protein

GPI, 1- 558aa, Human, His-tag, Recombinant, E Coli

MBS203349

0.1 mg

310 USD

Recombinant Protein

GPI, 1- 558aa, Human, His-tag, Recombinant, E Coli

GPI

GPI; AMF; GNPI; NLK; PGI; PHI; SA36; EC 5.3.1.9; Neuroleukin; Phosphoglucose isomerase; Phosphohexose isomerase; SA 36; Sperm antigen 36; G6PI; Glucose-6-phosphate isomerase

glucose-6-phosphate isomerase isoform 2; Glucose-6-phosphate isomerase; glucose-6-phosphate isomerase; autocrine motility factor; glucose phosphate isomerase; hexose monophosphate isomerase; hexosephosphate isomerase; neuroleukin; oxoisomerase; phosphoglucose isomerase; phosphohexomutase; phosphohexose isomerase; phosphosaccharomutase; sperm antigen 36; sperm antigen-36; glucose-6-phosphate isomerase; Autocrine motility factor; AMF; Neuroleukin; NLK; Phosphoglucose isomerase; PGI; Phosphohexose isomerase; PHI; Sperm antigen 36; SA-36

GPI

GPI; GPI; AMF; NLK; PGI; PHI; GNPI; SA36; SA-36; GPI; AMF; NLK; PGI; PHI; SA-36

G6PI_HUMAN

558

MGSSHHHHHH SSGLVPRGSH MAALTRDPQF QKLQQWYREH RSELNLRRLF DANKDRFNHF SLTLNTNHGH ILVDYSKNLV TEDVMRMLVD LAKSRGVEAA RERMFNGEKI NYTEGRAVLH VALRNRSNTP ILVDGKDVMP EVNKVLDKMK SFCQRVRSGD WKGYTGKTIT DVINIGIGGS DLGPLMVTEA LKPYSSGGPR VWYVSNIDGT HIAKTLAQLN PESSLFIIAS KTFTTQETIT NAETAKEWFL QAAKDPSAVA KHFVALSTNT TKVKEFGIDP QNMFEFWDWV GGRYSLWSAI GLSIALHVGF DNFEQLLSGA HWMDQHFRTT PLEKNAPVLL ALLGIWYINC FGCETHAMLP YDQYLHRFAA YFQQGDMESN GKYITKSGTR VDHQTGPIVW GEPGTNGQHA FYQLIHQGTK MIPCDFLIPV QTQHPIRKGL HHKILLANFL AQTEALMRGK STEEARKELQ AAGKSPEDLE RLLPHKVFEG NRPTNSIVFT KLTPFMLGAL VAMYEHKIFV QGIIWDINSF DQWGVELGKQ LAKKIEPELD GSAQVTSHDA STNGLINFIK QQREARVQ

> 95% by SDS - PAGE

Liquid. In 20mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli. Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method)

Enzymes & Proteases

Glucose-6-phosphate isomerase, also known as GPI, belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. The protein encoded by this gene is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. Mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons. Recombinant human GPI, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Lin HY., et al. (2009) Biochim Biophys Acta. 1794(2):315-23. Beutler E., et al. (1997) Blood cells Mol. 23:402-409.

18201905

NP_000166

NM_000175.3

65.3 kDa (578aa), confirmed by MALDI-TOF.

Amino Sugar And Nucleotide Sugar Metabolism Pathway (82979); Amino Sugar And Nucleotide Sugar Metabolism Pathway (350); Carbon Metabolism Pathway (814926); Carbon Metabolism Pathway (817567); Disease Pathway (530764); Gluconeogenesis Pathway (106204); Glucose Metabolism Pathway (106199); Glycogen Storage Diseases Pathway (1127581); Glycolysis Pathway (105911); Glycolysis (Embden-Meyerhof Pathway), Glucose = Pyruvate (413340)

This gene encodes a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phophsate and fructose-6-phosphate. Extracellularly, the encoded protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The encoded protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

G6PI: belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. A dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. Functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Protein type: Isomerase; Apoptosis; EC 5.3.1.9; Cytokine; Carbohydrate Metabolism - pentose phosphate pathway; Carbohydrate Metabolism - starch and sucrose; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Carbohydrate Metabolism - glycolysis and gluconeogenesis. Chromosomal Location of Human Ortholog: 19q13.1. Cellular Component: nucleoplasm; extracellular space; neuron projection; membrane; cytoplasm; plasma membrane; cytosol. Molecular Function: monosaccharide binding; glucose-6-phosphate isomerase activity; growth factor activity; cytokine activity; intramolecular transferase activity. Biological Process: aldehyde catabolic process; methylglyoxal biosynthetic process; learning and/or memory; glycolysis; hemostasis; carbohydrate metabolic process; glucose 6-phosphate metabolic process; glucose metabolic process; pathogenesis; negative regulation of neuron apoptosis; angiogenesis; negative regulation of caspase activity; humoral immune response; gluconeogenesis. Disease: Hemolytic Anemia, Nonspherocytic, Due To Glucose Phosphate Isomerase Deficiency

0.1 mg

310 USD

0.5 mg

720