protein

GDF-5, 382-501aa, Human, His-tag, Recombinant, E Coli

MBS203335

0.1 mg

310 USD

Recombinant Protein

GDF-5, 382-501aa, Human, His-tag, Recombinant, E Coli

GDF-5

Growth differentiation factor 5; BMP14; CDMP1; LAP4; OS5; SYNS2; Growth differentiation factor 5 Cartilage derived morphogenetic protein 1

growth/differentiation factor 5 preproprotein; Growth/differentiation factor 5; growth/differentiation factor 5; CDMP-1; LPS-associated protein 4; bone morphogenetic protein 14; cartilage-derived morphogenetic protein-1; lipopolysaccharide-associated protein 4; radotermin; growth differentiation factor 5; Bone morphogenetic protein 14; BMP-14; Cartilage-derived morphogenetic protein 1; CDMP-1; Lipopolysaccharide-associated protein 4; LAP-4; LPS-associated protein 4; Radotermin

GDF-5

GDF5; GDF5; OS5; LAP4; BDA1C; BMP14; CDMP1; LAP-4; SYM1B; SYNS2; BMP-14; BMP14; CDMP1; GDF-5; BMP-14; CDMP-1; LAP-4; LPS-associated protein 4

GDF5_HUMAN

501

MGSSHHHHHH SSGLVPRGSH MAPLATRQGK RPSKNLKARC SRKALHVNFK DMGWDDWIIA PLEYEAFHCE GLCEFPLRSH LEPTNHAVIQ TLMNSMDPES TPPTCCVPTR LSPISILFID SANNVVYKQY EDMVVESCGC R

> 95% by SDS - PAGE

Liquid. In 10 mM Sodium citrate pH 3.5, 10% glycerol

0.5 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli. Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method)

Hormones & Cytokines

Growth differentiation factor 5(GDF-5) is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This protein plays a role in chondrogenesis and chondrocyte metabolism, tendon and ligament tissue formation, and bone repair. It also increases the survival of neurones that respond to a neurotransmitter called dopamine, and is a potential therapeutic molecule associated with Parkinson's disease. GDF-5, fused to His-tag at N-terminus, was expressed as insoluble protein aggregate in E.coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Feng G., et al. (2008). Growth Factor. 26(3):132-42. Sullivan AM., et al. (2005). J Anat. 207(3):219-26.

611435007

NP_000548

NM_000557.4

15.8 kDa (141aa), confirmed by MALDI-TOF.

Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!Elastic Fibre Formation Pathway 730310!!Extracellular Matrix Organization Pathway 576262!!Hippo Signaling Pathway 749777!!Hippo Signaling Pathway 750388!!Molecules Associated With Elastic Fibres Pathway 730311!!TGF-beta Signaling Pathway 83064!!TGF-beta Signaling Pathway 475

The protein encoded by this gene is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Mutations in this gene are associated with acromesomelic dysplasia, Hunter-Thompson type; brachydactyly, type C; and chondrodysplasia, Grebe type. These associations confirm that the gene product plays a role in skeletal development. [provided by RefSeq, Jul 2008]

0.1 mg

310 USD