protein

SDF-1, 22-93aa, Human, His-tag, Recombinant, E Coli

MBS203323

0.05 mg

310 USD

Recombinant Protein

SDF-1, 22-93aa, Human, His-tag, Recombinant, E Coli

SDF-1

SDF-1; Stromal cell-derived factor 1; CXCL12; PBSF; hIRH; Stromal cell-derived factor 1 C-X-C motif chemokine 12; OTTHuMP00000019491; 12-O-tetradecanoylphorbol 13-acetate repressed protein 1; AI174028; Chemokine (C-X-C motif) ligand 12; Chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1); Intercrine reduced in hepatomas IRH; Chemokine CXC motif ligand 12; SDF1A; SDF1B; Pre-B cell growth-stimulating factor; SCYB12; SDF 1; Stromal cell-derived factor 1 delta; SDF-1a; SDF-1b; Stromal cell-derived factor 1 gamma; Stromal cell-derived factor 1a; Stromal cell-derived factor-1 alpha; Thymic lymphoma cell-stimulating factor; Tlsf; TLSF-a; TLSF-b; Tlsfa; Tlsfb; TPAR1.

stromal cell-derived factor 1 isoform beta; Stromal cell-derived factor 1; stromal cell-derived factor 1; intercrine reduced in hepatomas; pre-B cell growth-stimulating factor; chemokine (C-X-C motif) ligand 12; C-X-C motif chemokine 12; Intercrine reduced in hepatomas; IRH; hIRH; Pre-B cell growth-stimulating factor; PBSF

SDF-1

CXCL12; CXCL12; IRH; PBSF; SDF1; TLSF; TPAR1; SCYB12; SDF1; SDF1A; SDF1B; SDF-1; hSDF-1; IRH; hIRH; PBSF

SDF1_HUMAN

93

MGSSHHHHHH SSGLVPRGSH MKPVSLSYRC PCRFFESHVA RANVKHLKIL NTPNCALQIV ARLKNNNRQV CIDPKLKWIQ EYLEKALNKR FKM

> 90% by SDS - PAGE

Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

0.5 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human. Tag: His-tag

Expression System: E Coli. Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method)

Hormones & Cytokines

SDF-1 is small cytokine belonging to the chemokine family that is officially designated Chemokine (C-X-C motif) ligand 12 (CXCL12). SDF-1 is strongly chemotactic for lymphocytes and works by its receptor CXCR4. The SDF-1+CXCR4 complex plays a significant role in the creation of metastases of neoplasms and as a response to cytostatic treatment. Identification of this complex may be a useful prognostic factor in the therapy of many types of carcinoma. Recombinant human SDF-1, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Arya M., et al. (2007). Tumour Biol. 28(3):123-31. Kucia M., et al. (2005). Stem Cells. 23(7):879-94.

10834988

NP_000600

NM_000609.6

10.8 kDa (93aa), confirmed by MALDI-TOF.

Axon Guidance Pathway (83065); Axon Guidance Pathway (476); CXCR4-mediated Signaling Events Pathway (137910); Chemokine Receptors Bind Chemokines Pathway (106359); Chemokine Signaling Pathway (99051); Chemokine Signaling Pathway (96864); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytokine-cytokine Receptor Interaction Pathway (83051); Cytokine-cytokine Receptor Interaction Pathway (460); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664)

This antimicrobial gene encodes a stromal cell-derived alpha chemokine member of the intercrine family. The encoded protein functions as the ligand for the G-protein coupled receptor, chemokine (C-X-C motif) receptor 4, and plays a role in many diverse cellular functions, including embryogenesis, immune surveillance, inflammation response, tissue homeostasis, and tumor growth and metastasis. Mutations in this gene are associated with resistance to human immunodeficiency virus type 1 infections. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2014]

CXCL12: Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to another C-X-C chemokine receptor CXCR7, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. SDF-1-beta(3-72) and SDF-1- alpha(3-67) show a reduced chemotactic activity. Binding to cell surface proteoglycans seems to inhibit formation of SDF-1-alpha(3- 67) and thus to preserve activity on local sites. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and CXCR7, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Inhibits CXCR4-mediated infection by T- cell line-adapted HIV-1. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of CXCR7 expressed in various cells. Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation. Monomer or homodimer; in equilibrium. Dimer formation is induced by non acidic pH and the presence of multivalent anions, and by binding to CXCR4 or heparin. Monomeric form is required for full chemotactic activity and resistance to ischemia/reperfusion injury, whereas the dimeric form acts as a partial agonist of CXCR4, stimulating Ca2+ mobilization but with no chemotactic activity and instead acts as a selective antagonist that blocks chemotaxis induced by the monomeric form. Interacts with the N- terminus of CXCR7. Isoform Alpha and isoform Beta are ubiquitously expressed, with highest levels detected in liver, pancreas and spleen. Isoform Gamma is mainly expressed in heart, with weak expression detected in several other tissues. Isoform Delta, isoform Epsilon and isoform Theta have highest expression levels in pancreas, with lower levels detected in heart, kidney, liver and spleen. Belongs to the intercrine alpha (chemokine CxC) family. 6 isoforms of the human protein are produced by alternative splicing. Protein type: Chemokine; Cell development/differentiation; Secreted; Motility/polarity/chemotaxis; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 10q11.1. Cellular Component: extracellular space; extracellular region; external side of plasma membrane. Molecular Function: growth factor activity; chemokine activity; chemokine receptor binding; CXCR chemokine receptor binding; receptor binding. Biological Process: response to peptide hormone stimulus; positive regulation of dopamine secretion; positive regulation of cell adhesion; blood circulation; adult locomotory behavior; neuron migration; motor axon guidance; signal transduction; chemotaxis; induction of positive chemotaxis; germ cell development; T cell proliferation; response to radiation; germ cell migration; cell adhesion; organ regeneration; regulation of actin polymerization and/or depolymerization; response to virus; cellular calcium ion homeostasis; patterning of blood vessels; G-protein coupled receptor protein signaling pathway; response to mechanical stimulus; response to heat; ameboidal cell migration; positive regulation of axon extension involved in axon guidance; response to hypoxia; immune response; positive regulation of endothelial cell proliferation; telencephalon cell migration. Disease: Human Immunodeficiency Virus Type 1, Susceptibility To

0.05 mg

310 USD

0.25 mg

720