catalog number :
MBS203300
products type :
Recombinant Protein
products full name :
Protein A, 37-469aa, S.aureus, Recombinant, E Coli
products short name :
protein A
products name syn :
Immunoglobulin G binding protein A; Immunoglobulin G binding protein A Staphylococcal protein A; SPA
other names :
immunoglobulin G binding protein A; Immunoglobulin G binding protein A; Immunoglobulin G binding protein A
uniprot entry name :
B3GGB2_STAAU
sequence :
MAQHDEAQQN AFYQVLNMPN LNADQRNGFI QSLKDDPSQS ANVLGEAQKL NDSQAPKADA QQNNFNKDQQ SAFYEILNMP NLNEAQRNGF IQSLKDDPSQ STNVLGEAKK LNESQAPKAD NNFNKEQQNA FYEILNMPNL NEEQRNGFIQ SLKDDPSQSA NLLSEAKKLN ESQAPKADNK FNKEQQNAFY EILHLPNLNE EQRNGFIQSL KDDPSQSANL LAEAKKLNDA QAPKADNKFN KEQQNAFYEI LHLPNLTEEQ RNGFIQSLKD DPSVSKEILA EAKKLNDAQA PKEEDNNKPG KEDNNKPGKE DNNKPGKEDG NKPGKEDNKK PGKEDNKKPG KEDNKKPGKE DGNKPGKEDN KKPGKEDGNG VHVVKPGDTV NDIAKANGTT ADKIAADNKL ADKNMIKPGQ ELVVDKKQPA NHADANKAQA LPET
purity :
> 90% by SDS - PAGE
form :
Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol,
concentration :
1 mg/ml (determined by BCA assay)
storage stability :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
tested application :
SDS-PAGE
other info1 :
Antigen Species: S.aureus
other info2 :
Expression System: E Coli. Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method)
products categories :
Immunology
products description :
Protein A is a 40-60 kDa surface protein originally found in the cell wall of the bacteria Staphylococcus aureus. It binds with high affinity to human IgG1 and IgG2 as well as mouse IgG2a and IgG2b. Protein A binds with moderate affinity to human IgM, IgA and IgE as well as to mouse IgG3 and IgG1. Due to its affinity for the Fc region of many mammalian immunoglobulins, protein A is considered a universal reagent in biochemistry and immunology. Recombinant Staphylococcus aureus Protein A was expressed in E.coli and purified by using conventional chromatography.
products references :
Guirnalda P., et al. (2007) Int J Parasitol. 37(12):1391-9. Volpers C., et al. (2003) J Virol. 77(3):2093-104.
ncbi mol weight :
48.1 kDa (434aa), confirmed by MALDI-TOF.