catalog number :
MBS203319
products type :
Recombinant Protein
products full name :
SecB, 1-155aa, E Coli, Recombinant, E Coli
products short name :
SecB
products name syn :
Protein export chaperone; SecB; Protein export protein secB
other names :
protein export chaperone; Protein-export protein SecB; protein export chaperone
products gene name :
SecB
other gene names :
secB; secB; ECK3599; JW3584
uniprot entry name :
SECB_ECOLI
sequence :
MSEQNNTEMT FQIQRIYTKD ISFEAPNAPH VFQKDWQPEV KLDLDTASSQ LADDVYEVVL RVTVTASLGE ETAFLCEVQQ GGIFSIAGIE GTQMAHCLGA YCPNILFPYA RECITSMVSR GTFPQLNLAP VNFDALFMNY LQQQAGEGTE EHQDA
purity :
> 95% by SDS - PAGE
form :
Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
concentration :
1 mg/ml (determined by Bradford assay)
storage stability :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
tested application :
SDS-PAGE
other info1 :
Antigen Species: E Coli
other info2 :
Expression System: E Coli
products categories :
Heat Shock Proteins
products description :
SecB, a remarkable chaperone involved in protein export, binds diverse ligands rapidly with high affinity and low specificity. It plays a crucial role during protein export via the general secretory pathway by modulating the partitioning of precursors between folding or aggregation and delivery to the membrane-bound translocation apparatus. SecB has the potential to participate in functions outside of export acting as a general nonspecific chaperone to provide buffering capacity of the nonnative state of proteins in the cytosolic pool. Recombinant E.coli SecB protein was expressed in E.coli and purified by using conventional chromatography.
products references :
Driessen AJ., et al. (2008), Annu Rev Biochem. 77:643-67. Bechtluft P., et al. (2007). Science. 318(5855):1458-61.
ncbi gb acc num :
NC_000913.3
ncbi mol weight :
17.2 kDa (155aa), confirmed by MALDI-TOF.
ncbi pathways :
Bacterial Secretion System Pathway 1124!!Bacterial Secretion System Pathway 447!!Protein Export Pathway 1123!!Protein Export Pathway 446!!Sec (secretion) System Pathway 405292!!Sec (secretion) System Pathway 626823
ncbi summary :
Penultimate Ser residue missing from purified protein could be a proteolytic artifact. [More information is available at EcoGene: EG10937]. SecB is a cytoplasmic protein involved in the Sec secretion pathway and is believed to function in conjunction with SecA/E/G/Y to chaperone proteins in their unfolded state to the inner membrane secretion complex. [More information is available at EcoCyc: EG10937].