Recombinant Constitutive Androstane Receptor (CAR) | MyBioSource MBS2033523 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Constitutive Androstane Receptor (CAR)

catalog :

MBS2033523

quantity :

0.01 mg

price :

135 USD

more info or order :

MyBioSource product webpage

image

image 1 :

image 2 :

product information

catalog number :

MBS2033523

products type :

Recombinant Protein

products full name :

Recombinant Constitutive Androstane Receptor (CAR)

products short name :

[Constitutive Androstane Receptor]

products name syn :

[NR1I3; NR1-I3; CAR1; MB67; Nuclear Receptor Subfamily 1,Group I,Member 3; Constitutive active response; Constitutive activator of retinoid response; Orphan nuclear receptor MB67]

other names :

[constitutive androstane receptor; Nuclear receptor subfamily 1 group I member 3; nuclear receptor subfamily 1 group I member 3; nuclear receptor subfamily 1 group I member 3; Constitutive activator of retinoid response; Constitutive active response; Constitutive androstane receptor; CAR; Orphan nuclear receptor MB67]

products gene name :

[CAR]

other gene names :

[NR1I3; NR1I3; CAR; CAR1; MB67; CAR; Constitutive active response; CAR]

uniprot entry name :

NR1I3_HUMAN

host :

E. coli

reactivity :

Mouse

purity :

>95%

form :

100mM NaHCO 3 , 500mM NaCl, pH8.3, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300

concentration :

Original Concetration: 200µg/mL

storage stability :

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

Positive Control, Immunogen, SDS-PAGE, WB. (May be suitable for use in other assays to be determined by the end user.)

image1 heading :

Sequence Information

image2 heading :

SDS-Page

other info1 :

Source: Prokaryotic expression. Residues: Met1~Glu247. Tags: N-terminal His-Tag. Tissue Specificity: Liver, Ovary, Uterus. Subcellular Location: Cytoplasm, cytoskeleton, nucleus. Traits: Freeze-dried powder. Predicted isoelectric point: 9.0. Predicted Molecular Mass: 31.8kDa. Accurate Molecular Mass: 32kDa as determined by SDS-PAGE reducing conditions. Usage: Reconstitute in 100mM NaHCO 3 , 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

ncbi gi num :

216409712

ncbi acc num :

BAH02293.1

ncbi pathways :

Drug Induction Of Bile Acid Pathway (698755); Gene Expression Pathway (1269649); Generic Transcription Pathway (1269650); Glucocorticoid Receptor Regulatory Network Pathway (138014); Nuclear Receptor Transcription Pathway (1269652); Nuclear Receptors Pathway (198848); Nuclear Receptors In Lipid Metabolism And Toxicity Pathway (198887)

ncbi summary :

This gene encodes a member of the nuclear receptor superfamily, and is a key regulator of xenobiotic and endobiotic metabolism. The protein binds to DNA as a monomer or a heterodimer with the retinoid X receptor and regulates the transcription of target genes involved in drug metabolism and bilirubin clearance, such as cytochrome P450 family members. Unlike most nuclear receptors, this transcriptional regulator is constitutively active in the absence of ligand but is regulated by both agonists and inverse agonists. Ligand binding results in translocation of this protein to the nucleus, where it activates or represses target gene transcription. These ligands include bilirubin, a variety of foreign compounds, steroid hormones, and prescription drugs. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

NR1I3: Binds and transactivates the retinoic acid response elements that control expression of the retinoic acid receptor beta 2 and alcohol dehydrogenase 3 genes. Transactivates both the phenobarbital responsive element module of the human CYP2B6 gene and the CYP3A4 xenobiotic response element. Interacts with ECT2. Heterodimer of NR1I3 and RXR. Interacts with PSMC4. Directly interacts with DNAJC7. The DNAJC7-NR1I3 complex may also include HSP90. By dexamethasone. Predominantly expressed in liver. Belongs to the nuclear hormone receptor family. NR1 subfamily. 7 isoforms of the human protein are produced by alternative splicing. Protein type: Nuclear receptor; DNA-binding. Chromosomal Location of Human Ortholog: 1q23.3. Cellular Component: cytoplasm; cytoskeleton; cytosol; nucleoplasm; nucleus. Molecular Function: androgen receptor activity; DNA binding; ligand-dependent nuclear receptor activity; thyroid hormone receptor activity; transcription coactivator activity; transcription factor activity; zinc ion binding. Biological Process: androgen receptor signaling pathway; gene expression; intracellular receptor-mediated signaling pathway; negative regulation of transcription, DNA-dependent; osteoblast differentiation; positive regulation of transcription from RNA polymerase II promoter; signal transduction; steroid hormone mediated signaling; transcription initiation from RNA polymerase II promoter

size1 :

0.01 mg

price1 :

135 USD

size2 :

0.05 mg

price2 :

245

size3 :

0.1 mg

price3 :

365

size4 :

0.2 mg

price4 :

450

size5 :

0.5 mg

price5 :

870

size6 :

1 mg

price6 :

1280

more info or order :

MyBioSource product webpage