Midkine, 21-143aa, Human, His-tag, Recombinant, E Coli | MyBioSource MBS203354 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Midkine, 21-143aa, Human, His-tag, Recombinant, E Coli

catalog :

MBS203354

quantity :

0.1 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203354

products type :

Recombinant Protein

products full name :

Midkine, 21-143aa, Human, His-tag, Recombinant, E Coli

products short name :

Midkine

products name syn :

MDK; MK; NEGF2; midkine (neurite growth-promoting factor 2); FLJ27379; Midkine

other names :

midkine isoform a; Midkine; midkine; amphiregulin-associated protein; midgestation and kidney protein; neurite outgrowth-promoting factor 2; retinoic acid inducible factor; midkine (neurite growth-promoting factor 2); Amphiregulin-associated protein; ARAP; Midgestation and kidney protein; Neurite outgrowth-promoting factor 2; Neurite outgrowth-promoting protein

other gene names :

MDK; MDK; MK; ARAP; NEGF2; MK1; NEGF2; MK; ARAP

uniprot entry name :

MK_HUMAN

sequence length :

143

sequence :

MGSSHHHHHH SSGLVPRGSH MVAKKKDKVK KGGPGSECAE WAWGPCTPSS KDCGVGFREG TCGAQTQRIR CRVPCNWKKE FGADCKYKFE NWGACDGGTG TKVRQGTLKK ARYNAQCQET IRVTKPCTPK TKAKAKAKKG KGKD

purity :

> 90% by SDS - PAGE

form :

Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 0.1 M NaCl, 2mM DTT, and 20% Glycerol

concentration :

0.5 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli. Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method)

products categories :

Growth factors

products description :

Midkine, also known as Neurite Growth-promoting Factor 2, is a basic heparin-binding growth factor of low molecular weight, a member of the NEGF family whose founding member is pleiotrophin. This protein exhibits neurite outgrowth-promoting activity and may play a role in nervous system development and/or maintenance. Midkine is pleiotropic, capable of exerting activities such as cell proliferation, cell migration, angiogenesis and fibrinolysis. Recombinant human Midkine protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

products references :

Mashima T., et al. (2009). Oncogene. 28(1):9-19. Jin Z., et al. (2008). Clin Cancer Res. 14(16):5033-42.

ncbi gi num :

4505135

ncbi acc num :

NP_002382

ncbi gb acc num :

NM_002391.4

ncbi mol weight :

15.7 kDa (144aa), confirmed by MALDI-TOF.

ncbi pathways :

Glypican 2 Network Pathway (138035); NOTCH2 Activation And Transmission Of Signal To The Nucleus Pathway (771602); Signal Transduction Pathway (477114); Signaling By NOTCH Pathway (106485); Signaling By NOTCH2 Pathway (576272); Syndecan-4-mediated Signaling Events Pathway (138064)

ncbi summary :

This gene encodes a member of a small family of secreted growth factors that binds heparin and responds to retinoic acid. The encoded protein promotes cell growth, migration, and angiogenesis, in particular during tumorigenesis. This gene has been targeted as a therapeutic for a variety of different disorders. Alternatively spliced transcript variants encoding multiple isoforms have been observed. [provided by RefSeq, Jul 2012]

uniprot summary :

MDK: Developmentally regulated, secreted growth factor homologous to pleiotrophin (PTN), which has heparin binding activity. Binds anaplastic lymphoma kinase (ALK) which induces ALK activation and subsequent phosphorylation of the insulin receptor substrate (IRS1), followed by the activation of mitogen-activated protein kinase (MAPK) and PI3-kinase, and the induction of cell proliferation. Involved in neointima formation after arterial injury, possibly by mediating leukocyte recruitment. Also involved in early fetal adrenal gland development. Belongs to the pleiotrophin family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 11p11.2. Cellular Component: cell projection; cytoplasm; extracellular region. Molecular Function: heparin binding; growth factor activity. Biological Process: response to drug; nervous system development; Notch signaling pathway; cell migration; behavioral fear response; dentate gyrus development; cerebellar granular layer development; short-term memory; adrenal gland development; positive regulation of transcription, DNA-dependent; response to glucocorticoid stimulus; signal transduction; positive regulation of cell division; response to wounding; negative regulation of neuron apoptosis; cerebral cortex development; cell differentiation; defecation; regulation of behavior

size1 :

0.1 mg

price1 :

310 USD

size2 :

0.5 mg

price2 :

720

more info or order :

MyBioSource product webpage