GSTT1, 1-240aa, Human, His-tag, Recombinant, E Coli | MyBioSource MBS203347 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

GSTT1, 1-240aa, Human, His-tag, Recombinant, E Coli

catalog :

MBS203347

quantity :

0.1 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203347

products type :

Recombinant Protein

products full name :

GSTT1, 1-240aa, Human, His-tag, Recombinant, E Coli

products short name :

GSTT1

products name syn :

Glutathione S-transferase theta 1; GSTT1; GST class-theta-1; Glutathione transferase T1-1; Glutathione S-transferase theta 1 EC 2.5.1.18; Glutathione S transferase 5; Glutathione S transferase theta 1; GST class theta 1; Glutathione transferase T1 1; GST 5 5; GST CL1

other names :

Glutathione S-transferase theta 1; Glutathione S-transferase theta-1; glutathione S-transferase theta-1; GST class-theta-1; glutathione transferase T1-1; glutathione S-transferase theta 1; GST class-theta-1; Glutathione transferase T1-1

products gene name :

GSTT1

other gene names :

GSTT1; GSTT1

uniprot entry name :

GSTT1_HUMAN

sequence length :

240

sequence :

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMGL ELYLDLLSQP CRAVYIFAKK NDIPFELRIV DLIKGQHLSD ACAQVNPLKK VPALKDGDFT LTESVAILLY LTRKYKVPDY WYPQDLQARA RVDEYLAWQH TTLRRSCLRA LWHKVMFPVF LGEPVSPQTL AATLAELDVT LQLLEDKFLQ NKAFLTGPHI SLADLVAITE LMHPVGAGCQ VFEGRPKLAT WRQRVEAAVG EDLFQEAHEV ILKAKDFPPA DPTIKQKLMP WVLAMIR

purity :

> 95% by SDS - PAGE

form :

Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol.

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Enzymes & Proteases

products description :

Glutathione S-transferase theta 1(GSTT1) is one of the GSTs' four main classes: alpha, mu, pi, theta. This enzyme is involved in activation and detoxification reactions and catalyzes the conjugation of industrial chemicals, e.g. epoxybutane, ethylene oxides, halomethane with glutathione. The absence of the GSTTI enzyme, might determine the individual risk for development of acquired aplastic anemia and acute myeloid leukemia. Recombinant human GSTT1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

products references :

Faramawy MM., et al. (2009). J Crit Care. 24(3):e7-10. Delpisheh A., et al. (2009). Eur J Obstet Gynecol Reprod Biol. 143(1):38-42.

ncbi gi num :

13937911

ncbi acc num :

AAH07065

ncbi mol weight :

31.5 kDa (277aa), confirmed by MALDI-TOF.

ncbi pathways :

Aflatoxin B1 Metabolism Pathway (198808); Biological Oxidations Pathway (105698); Chemical Carcinogenesis Pathway (673221); Chemical Carcinogenesis Pathway (673237); Defective AHCY Causes Hypermethioninemia With S-adenosylhomocysteine Hydrolase Deficiency (HMAHCHD) Pathway (1127639); Defective GCLC Causes Hemolytic Anemia Due To Gamma-glutamylcysteine Synthetase Deficiency (HAGGSD) Pathway (1127658); Defective GGT1 Causes Glutathionuria (GLUTH) Pathway (1127659); Defective GSS Causes Glutathione Synthetase Deficiency (GSS Deficiency) Pathway (1127660); Defective MAT1A Causes Methionine Adenosyltransferase Deficiency (MATD) Pathway (1127663); Defective OPLAH Causes 5-oxoprolinase Deficiency (OPLAHD) Pathway (1127661)

ncbi summary :

The protein encoded by this gene, glutathione S-transferase (GST) theta 1 (GSTT1), is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human GSTs can be divided into five main classes: alpha, mu, pi, theta, and zeta. The theta class includes GSTT1 and GSTT2. GSTT1 and GSTT2 share 55% amino acid sequence identity and both may play an important role in human carcinogenesis. The GSTT1 and GSTT2 genes have a similar structure, being composed of five exons with identical exon/intron boundaries. This GSTT1 gene is haplotype-specific and is absent from 38% of the population. Alternative splicing of this gene results in multiple transcript variants. Two related pseudogenes, which are also located on chromosome 22, have been identified. [provided by RefSeq, Jun 2014]

uniprot summary :

GSTT1: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Acts on 1,2- epoxy-3-(4-nitrophenoxy)propane, phenethylisothiocyanate 4- nitrobenzyl chloride and 4-nitrophenethyl bromide. Displays glutathione peroxidase activity with cumene hydroperoxide. Belongs to the GST superfamily. Theta family. Protein type: EC 2.5.1.18; Xenobiotic Metabolism - metabolism by cytochrome P450; Transferase; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Other Amino Acids Metabolism - glutathione. Chromosomal Location of Human Ortholog: 22q11.23. Cellular Component: cytosol. Molecular Function: glutathione transferase activity; glutathione peroxidase activity. Biological Process: glutathione metabolic process; xenobiotic metabolic process

size1 :

0.1 mg

price1 :

310 USD

size2 :

0.5 mg

price2 :

720

more info or order :

MyBioSource product webpage