catalog number :
MBS2032984
products type :
Recombinant Protein
products full name :
Recombinant Wilms Tumor 1 Associated Protein (WTAP)
products short name :
[Wilms Tumor 1 Associated Protein]
products name syn :
[hFL(2)D; Pre-mRNA-Splicing Regulator WTAP; Female-lethal(2)D homolog; WT1-associated protein]
other names :
[Wilms tumor 1 associated protein; Pre-mRNA-splicing regulator WTAP; pre-mRNA-splicing regulator WTAP; Wilms tumor 1 associated protein; Female-lethal(2)D homolog; hFL(2)D; WT1-associated protein; Wilms tumor 1-associating protein]
products gene name :
[WTAP]
other gene names :
[WTAP; WTAP; Mum2; KIAA0105; hFL(2)D]
uniprot entry name :
FL2D_HUMAN
form :
100mMNaHCO3, 500mMNaCl, pH8.3, containing 0.01% SKL, 5% Trehalose.
concentration :
Original Concentration: 200 ug/mL
storage stability :
Avoid repeated freeze/thaw cycles. Store at 2-8ºC for one month. Aliquot and store at -80ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
tested application :
SDS-PAGE, Western Blot (WB), Positive Control , Immunogen . (May be suitable for use in other assays to be determined by the end user.)
image1 heading :
SDS-Page
image2 heading :
Testing Data (TD)
other info1 :
Organism Species: Homo sapiens (Human). Source: Prokaryotic expression. Residues: Met1~Asp150. Tags: N-terminal His Tag . Subcellular Location: Nucleus. Traits: Freeze-dried powder
other info2 :
Predicted isoelectric point: 5.2. Usage: Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.
ncbi acc num :
AAH69192.1
ncbi mol weight :
Predicted Molecular Mass: 21.3kDa. Accurate Molecular Mass: 21kDa as determined by SDS-PAGE reducing conditions.
ncbi pathways :
Gene Expression Pathway (1269649); Processing Of Capped Intron-Containing Pre-mRNA Pathway (1269688)
ncbi summary :
The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. This gene encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in several transcript variants encoding three different isoforms. [provided by RefSeq, Jul 2012]
uniprot summary :
WTAP: Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA- binding ability and inhibits expression of WT1 target genes. May be involved in mRNA splicing regulation. Belongs to the fl(2)d family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Nucleolus; Spliceosome. Chromosomal Location of Human Ortholog: 6q25-q27. Cellular Component: nuclear membrane; nuclear speck; nucleoplasm; nucleus. Molecular Function: protein binding. Biological Process: cell cycle; gene expression; mRNA processing; regulation of alternative nuclear mRNA splicing, via spliceosome; RNA splicing