Troponin T, 1-285aa, Human, His-tag, Recombinant, E Coli | MyBioSource MBS203330 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Troponin T, 1-285aa, Human, His-tag, Recombinant, E Coli

catalog :

MBS203330

quantity :

0.1 mg

price :

375 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203330

products type :

Recombinant Protein

products full name :

Troponin T, 1-285aa, Human, His-tag, Recombinant, E Coli

products short name :

Troponin T

products name syn :

Troponin T type 2; cardiac isoform 3; CMH2; CMPD2; cTnT; RCM3; TnTC; Troponin T type 2; cardiac isoform 3 Cardiac muscle troponin T; MGC3889; TNNT 2; TNNT2; Cardiomyopathy hypertrophic 2; Cardiomyopathy dilated 1D (autosomal dominant); CMD1D; Troponin T cardiac muscle; Troponin T type 2 (cardiac); Troponin T type 2 cardiac; Troponin T2 cardiac.

other names :

troponin T, cardiac muscle isoform 3; Troponin T, cardiac muscle; troponin T, cardiac muscle; cardiomyopathy, dilated 1D (autosomal dominant); cardiomyopathy, hypertrophic 2; troponin T2, cardiac; troponin T type 2 (cardiac); Cardiac muscle troponin T; cTnT

other gene names :

TNNT2; TNNT2; CMH2; RCM3; TnTC; cTnT; CMD1D; CMPD2; LVNC6; TnTc; cTnT

uniprot entry name :

TNNT2_HUMAN

sequence length :

285

sequence :

MGSSHHHHHH SSGLVPRGSH MSDIEEVVEE YEEEEQEEAA VEEQEEAAEE DAEAEAETEE TRAEEDEEEE EAKEAEDGPM EESKPKPRSF MPNLVPPKIP DGERVDFDDI HRKRMEKDLN ELQALIEAHF ENRKKEEEEL VSLKDRIERR RAERAEQQRI RNEREKERQN RLAEERARRE EEENRRKAED EARKKKALSN MMHFGGYIQK TERKSGKRQT EREKKKKILA ERRKVLAIDH LNEDQLREKA KELWQSIYNL EAEKFDLQEK FKQQKYEINV LRNRINDNQK VSKTRGKAKV TGRWK

purity :

> 90% by SDS - PAGE

form :

Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

concentration :

0.25 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Cytoskeleton

products description :

Troponin T, also known as TNNT2, is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with restrictive and dilated cardiomyopathy. Recombinant human TNNT2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

products references :

Yu H., et al. (2009), Proteomics. 9(3):504-11. Morita H., et al. (2008). N Engl J Med. 358(18):1899-908.

ncbi gi num :

48255881

ncbi acc num :

NP_001001431

ncbi gb acc num :

NM_001001431.2

ncbi mol weight :

36.4 kDa (305aa), confirmed by MALDI-TOF.

ncbi pathways :

Adrenergic Signaling In Cardiomyocytes Pathway (908257); Adrenergic Signaling In Cardiomyocytes Pathway (909696); Cardiac Progenitor Differentiation Pathway (712094); Cardiac Muscle Contraction Pathway (93344); Cardiac Muscle Contraction Pathway (93992); Dilated Cardiomyopathy Pathway (121494); Dilated Cardiomyopathy Pathway (121285); Hypertrophic Cardiomyopathy (HCM) Pathway (114229); Hypertrophic Cardiomyopathy (HCM) Pathway (106591); Muscle Contraction Pathway (106261)

ncbi summary :

The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq, Jul 2008]

uniprot summary :

TNNT2: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart. Belongs to the troponin T family. 11 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; Motor. Chromosomal Location of Human Ortholog: 1q32. Cellular Component: sarcomere; troponin complex; cytosol; striated muscle thin filament. Molecular Function: troponin C binding; structural constituent of cytoskeleton; troponin I binding; ATPase activity; tropomyosin binding; actin binding. Biological Process: positive regulation of ATPase activity; metabolic process; atrial cardiac muscle morphogenesis; regulation of muscle contraction; ventricular cardiac muscle morphogenesis; sarcomere organization; response to calcium ion; negative regulation of ATPase activity; regulation of heart contraction; muscle filament sliding. Disease: Cardiomyopathy, Familial Restrictive, 3; Cardiomyopathy, Dilated, 1d; Cardiomyopathy, Familial Hypertrophic, 2

size1 :

0.1 mg

price1 :

375 USD

size2 :

0.5 mg

price2 :

925

more info or order :

MyBioSource product webpage