protein

Recombinant E1A Binding Protein P300 (EP300)

MBS2032961

0.01 mg

150 USD

Recombinant Protein

Recombinant E1A Binding Protein P300 (EP300)

[E1A Binding Protein P300]

[KAT3B; p300; Histone Acetyltransferase p300]

[E1A binding protein p300; Histone acetyltransferase p300; histone acetyltransferase p300; E1A binding protein p300; E1A-associated protein p300]

[EP300]

[EP300; EP300; p300; KAT3B; RSTS2; P300; p300 HAT]

EP300_HUMAN

E.coli

2414

MAENVVEPGP PSAKRPKLSS PALSASASDG TDFGSLFDLE HDLPDELINS TELGLTNGGD ISQLQTSLGI VQDAASKHKQ LSELLRSGSS PNLNMGVGGP GQVMASQAQQ NSPGLSLINS MVKSPMAQTG LTSPNMGMGS SGPNQGPTQS TAGMMNSP

> 80%

Original Concentration: 200 ug/mL

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8ºC for one month. Aliquot and store at -80ºC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Positive Control; Immunogen; SDS-PAGE; WB. (May be suitable for use in other assays to be determined by the end user.)

SDS-Page

Organism Species: Rattus norvegicus (Rat). Source: Prokaryotic expression. Residues: Met1~Pro158. Tags: N-terminal His and GST Tag. Subcellular Location: Cytoplasm. Traits: Freeze-dried powder. Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.

Predicted isoelectric point: 4.6. Predicted Molecular Mass: 46.0kDa. Accurate Molecular Mass: 46kDa as determined by SDS-PAGE reducing conditions. Usage: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

119580812

EAW60408.1

ATF-2 Transcription Factor Network Pathway (138006); Activation Of HOX Genes During Differentiation Pathway (1339139); Activation Of Anterior HOX Genes In Hindbrain Development During Early Embryogenesis Pathway (1339140); Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Androgen Receptor Signaling Pathway (198806); Attenuation Phase Pathway (1270424); B-WICH Complex Positively Regulates RRNA Expression Pathway (1339126); BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway (1269872); C-MYB Transcription Factor Network Pathway (138073)

This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. [provided by RefSeq, Jul 2008]

p300: a histone acetyltransferase and transcriptional co-activator that regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Related to CPB (CREB-binding protein), and like CPB can stimulate transcription through activation of CREB. Specifically inhibited by the adenovirus oncoprotein E1A. A co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Methylated at R580 and R604 in the KIX domain by CARM1, which blocks association with CREB, inhibits CREB signaling and activates the apoptotic response. Also methylated at R2142 by CARM1, which impairs interaction with NCoA2. Protein type: Transcription, coactivator/corepressor; Nuclear receptor co-regulator; Acetyltransferase; Motility/polarity/chemotaxis; EC 2.3.1.48. Chromosomal Location of Human Ortholog: 22q13.2. Cellular Component: cytoplasm; histone acetyltransferase complex; nucleoplasm; nucleus; transcription factor complex. Molecular Function: acetyltransferase activity; androgen receptor binding; beta-catenin binding; chromatin binding; chromatin DNA binding; damaged DNA binding; DNA binding; histone acetyltransferase activity; lysine N-acetyltransferase activity; nuclear hormone receptor binding; p53 binding; protein binding; protein C-terminus binding; transcription activator binding; transcription coactivator activity; transcription factor binding; transferase activity, transferring acyl groups; zinc ion binding. Biological Process: apoptosis; B cell differentiation; circadian rhythm; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; DNA repair; establishment and/or maintenance of chromatin architecture; fat cell differentiation; G2/M transition of mitotic cell cycle; gene expression; heart development; innate immune response; internal peptidyl-lysine acetylation; internal protein amino acid acetylation; lung development; mitotic cell cycle; N-terminal peptidyl-lysine acetylation; negative regulation of transcription from RNA polymerase II promoter; nervous system development; Notch signaling pathway; nucleotide-excision repair; organ morphogenesis; platelet formation; positive regulation of gene expression, epigenetic; positive regulation of interferon type I production; positive regulation of protein binding; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription of target genes involved in unfolded protein response; protein amino acid acetylation; protein stabilization; regulation of cell cycle; regulation of gene expression, epigenetic; regulation of transcription, DNA-dependent; response to estrogen stimulus; response to hypoxia; skeletal muscle development; somitogenesis; stimulatory C-type lectin receptor signaling pathway; transcription from RNA polymerase II promoter; transcription-coupled nucleotide-excision repair; viral reproduction. Disease: Colorectal Cancer; Rubinstein-taybi Syndrome 1; Rubinstein-taybi Syndrome 2

0.01 mg

150 USD

0.05 mg

290

0.1 mg

450

0.2 mg

560

0.5 mg

1080

1 mg

1605