AKR1B10, 1-316aa, Human, Recombinant, E Coli | MyBioSource MBS203315 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

AKR1B10, 1-316aa, Human, Recombinant, E Coli

catalog :

MBS203315

quantity :

0.1 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203315

products type :

Recombinant Protein

products full name :

AKR1B10, 1-316aa, Human, Recombinant, E Coli

products short name :

AKR1B10

products name syn :

AKR1B11; AKR1B12; ALDRLn; ARL-1; ARL1; HIS; HIS; Aldo-keto reductase family 1; member B10; AKR1B10; Aldo-keto reductase family 1; member B10 Aldose reductase like; Aldose reductase related protein; ARL 1; hARP; SI reductase; Small intestine reductase.

other names :

aldo-keto reductase family 1 member B10; Aldo-keto reductase family 1 member B10; aldo-keto reductase family 1 member B10; ARP; SI reductase; aldo-keto reductase family 1, member B11 (aldose reductase-like); aldose reductase-like 1; aldose reductase-like peptide; aldose reductase-related protein; hARP; small intestine reductase; aldo-keto reductase family 1, member B10 (aldose reductase); ARL-1; Aldose reductase-like; Aldose reductase-related protein; ARP; hARP; Small intestine reductase; SI reductase

products gene name :

AKR1B10

other gene names :

AKR1B10; AKR1B10; HIS; HSI; ARL1; ARL-1; ALDRLn; AKR1B11; AKR1B12; AKR1B11; ARP; hARP; SI reductase

uniprot entry name :

AK1BA_HUMAN

sequence length :

316

sequence :

MATFVELSTK AKMPIVGLGT WKSPLGKVKE AVKVAIDAGY RHIDCAYVYQ NEHEVGEAIQ EKIQEKAVKR EDLFIVSKLW PTFFERPLVR KAFEKTLKDL KLSYLDVYLI HWPQGFKSGD DLFPKDDKGN AIGGKATFLD AWEAMEELVD EGLVKALGVS NFSHFQIEKL LNKPGLKYKP VTNQVECHPY LTQEKLIQYC HSKGITVTAY SPLGSPDRPW AKPEDPSLLE DPKIKEIAAK HKKTAAQVLI RFHIQRNVIV IPKSVTPARI VENIQVFDFK LSDEEMATIL SFNRNWRACN VLQSSHLEDY PFDAEY

purity :

> 95% by SDS - PAGE

form :

Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol.

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human

other info2 :

Expression System: E Coli. Bioactivity: Specific activity: approximately 0.32 - 0.4 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 micromole NADPH per minute at 25C. Specific activity was expressed as units/mg protein. Assay: 1. Prepare a 1.mll reaction mix into a suitable container : . The final concentrations are 0.1M sodium phosphate (pH7.0), 10mM DL-glyceraldehyde, 0.3mM NADPH. 2. Add 50 ul of recombinant AKR1B10 solution with various concentrations (2.5ug, 5ug, 10ug) in 750 ul reaction buffer. 3. Mix by inversion and Incubate at 25C for 2.5 minutes. 4. Add 200ul of 50 mM DL-glyceraldehyde as a substrate and immediately mix by inversion. 5. Record the decrease at A340nm for 3 minutes.

products categories :

Redox Proteins

products description :

AKR1B10, also known as Aldo-keto reductase family 1, member B10, AKR1B10 is a monomeric protein that efficiently catalyzes the reduction of aromatic and aliphatic aldehydes and ketones. AKR1B10 is ubiquitously expressed in many human tissues but is highly expressed in small intestine, colon and adrenal gland. This protein is pathogenically involved in diabetic complications and has been reported that AKR1B10 is overexpressed in human tumors, such as liver, breast, and lung cancer, and may play a critical role in the development and progression of cancer. Recombinant human AKR1B10 protein was expressed in E.coli and purified by using conventional chromatography.

products references :

Yabe D., et al. (1997) J Biol Chem. 272(29):18232-9. Vorum H., et al. (1998) Biochim Biophys Acta. 1386(1):121-31.

ncbi gi num :

223468663

ncbi acc num :

NP_064695

ncbi gb acc num :

NM_020299.4

ncbi mol weight :

36 kDa (316aa), confirmed by MALDI-TOF.

ncbi pathways :

Disease Pathway (530764); Diseases Associated With Visual Transduction Pathway (771581); Fructose And Mannose Metabolism Pathway (82930); Fructose And Mannose Metabolism Pathway (291); Galactose Metabolism Pathway (82931); Galactose Metabolism Pathway (292); Glycerolipid Metabolism Pathway (82986); Glycerolipid Metabolism Pathway (361); Metabolic Pathways (132956); Pentose And Glucuronate Interconversions Pathway (82929)

ncbi summary :

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member can efficiently reduce aliphatic and aromatic aldehydes, and it is less active on hexoses. It is highly expressed in adrenal gland, small intestine, and colon, and may play an important role in liver carcinogenesis. [provided by RefSeq, Jul 2008]

uniprot summary :

AKR1B10: Acts as all-trans-retinaldehyde reductase. Can efficiently reduce aliphatic and aromatic aldehydes, and is less active on hexoses (in vitro). May be responsible for detoxification of reactive aldehydes in the digested food before the nutrients are passed on to other organs. Belongs to the aldo/keto reductase family. Protein type: Carbohydrate Metabolism - butanoate; Carbohydrate Metabolism - fructose and mannose; Lipid Metabolism - linoleic acid; Oxidoreductase; EC 1.1.1.-; EC 1.1.1.21. Chromosomal Location of Human Ortholog: 7q33. Cellular Component: lysosome; cytosol. Molecular Function: protein binding; aldo-keto reductase activity; indanol dehydrogenase activity; geranylgeranyl reductase activity; retinal dehydrogenase activity. Biological Process: steroid metabolic process; phototransduction, visible light; digestion; aldehyde metabolic process; farnesol catabolic process; retinoid metabolic process

size1 :

0.1 mg

price1 :

310 USD

size2 :

0.5 mg

price2 :

720

more info or order :

MyBioSource product webpage