protein

Peroxiredoxin 2, 1-198aa, Human, Recombinant, E Coli

MBS203290

0.1 mg

375 USD

Recombinant Protein

Peroxiredoxin 2, 1-198aa, Human, Recombinant, E Coli

Peroxiredoxin 2

NKEFB; PRP; PRX2; PRXII; TDPX1; TSA; PRDX2; Peroxiredoxin 2; MGC4104; Natural killer cell enhancing factor B; Natural Killer Enhancing Factor B; NKEF B; PRDX 2; TDPX1; Thiol Specific Antioxidant 1; Thiol specific antioxidant protein; Thioredoxin Dependent Peroxide Reductase 1; Thioredoxin Peroxidase 1; Torin.

peroxiredoxin-2; Peroxiredoxin-2; peroxiredoxin-2; epididymis secretory sperm binding protein Li 2a; natural killer cell-enhancing factor B; thiol-specific antioxidant 1; thioredoxin peroxidase 1; thioredoxin-dependent peroxide reductase 1; torin; peroxiredoxin 2; Natural killer cell-enhancing factor B; NKEF-B; PRP; Thiol-specific antioxidant protein; TSA; Thioredoxin peroxidase 1; Thioredoxin-dependent peroxide reductase 1

PRDX2; PRDX2; PRP; TSA; PRX2; PTX1; TPX1; NKEFB; PRXII; TDPX1; NKEF-B; HEL-S-2a; NKEFB; TDPX1; NKEF-B; TSA

PRDX2_HUMAN

198

MASGNARIGK PAPDFKATAV VDGAFKEVKL SDYKGKYVVL FFYPLDFTFV CPTEIIAFSN RAEDFRKLGC EVLGVSVDSQ FTHLAWINTP RKEGGLGPLN IPLLADVTRR LSEDYGVLKT DEGIAYRGLF IIDGKGVLRQ ITVNDLPVGR SVDEALRLVQ AFQYTDEHGE VCPAGWKPGS DTIKPNVDDS KEYFSKHN

> 90% by SDS - PAGE

Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human

Expression System: E Coli. Bioactivity: Specific activity: approximately 200-230 pmole/min/ug. Enzymatic activity was confirmed by measuring the remaining peroxide after incubation of PRDX2 and peroxide for 20 min at room temperature. Specific activity is defined as the amount of hydroperoxide that 1ug of enzyme can reduce at 25 C for 1 minute. Assay: 1. Prepare a 50ul reaction mix into a suitable container : . The final concentrations are 1mM DTT, 0.03X PBS, 0.5% glycerol. 2. Add 5 ul of recombinant PRDX2 solution with various concentrations (0.25ug, 0.5ug) in 45 ul reaction buffer. 3. Incubate at 25C for 2 minutes. 4. Add 5ul of 5 mM H2O2 as a substrate and incubate the mixture for 20 min. 5. Add 20ul of 26 % trichloroacetic acid (TCA) to stop the reaction. 6. Add 30ul of Formation solution (10mM Ferrous ammonium sulfate (Fe(II)(NH4)2(SO4)2), 2.5M KSCN) . 7. Record the increase in A475nm.

Redox Proteins

Peroxiredoxin 2, also known as PRDX2, is a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. Peroxiredoxin 2 may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. If Peroxiredoxin 2 protection is inadequate against peroxidases, the resulting protein and DNA damage may result in neurological disease such as Alzheimer's or DNA damage leading to cancer. Recombinant human Peroxiredoxin 2 protein was expressed in E.coli and purified by using conventional chromatography.

Kim K., et al. (2009), Oncol Rep, 21(6):1391-6. Kim JH., et al. (2008). Clin Cancer Res. 14(8):2326-33.

32189392

NP_005800

NM_005809.5

21.8 kDa (198aa), confirmed by MALDI-TOF.

Cellular Responses To Stress Pathway (645258); Detoxification Of Reactive Oxygen Species Pathway (1127552); Selenium Pathway (198825)

This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein plays an antioxidant protective role in cells, and it may contribute to the antiviral activity of CD8(+) T-cells. The crystal structure of this protein has been resolved to 2.7 angstroms. This protein prevents hemolytic anemia from oxidative stress by stabilizing hemoglobin, thus making this gene a therapeutic target for patients with hemolytic anemia. This protein may have a proliferative effect and play a role in cancer development or progression. Related pseudogenes have been identified on chromosomes 5, 6, 10 and 13. [provided by RefSeq, Mar 2013]

PRDX2: a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2). May have a proliferative effect and play a role in cancer development or progression. Three splice-variant isoforms have been identified. Protein type: Oxidoreductase; EC 1.11.1.15. Chromosomal Location of Human Ortholog: 19p13.2. Cellular Component: cytoplasm; cytosol. Molecular Function: antioxidant activity; thioredoxin peroxidase activity. Biological Process: regulation of apoptosis; response to reactive oxygen species; removal of superoxide radicals; hydrogen peroxide catabolic process; negative regulation of neuron apoptosis; response to oxidative stress; negative regulation of apoptosis

0.1 mg

375 USD

0.5 mg

925