protein

GSTP1, 1-210aa, Human, His-tag, Recombinant, E Coli

MBS203342

0.1 mg

375 USD

Recombinant Protein

GSTP1, 1-210aa, Human, His-tag, Recombinant, E Coli

GSTP1

Glutathione S-transferase P; DFN7; FAEES3; GST3; PI; Glutathione S-transferase P Fatty Acid Ethyl Ester Synthase III; Glutathione S Transferase Pi; GSTP1; Glutathione S-Transferase pi 1

glutathione S-transferase P; Glutathione S-transferase P; glutathione S-transferase P; GST class-pi; GSTP1-1; deafness, X-linked 7; epididymis secretory protein Li 22; fatty acid ethyl ester synthase III; glutathione S-transferase pi 1; GST class-pi; GSTP1-1

GSTP1

GSTP1; GSTP1; PI; DFN7; GST3; GSTP; FAEES3; HEL-S-22; FAEES3; GST3

GSTP1_HUMAN

210

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMPP YTVVYFPVRG RCAALRMLLA DQGQSWKEEV VTVETWQEGS LKASCLYGQL PKFQDGDLTL YQSNTILRHL GRTLGLYGKD QQEAALVDMV NDGVEDLRCK YISLIYTNYE AGKDDYVKAL PGQLKPFETL LSQNQGGKTF IVGDQISFAD YNLLDLLLIH EVLAPGCLDA FPLLSAYVGR LSARPKLKAF LASPEYVNLP INGNGKQ

> 95 % by SDS - PAGE

Liquid. In 20mM Tris-HCl buffer (pH7.0) containing 30% glycerol, 1mM EDTA, 0.1mM PMSF.

0.5 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli. Bioactivity: Specific activity is > 6.5 units/mg, and is defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C. Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method). Assay: 1. Prepare a 1ml reaction mix into a suitable container: The final concentrations are 97 mM potassium phosphate, 0.97 mM EDTA, 2.5 mM glutathione, reduced, 1.0 mM 1-chloro-2,4-dinitrobenzene (CDNB), 3.2% (v/v) ethanol. 2. Equilibrate to 25C and monitor at A340nm until the value is constant using a spectrophotometer. 3. Add 50ul of recombinant GSTP1 protein with various concentrations (1ug, 2ug, 5ug) in 950ul reaction buffer. 4. Mix by inversion and record the increase at A340nm for 5 minutes.

Enzymes & Proteases

GSTP1 is a glutathione S-transferase that belongs to the pi class. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide variety of electrophiles, including reactive products of lipid, protein, carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress. Recombinant GSTP1 protein was expressed in E.coli and purified by using conventional chromatography techniques.

Lee KA, et al. (2001) Blood. 98(12):3483-5. Hayes JD, et al. (1995) Crit Rev Biochem Mol Biol. 30(6):445-600.

4504183

NP_000843

NM_000852.3

27.6 kDa (247 aa), confirmed by MALDI-TOF.

4-hydroxy-2-nonenal Detoxification Pathway (703096); Arachidonate Epoxygenase / Epoxide Hydrolase Pathway (198838); Biological Oxidations Pathway (105698); Cellular Responses To Stress Pathway (645258); Chemical Carcinogenesis Pathway (673221); Chemical Carcinogenesis Pathway (673237); Defective AHCY Causes Hypermethioninemia With S-adenosylhomocysteine Hydrolase Deficiency (HMAHCHD) Pathway (1127639); Defective GCLC Causes Hemolytic Anemia Due To Gamma-glutamylcysteine Synthetase Deficiency (HAGGSD) Pathway (1127658); Defective GGT1 Causes Glutathionuria (GLUTH) Pathway (1127659); Defective GSS Causes Glutathione Synthetase Deficiency (GSS Deficiency) Pathway (1127660)

Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases. [provided by RefSeq, Jul 2008]

GSTP1: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Regulates negatively CDK5 activity via p25/p35 translocation to prevent neurodegeneration. Homodimer. Interacts with CDK5. Belongs to the GST superfamily. Pi family. Protein type: Transferase; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450; Other Amino Acids Metabolism - glutathione; EC 2.5.1.18. Chromosomal Location of Human Ortholog: 11q13. Cellular Component: extracellular space; mitochondrion; cytoplasm; plasma membrane; intracellular; nucleus; cytosol; vesicle. Molecular Function: JUN kinase binding; protein binding; glutathione transferase activity; kinase regulator activity. Biological Process: negative regulation of MAP kinase activity; negative regulation of JNK activity; central nervous system development; negative regulation of MAPKKK cascade; negative regulation of acute inflammatory response; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of tumor necrosis factor production; negative regulation of interleukin-1 beta production; negative regulation of stress-activated MAPK cascade; regulation of stress-activated MAPK cascade; negative regulation of biosynthetic process; response to reactive oxygen species; glutathione metabolic process; negative regulation of fibroblast proliferation; negative regulation of nitric-oxide synthase biosynthetic process; positive regulation of superoxide release; xenobiotic metabolic process; negative regulation of protein kinase activity; negative regulation of apoptosis

0.1 mg

375 USD

0.5 mg

925