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Carbonic anhydrase 2, 1-260aa, Human, Recombinant, E Coli

MBS203326

0.1 mg

375 USD

MBS203326

Recombinant Protein

Carbonic anhydrase 2, 1-260aa, Human, Recombinant, E Coli

Carbonic anhydrase 2

Carbonic anhydrase 2; CA-II; CAC; CA 2; CA II; CA2; CAI; Car 2; Car2; Carbonate dehydratase II; Carbonic anhydrase B; Carbonic anhydrase C; Carbonic anhydrase II; Carbonic dehydratase.

carbonic anhydrase 2 isoform 1; Carbonic anhydrase 2; carbonic anhydrase 2; carbonate dehydratase II; carbonic anhydrase B; carbonic anhydrase C; carbonic dehydratase; epididymis luminal protein 76; carbonic anhydrase II; Carbonate dehydratase II; Carbonic anhydrase C; CAC; Carbonic anhydrase II; CA-II

CA2; CA2; CAC; CAII; Car2; CA-II; HEL-76; CAC; CA-II

CAH2_HUMAN

260

MSHHWGYGKH NGPEHWHKDF PIAKGERQSP VDIDTHTAKY DPSLKPLSVS YDQATSLRIL NNGHAFNVEF DDSQDKAVLK GGPLDGTYRL IQFHFHWGSL DGQGSEHTVD KKKYAAELHL VHWNTKYGDF GKAVQQPDGL AVLGIFLKVG SAKPGLQKVV DVLDSIKTKG KSADFTNFDP RGLLPESLDY WTYPGSLTTP PLLECVTWIV LKEPISVSSE QVLKFRKLNF NGEGEPEELM VDNWRPAQPL KNRQIKASFK

> 95% by SDS - PAGE

Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 50mM NaCl, 10% glycerol

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human

Expression System: E Coli. Bioactivity: Specific activity is 50-70 nmoles/min/ug and was obtained by measuring the increase in the amount of p-nitropheno by its esterase activity. Specific activity is defined as the amount of p-nitrophenol that 1ug of enzyme can reduce at 25C for 1 minute. Assay: 1. Prepare assay buffer into a suitable container: The final concentrations are 12.5 mM Tris, 75 mM NaCl, pH 7.5. 2. Dilute 4-Nitrophenyl-Acetate(4-NPA) to 2 mM in Assay Buffer. 3. Dilute recombinant CA2 protein with various concentrations (1ug, 2ug, 5ug) in 50 ul assay buffer. 4. Load 50 uL of 2mM 4-NPA into diluted recombinant CA2 solutions, and start the reaction by adding 50 uL of 2 mM Substrate. 5. Read at a wavelength of 405 nm (bottom read) in kinetic mode for 5 minutes.

Enzymes & Proteases

Carbonic anhydrase 2 (CA2) is one of fourteen forms of human alpha carbonic anhydrases. Carbonic anhydrase catalyzes reversible hydration of carbon dioxide. CA2 is a cytosolic enzyme with the highest activity among all known CAs. Mutations in the CA2 gene result in the CA2 deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis and cerebral calcification. Recombinant human CA2 was expressed in E.coli and purified by using conventional chromatography.

Hu PY., et al. (1992). Hum Mutat. 1(4):288-92 . Venta PJ., et al. (1991). Am J Hum Genet. 49(5):1082-90.

4557395

NP_000058

NM_000067.2

29.2 kDa (260aa), confirmed by MALDI-TOF.

Bile Secretion Pathway 193146!!Bile Secretion Pathway 193095!!Collecting Duct Acid Secretion Pathway 147586!!Collecting Duct Acid Secretion Pathway 147560!!Erythrocytes Take Up Carbon Dioxide And Release Oxygen Pathway 645347!!Erythrocytes Take Up Oxygen And Release Carbon Dioxide Pathway 645348!!Gastric Acid Secretion Pathway 154409!!Gastric Acid Secretion Pathway 154383!!Metabolism Pathway 477135!!Nitrogen Metabolism Pathway 83025

The protein encoded by this gene is one of several isozymes of carbonic anhydrase, which catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]

0.1 mg

375 USD