEVV KATSRGCITI IGGGDTATCC AKWNTEDKVS HVSTGGGASL ELLEGKVLPG VDALSNI

purity :

> 95% by SDS - PAGE

form :

Liquid. In 20 mM Tris (pH 8.0), containing 10% Glycerol, 1mM DTT.

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli. Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method)

products categories :

Enzymes & Proteases

products description :

Phosphoglycerate kinase 1 (PGK1) is an X-linked enzyme that plays a key role in the glycolytic pathway. The gene encoding the erythrocyte enzyme PGK1 and it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein) as a glyxolytic enzyme role. The PGK1 catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. Recombinant PGK1 protein was expressed in E.coli and purified by using conventional chromatography techniques.

products references :

Svaasand EK., et al. (2007) Muscle Nerve. 36(5):679-84. Valentin C., et al. (1998) Hum Mutat. 12(4):280-7.

ncbi gi num :

4505763

ncbi acc num :

NP_000282

ncbi gb acc num :

NM_000291.3

ncbi mol weight :

46.8 kDa (437aa), confirmed by MALDI-TOF.

ncbi pathways :

Biosynthesis Of Amino Acids Pathway (790012); Biosynthesis Of Amino Acids Pathway (795174); Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); Disease Pathway (530764); Gluconeogenesis Pathway (106204); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (413342); Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway (468196); Glucose Metabolism Pathway (106199); Glycogen Storage Diseases Pathway (1127581)

ncbi summary :

The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome. [provided by RefSeq, Jan 2014]

uniprot summary :

PGK1: an apparent multifunctional protein. A glycolytic enzyme and a polymerase alpha cofactor protein (primer recognition protein). Protein type: Kinase, other; EC 2.7.2.3; Carbohydrate Metabolism - glycolysis and gluconeogenesis. Chromosomal Location of Human Ortholog: Xq13.3. Cellular Component: membrane; cytosol. Molecular Function: protein binding; phosphoglycerate kinase activity; ATP binding. Biological Process: glycolysis; epithelial cell differentiation; carbohydrate metabolic process; glucose metabolic process; pathogenesis; phosphorylation; gluconeogenesis. Disease: Phosphoglycerate Kinase 1 Deficiency

size1 :

0.1 mg

price1 :

310 USD

size2 :

0.5 mg

price2 :

720

more info or order :

MyBioSource product webpage