protein

ALDH2,18-517aa, Human, Recombinant, E Coli

MBS203287

0.1 mg

255 USD

Recombinant Protein

ALDH2,18-517aa, Human, Recombinant, E Coli

ALDH2

ALDH-E2; ALDHI; ALDM; Aldehyde dehydrogenase; mitochondrial Acetaldehyde dehydrogenase 2; Aldehyde dehydrogenase 2 family; Aldehyde dehydrogenase 2 family (mitochondrial); Aldehyde dehydrogenase mitochondrial; ALDH 2; ALDH class 2; ALDH E2; Liver mitochondrial ALDH; MGC1806; Mitochondrial aldehyde dehydrogenase 2; Nucleus encoded mitochondrial aldehyde dehydrogenase 2.

aldehyde dehydrogenase, mitochondrial isoform 1; Aldehyde dehydrogenase, mitochondrial; aldehyde dehydrogenase, mitochondrial; ALDH class 2; acetaldehyde dehydrogenase 2; liver mitochondrial ALDH; nucleus-encoded mitochondrial aldehyde dehydrogenase 2; aldehyde dehydrogenase 2 family (mitochondrial); ALDH class 2; ALDH-E2; ALDHI

ALDH2

ALDH2; ALDH2; ALDM; ALDHI; ALDH-E2; ALDM

ALDH2_HUMAN

517

MSAAATQAVP APNQQPEVFC NQIFINNEWH DAVSRKTFPT VNPSTGEVIC QVAEGDKEDV DKAVKAARAA FQLGSPWRRM DASHRGRLLN RLADLIERDR TYLAALETLD NGKPYVISYL VDLDMVLKCL RYYAGWADKY HGKTIPIDGD FFSYTRHEPV GVCGQIIPWN FPLLMQAWKL GPALATGNVV VMKVAEQTPL TALYVANLIK EAGFPPGVVN IVPGFGPTAG AAIASHEDVD KVAFTGSTEI GRVIQVAAGS SNLKRVTLEL GGKSPNIIMS DADMDWAVEQ AHFALFFNQG QCCCAGSRTF VQEDIYDEFV ERSVARAKSR VVGNPFDSKT EQGPQVDETQ FKKILGYINT GKQEGAKLLC GGGIAADRGY FIQPTVFGDV QDGMTIAKEE IFGPVMQILK FKTIEEVVGR ANNSTYGLAA AVFTKDLDKA NYLSQALQAG TVWVNCYDVF GAQSPFGGYK MSGSGRELGE YGLQAYTEVK TVTVKVPQKN S

> 90% by SDS-PAGE

Liquid. In 20 mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 1 mM EDTA, 10% glycerol

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human

Expression System: E Coli. Bioactivity: Specific activity is > 0.14 units/ml, and was obtained by measuring the increase of NADP in absorbance at 340 nm resulting from the reduction of NAD. One unit will oxidize 1.0 umole of acetaldehyde to acetic acid per minute at pH 8.0 at 25C in the presence of beta-NAD, potassium and thiols. Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method). Assay: 1. Prepare a 3ml reaction mixture into a suitable container: The final concentrations are 103 mM Tris, 0.67 mM beta-NAD, 100 mM potassium chloride, 10 mM 2-mercaptoethanol, 2 mM acetaldehyde, 0.0007 % (w/v) BSA. 2. Equilibrate to 25C and monitor at A340nm until value is constant using a spectrophotometer. 3. Add 5ug of recombinant ALDH2 into reaction mixture and mix immediately. 4. Record the increase at A340nm for 5 minutes.

Redox Proteins

ALDH2 (Aldehyde dehydrogenase 2 family) belongs to the aldehyde dehydrogenase family which catalyze the chemical transformation from acetaldehyde to acetic acid and is the second enzyme of the major oxidative pathway of alcohol metabolism. There are two major liver isoforms of this enzyme, cytosolic and mitochondrial, and they can be also distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Recombinant human ALDH2 protein was expressed in E.coli and purified by using conventional chromatography techniques.

Crabb DW., et al. (1989). J Clin Invest. 83(1):314-6. Feng Liu., et al. (2002). Plant Physiol. 130(4):1657-1674.

25777732

NP_000681

NM_000690.3

54.5 kDa (501aa), confirmed by MALDI-TOF.

Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); Ascorbate And Aldarate Metabolism Pathway (82932); Ascorbate And Aldarate Metabolism Pathway (293); Biological Oxidations Pathway (105698); Defective CYP11A1 Causes Adrenal Insufficiency, Congenital, With 46,XY Sex Reversal (AICSR) Pathway (1127640); Defective CYP11B1 Causes Adrenal Hyperplasia 4 (AH4) Pathway (1127641); Defective CYP11B2 Causes Corticosterone Methyloxidase 1 Deficiency (CMO-1 Deficiency) Pathway (1127642); Defective CYP17A1 Causes Adrenal Hyperplasia 5 (AH5) Pathway (1127643); Defective CYP19A1 Causes Aromatase Excess Syndrome (AEXS) Pathway (1127644)

This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Mar 2011]

ALDH2: This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Mar 2011]. Protein type: Amino Acid Metabolism - tryptophan; Carbohydrate Metabolism - propanoate; Amino Acid Metabolism - histidine; Carbohydrate Metabolism - ascorbate and aldarate; Other Amino Acids Metabolism - beta-alanine; Carbohydrate Metabolism - pyruvate; Oxidoreductase; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Mitochondrial; Lipid Metabolism - glycerolipid; Amino Acid Metabolism - lysine degradation; Lipid Metabolism - fatty acid; Secondary Metabolites Metabolism - limonene and pinene degradation; EC 1.2.1.3; Amino Acid Metabolism - arginine and proline; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - butanoate. Chromosomal Location of Human Ortholog: 12q24.2. Cellular Component: mitochondrial matrix. Molecular Function: aldehyde dehydrogenase (NAD) activity; electron carrier activity; aldehyde dehydrogenase [NAD(P)+] activity. Biological Process: synaptic transmission; ethanol catabolic process; xenobiotic metabolic process; alcohol metabolic process; carbohydrate metabolic process; ethanol oxidation; neurotransmitter biosynthetic process. Disease: Alcohol Sensitivity, Acute

0.1 mg

255 USD

0.5 mg

535