Aldose reductase, 1-316aa, Human, Recombinant, E Coli | MyBioSource MBS203284 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Aldose reductase, 1-316aa, Human, Recombinant, E Coli

catalog :

MBS203284

quantity :

0.1 mg

price :

255 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203284

products type :

Recombinant Protein

products full name :

Aldose reductase, 1-316aa, Human, Recombinant, E Coli

products short name :

Aldose reductase

products name syn :

AKR1B1; ADR; ALDR1; ALR2; AR; Aldo-keto reductase family1 member B1; Aldose reductase; Aldo-keto reductase family1; member B1 AKR1B 1; Aldehyde reductase; aldehyde reductase 1; aldo-keto reductase family 1; member B1 aldr 1; aldr-1; Lii5-2 CTCL tumor antigen; low Km aldose reductase.

other names :

aldose reductase; Aldose reductase; aldose reductase; Lii5-2 CTCL tumor antigen; aldehyde reductase 1; aldo-keto reductase family 1 member B1; low Km aldose reductase; aldo-keto reductase family 1, member B1 (aldose reductase); Aldehyde reductase; Aldo-keto reductase family 1 member B1

other gene names :

AKR1B1; AKR1B1; AR; ADR; ALR2; ALDR1; ALDR1; AR

uniprot entry name :

ALDR_HUMAN

sequence length :

316

sequence :

MASRLLLNNG AKMPILGLGT WKSPPGQVTE AVKVAIDVGY RHIDCAHVYQ NENEVGVAIQ EKLREQVVKR EELFIVSKLW CTYHEKGLVK GACQKTLSDL KLDYLDLYLI HWPTGFKPGK EFFPLDESGN VVPSDTNILD TWAAMEELVD EGLVKAIGIS NFNHLQVEMI LNKPGLKYKP AVNQIECHPY LTQEKLIQYC QSKGIVVTAY SPLGSPDRPW AKPEDPSLLE DPRIKAIAAK HNKTTAQVLI RFPMQRNLVV IPKSVTPERI AENFKVFDFE LSSQDMTTLL SYNRNWRVCA LLSCTSHKDY PFHEEF

purity :

> 95% by SDS - PAGE

form :

Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human

other info2 :

Expression System: E Coli. Bioactivity: Specific activity: approximately 0.2-0.9 units/mg. Enzymatic activity was confirmed by measuring the amount of enzyme catalyzing the oxidation of 1 micromole NADPH/min at 25C. Specific activity was expressed as units/mg protein. Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method). Assay: 1. Prepare a 1ml reaction mix into a suitable container : The final concentrations are 0.1M sodium phosphate (pH7.0), 10mM DL-glyceraldehyde, 0.3mM NADPH. 2. Add 50ul of recombinant AKR1B1 solution with various concentrations (2.5ug, 5ug, 10ug) in 750ul reaction buffer. 3. Mix by inversion and Incubate at 25C for 2.5 minutes. 4. Add 200ul of 50 mM DL-glyceraldehyde as a substrate and immediately mix by inversion. 5. Record the increase at A340nm for 3 minutes.

products categories :

Redox Proteins

products description :

Aldose reductase (AKR1B1) is a member of the aldo-keto reductase (AKR) superfamily and catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. This protein is implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Recombinant Aldose reductase (AKR1B) protein was expressed in E.coli and purified by using conventional chromatography techniques.

products references :

Ruiz FX., et al: (2009) Chem Biol Interact.178(1-3). Donaqhue KC., et al: Diabet Med. 22(10):1315-20.

ncbi gi num :

4502049

ncbi acc num :

NP_001619

ncbi gb acc num :

NM_001628.2

ncbi mol weight :

35.8kDa (316aa), confirmed by MALDI-TOF.

ncbi pathways :

Fructose And Mannose Metabolism Pathway (82930); Fructose And Mannose Metabolism Pathway (291); Galactose Metabolism Pathway (82931); Galactose Metabolism Pathway (292); Glycerolipid Metabolism Pathway (82986); Glycerolipid Metabolism Pathway (361); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Metabolism Of Steroid Hormones And Vitamin D Pathway (106150)

ncbi summary :

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Multiple pseudogenes have been identified for this gene. The nomenclature system used by the HUGO Gene Nomenclature Committee to define human aldo-keto reductase family members is known to differ from that used by the Mouse Genome Informatics database. [provided by RefSeq, Feb 2009]

uniprot summary :

AKR1B1: Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies. Belongs to the aldo/keto reductase family. Protein type: Carbohydrate Metabolism - galactose; Oxidoreductase; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - pentose and glucuronate interconversions; EC 1.1.1.21; Lipid Metabolism - glycerolipid; Carbohydrate Metabolism - fructose and mannose. Chromosomal Location of Human Ortholog: 7q35. Cellular Component: nucleoplasm; extracellular space; cytoplasm; cytosol. Molecular Function: glyceraldehyde oxidoreductase activity; electron carrier activity; aldo-keto reductase activity; aldehyde reductase activity. Biological Process: steroid metabolic process; carbohydrate metabolic process; response to stress; C21-steroid hormone biosynthetic process

size1 :

0.1 mg

price1 :

255 USD

size2 :

0.5 mg

price2 :

535

more info or order :

MyBioSource product webpage