Recombinant Beta-Carotene-15,15'-Monooxygenase 1 (bCMO1) | MyBioSource MBS2033403 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Recombinant Beta-Carotene-15,15'-Monooxygenase 1 (bCMO1)

catalog :

MBS2033403

quantity :

0.01 mg

price :

140 USD

more info or order :

MyBioSource product webpage

image

image 1 :

image 2 :

product information

catalog number :

MBS2033403

products type :

Recombinant Protein

products full name :

Recombinant Beta-Carotene-15,15'-Monooxygenase 1 (bCMO1)

products short name :

[Beta-Carotene-15,15'-Monooxygenase 1]

products name syn :

[BCO1; BCDO; BCDO1; BCMO; Beta-carotene dioxygenase 1; Beta-carotene oxygenase 1; Beta,beta-carotene 15,15'-monooxygenase]

other names :

[beta,beta-carotene 15,15'-dioxygenase; Beta,beta-carotene 15,15'-dioxygenase; beta,beta-carotene 15,15'-dioxygenase; beta-carotene oxygenase 1; Beta-carotene dioxygenase 1; Beta-carotene oxygenase 1]

products gene name :

[bCMO1]

other gene names :

[BCO1; BCO1; BCO; BCDO; BCMO; BCDO1; BCMO1]

uniprot entry name :

BCDO1_HUMAN

host :

E Coli

sequence length :

547

sequence :

GRNRK EQLEPVRAKV TGKIPAWLQG TLLRNGPGMH TVGESRYNHWFDGLALLHSF TIRDGEVYYR SKYLRSDTYN TNIEANRIVV SEFGTMAYPDPCKNIFSKAF SYLSHTIPDF TDNCLINIMK CGEDFYATSE TNYIRKINPQTLETLEKVDY RKYVAVNLAT SHPHYDEAGN VLNMGTSIVE KGKTKYVIFKIPATVP

purity :

>99%

form :

20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.

storage stability :

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.

tested application :

SDS-PAGE, WB, ELISA, IP, CoIP, ReporterAssays, Purification, Amine Reactive Labeling. (May be suitable for use in other assays to be determined by the end user.)

image1 heading :

Sequence Information

image2 heading :

SDS-Page

other info1 :

Source: Prokaryotic expression. Residues: Gly6~Pro206. Tags: N-terminal His-Tag. Subcellular Location: Cytosol. Traits: Freeze-dried powder

other info2 :

Predicted isoelectric point: 8.9. Predicted Molecular Mass: 26.6kDa. Accurate Molecular Mass: 27kDa as determined by SDS-PAGE reducing conditions. Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

ncbi gi num :

74027270

ncbi acc num :

NP_059125.2

ncbi gb acc num :

NM_017429.2

ncbi mol weight :

62,637 Da

ncbi pathways :

Metabolic Pathways (132956); Metabolism Pathway (1269956); Metabolism Of Fat-soluble Vitamins Pathway (1339147); Metabolism Of Vitamins And Cofactors Pathway (1270144); Retinoid Metabolism And Transport Pathway (1269624); Retinol Metabolism Pathway (83020); Retinol Metabolism Pathway (406); Signal Transduction Pathway (1269379); Visual Phototransduction Pathway (1269623); Vitamin A And Carotenoid Metabolism Pathway (920901)

ncbi summary :

Vitamin A metabolism is important for vital processes such as vision, embryonic development, cell differentiation, and membrane and skin protection. The protein encoded by this gene is a key enzyme in beta-carotene metabolism to vitamin A. It catalyzes the oxidative cleavage of beta,beta-carotene into two retinal molecules. [provided by RefSeq, Jul 2008]

uniprot summary :

BCMO1: Symmetrically cleaves beta-carotene into two molecules of retinal. The reaction proceeds in three stages, epoxidation of the 15,15'-double bond, hydration of the double bond leading to ring opening, and oxidative cleavage of the diol formed. Defects in BCMO1 are the cause of autosomal dominant hypercarotenemia and vitamin A deficiency (ADHVAD). Vitamin A is essential for normal embryonic development as well as normal physiological functions in children and adults. Hypercarotenemia is characterized by an excess carotene in the serum, but unlike excess vitamin A, carotene is non-toxic. So far, only a few cases of excess vitamin A have been reported. Individuals were thought to be vitamin A deficient due to an impairment in the conversion of carotenoids to retinal in the intestine. Belongs to the carotenoid oxygenase family. Protein type: EC 1.14.99.36; Oxidoreductase; Cofactor and Vitamin Metabolism - retinol. Chromosomal Location of Human Ortholog: 16q23.2. Cellular Component: cytosol. Molecular Function: beta-carotene 15,15'-monooxygenase activity; metal ion binding. Biological Process: fat-soluble vitamin metabolic process; phototransduction, visible light; retinal metabolic process; retinoid metabolic process; retinol metabolic process; vitamin A biosynthetic process; vitamin metabolic process. Disease: Hypercarotenemia And Vitamin A Deficiency, Autosomal Dominant

size1 :

0.01 mg

price1 :

140 USD

size2 :

0.05 mg

price2 :

265

size3 :

0.1 mg

price3 :

410

size4 :

0.2 mg

price4 :

505

size5 :

0.5 mg

price5 :

980

size6 :

1 mg

price6 :

1445

more info or order :

MyBioSource product webpage