protein

SOD1, 1-154aa, Human, Recombinant, E Coli

MBS203282

0.1 mg

310 USD

Recombinant Protein

SOD1, 1-154aa, Human, Recombinant, E Coli

SOD1

ALS; SOD; ALS1; IPOA; Cu-Zn superoxide dismutase; Superoxide dismutase 1 soluble; SOD1; Superoxide dismutase 1; soluble ALS 1; Amyotrophic lateral sclerosis 1 Amyotrophic lateral sclerosis 1 adult; Cu/Zn SOD; Cu/Zn superoxide dismutase; Homodimer; Indophenoloxidase A; Mn superoxide dismutase; SOD 1; SOD soluble; SOD2; SODC; Superoxide dismutase 1 soluble; Superoxide dismutase Cu Zn; Superoxide dismutase cystolic

UDP-glucuronosyltransferase 1-1; UDP-glucuronosyltransferase 1-1; UDP-glucuronosyltransferase 1-1; UDP glycosyltransferase 1 family, polypeptide A1; UDP-glucuronosyltransferase 1-A; UDP-glucuronosyltransferase 1A1; UGT-1A; UGT1*1; UGT1-01; bilirubin UDP-glucuronosyltransferase 1-1; bilirubin UDP-glucuronosyltransferase isozyme 1; bilirubin-specific UDPGT isozyme 1; UDP glucuronosyltransferase 1 family, polypeptide A1; Bilirubin-specific UDPGT isozyme 1; hUG-BR1; UDP-glucuronosyltransferase 1-A; UGT-1A; UGT1A; UDP-glucuronosyltransferase 1A1

SOD1

UGT1A1; UGT1A1; GNT1; UGT1; UDPGT; UGT1A; HUG-BR1; BILIQTL1; UDPGT 1-1; GNT1; UGT1; UDPGT 1-1; UGT1*1; UGT1-01; UGT1.1; hUG-BR1; UGT-1A; UGT1A

UD11_HUMAN

533

MATKAVCVLK GDGPVQGIIN FEQKESNGPV KVWGSIKGLT EGLHGFHVHE FGDNTAGCTS AGPHFNPLSR KHGGPKDEER HVGDLGNVTA DKDGVADVSI EDSVISLSGD HCIIGRTLVV HEKADDLGKG GNEESTKTGN AGSRLACGVI GIAQ

> 95% by SDS - PAGE

Liquid in 20 mM Tris pH 7.5, 10% Glycerol.

1 mg/ml (determined by Bradford assay)

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

SDS-PAGE

Antigen Species: Human

Expression System: E Coli. Bioactivity: Specific activity is > 90 units/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and Xanthine oxidase at pH 7.8 at 25C in a 1.5 ml reaction volume. Assay: 1. Prepare a 1.5 ml reaction mix into a suitable container and pre-chill on ice before use: The final concentrations are 50mM potassium phosphate, 0.1mM ethylendiaminetetraacetic acid, 0.01mM cytochromC 0.05mM xanthine, 0.005 units xanthine oxidase. 2. Equilibrate to 25C and monitor at A550nm until the value is constant using a spectrophotometer. 3. Add 50 ul of recombinant SOD protein in various concentrations (0.5ug, 1ug) in assay buffer. 4. Mix by inversion and record the increase at A550nm for 5 minutes.

Redox Proteins

Superoxide dismutase 1(SOD1) binds copper and zinc ions and is one of three isozymes responsible for destroying free superoxide radicals in the body. The encoded protein neutralizes supercharged oxygen molecules, which can damage cells if their levels are not controlled. Mutations in SOD1 cause a form of familial amyotrophic lateral sclerosis (ALS). Recombinant SOD1 was expressed in E.coli and purified by conventional chromatography techniques.

Conwit R., et al. (2006) Journal of the Neurological Sciences 251 (1-2). Banci L., et al. (2008) PLoS ONE. 3(2):e1677. This product was used in the following article:. Kwon MJ et.al Superoxide dismutase 3 suppresses hyaluronic http://www.ncbi.nlm.nih.gov/pubmed/21957979.

8850236

NP_000454

NM_000463.2

15.9 kDa (154 aa), confirmed by MALDI-TOF.

AhR Pathway (755436); Ascorbate And Aldarate Metabolism Pathway (82932); Ascorbate And Aldarate Metabolism Pathway (293); Biological Oxidations Pathway (105698); Chemical Carcinogenesis Pathway (673221); Chemical Carcinogenesis Pathway (673237); Codeine And Morphine Metabolism Pathway (198831); Defective AHCY Causes Hypermethioninemia With S-adenosylhomocysteine Hydrolase Deficiency (HMAHCHD) Pathway (1127639); Defective GCLC Causes Hemolytic Anemia Due To Gamma-glutamylcysteine Synthetase Deficiency (HAGGSD) Pathway (1127658); Defective GGT1 Causes Glutathionuria (GLUTH) Pathway (1127659)

This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. The preferred substrate of this enzyme is bilirubin, although it also has moderate activity with simple phenols, flavones, and C18 steroids. Mutations in this gene result in Crigler-Najjar syndromes types I and II and in Gilbert syndrome. [provided by RefSeq, Jul 2008]

UGT1A1: UDPGT is of major importance in the conjugation and subsequent elimination of potentially toxic xenobiotics and endogenous compounds. This isoform glucuronidates bilirubin IX- alpha to form both the IX-alpha-C8 and IX-alpha-C12 monoconjugates and diconjugate. Is also able to catalyze the glucuronidation of 17beta-estradiol, 17alpha-ethinylestradiol, 1-hydroxypyrene, 4- methylumbelliferone, 1-naphthol, paranitrophenol, scopoletin, and umbelliferone. Part a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Expressed in liver. Not expressed in skin or kidney. Belongs to the UDP-glycosyltransferase family. 1 isoforms of the human protein are produced by alternative splicing. Protein type: Xenobiotic Metabolism - metabolism by cytochrome P450; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Carbohydrate Metabolism - starch and sucrose; Cofactor and Vitamin Metabolism - retinol; Carbohydrate Metabolism - pentose and glucuronate interconversions; Xenobiotic Metabolism - drug metabolism - other enzymes; Lipid Metabolism - androgen and estrogen; Carbohydrate Metabolism - ascorbate and aldarate; EC 2.4.1.17; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; Membrane protein, integral; Transferase. Chromosomal Location of Human Ortholog: 2q37. Cellular Component: endoplasmic reticulum membrane; integral to plasma membrane; endoplasmic reticulum. Molecular Function: enzyme inhibitor activity; retinoic acid binding; enzyme binding; protein homodimerization activity; protein heterodimerization activity; glucuronosyltransferase activity; steroid binding. Biological Process: steroid metabolic process; response to drug; estrogen metabolic process; negative regulation of steroid metabolic process; organ regeneration; response to lipopolysaccharide; negative regulation of fatty acid metabolic process; liver development; cellular response to hormone stimulus; response to starvation; negative regulation of transferase activity; bilirubin conjugation; flavonoid biosynthetic process; heme catabolic process; xenobiotic metabolic process; negative regulation of catalytic activity; digestion; porphyrin metabolic process; acute-phase response; flavone metabolic process; retinoic acid metabolic process; heterocycle metabolic process; drug metabolic process; response to nutrient. Disease: Gilbert Syndrome; Crigler-najjar Syndrome, Type I; Bilirubin, Serum Level Of, Quantitative Trait Locus 1; Crigler-najjar Syndrome, Type Ii; Hyperbilirubinemia, Transient Familial Neonatal

0.1 mg

310 USD

0.5 mg

720