protein

Recombinant S100 Calcium Binding Protein A12 (S100A12)

MBS2033506

0.01 mg

140 USD

Recombinant Protein

Recombinant S100 Calcium Binding Protein A12 (S100A12)

[S100 Calcium Binding Protein A12]

[CGRP; P6; CAAF1; CAGC; ENRAGE; MRP6; Calgranulin C; Calcium-binding protein in amniotic fluid 1; Extracellular newly identified RAGE-binding; Migration inhibitory factor-related 6]

[S100 calcium binding protein A12; Protein S100-A12; protein S100-A12; S100 calcium binding protein A12; CGRP; Calcium-binding protein in amniotic fluid 1; CAAF1]

[S100A12]

[S100A12; S100A12; p6; CAGC; CGRP; MRP6; CAAF1; MRP-6; ENRAGE; CAAF1; CAGC; EN-RAGE; MRP-6; p6]

S10AC_HUMAN

E. coli

>97%

Original Concentration: 200 ug/mL

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8ºC for one month. Aliquot and store at -80ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Positive Control; Immunogen; SDS-PAGE; WB. (May be suitable for use in other assays to be determined by the end user.)

Sequence Information

SDS-Page

Organism Species: Bos taurus; Bovine (Cattle). Source: Prokaryotic expression. Residues: Thr2~Glu92. Tags: N-terminal His Tag. Subcellular Location: Secreted, Cytoplasm. Traits: Freeze-dried powder. Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01%skl, 5% Trehalose

Predicted isoelectric point: 5.9. Predicted Molecular Mass: 14.2kDa. Accurate Molecular Mass: 14kDa as determined by SDS-PAGE reducing conditions. Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

47124555

AAH70294.1

Activated TLR4 Signalling Pathway (1269236); Advanced Glycosylation Endproduct Receptor Signaling Pathway (1269258); Cytosolic Sensors Of Pathogen-associated DNA Pathway (1269268); DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway (1269276); Immune System Pathway (1269170); Innate Immune System Pathway (1269203); MyD88 Cascade Initiated On Plasma Membrane Pathway (1269207); MyD88 Dependent Cascade Initiated On Endosome Pathway (1269228); MyD88-independent TLR3/TLR4 Cascade Pathway (1269220); MyD88:Mal Cascade Initiated On Plasma Membrane Pathway (1269237)

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities. The protein includes an antimicrobial peptide which has antibacterial activity. [provided by RefSeq, Nov 2014]

S100A12: S100A12 is a calcium-, zinc- and copper-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. Its proinflammatory activity involves recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to receptor for advanced glycation endproducts (AGER). Binding to AGER activates the MAP-kinase and NF-kappa-B signaling pathways leading to production of proinflammatory cytokines and up-regulation of cell adhesion molecules ICAM1 and VCAM1. Acts as a monocyte and mast cell chemoattractant. Can stimulate mast cell degranulation and activation which generates chemokines, histamine and cytokines inducing further leukocyte recruitment to the sites of inflammation. Can inhibit the activity of matrix metalloproteinases; MMP2, MMP3 and MMP9 by chelating Zn(2+) from their active sites. Possesses filariacidal and filariastatic activity. Calcitermin possesses antifungal activity against C.albicans and is also active against E.coli and P.aeruginosa but not L.monocytogenes and S.aureus. Belongs to the S-101 family. Chromosomal Location of Human Ortholog: 1q21. Cellular Component: cytoplasm; cytoskeleton; cytosol; extracellular region; nucleus; plasma membrane. Molecular Function: calcium ion binding; copper ion binding; protein binding; RAGE receptor binding; zinc ion binding. Biological Process: activation of NF-kappaB transcription factor; cytokine secretion; defense response to bacterium; defense response to fungus; inflammatory response; innate immune response; killing of cells of another organism; mast cell activation; monocyte chemotaxis; neutrophil chemotaxis; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of inflammatory response; positive regulation of MAP kinase activity; xenobiotic metabolic process

0.01 mg

140 USD

0.05 mg

255

0.1 mg

390

0.2 mg

480

0.5 mg

935

1 mg

1380