catalog number :
MBS203280
products type :
Recombinant Protein
products full name :
Thioredoxin2, 60-166aa, Human, Recombinant, E Coli
products short name :
Thioredoxin2
products name syn :
MTRX; TRX2; MT-TRX; Thioredoxin2; mitochondrial thioredoxin; thioredoxin 2 precursor; Thioredoxin mitochondrial; TRX 2; TXN 2; TXN2; Thioredoxin-2
other names :
thioredoxin, mitochondrial; Thioredoxin, mitochondrial; thioredoxin, mitochondrial; mitochondrial thioredoxin; thioredoxin-2; thioredoxin 2; Thioredoxin-2
other gene names :
TXN2; TXN2; MTRX; TRX2; MT-TRX; TRX2; MTRX; Mt-Trx
uniprot entry name :
THIOM_HUMAN
sequence :
MTTFNIQDGP DFQDRVVNSE TPVVVDFHAQ WCGPCKILGP RLEKMVAKQH GKVVMAKVDI DDHTDLAIEY EVSAVPTVLA MKNGDVVDKF VGIKDEDQLE AFLKKLIG
purity :
> 95% by SDS - PAGE
form :
Liquid in Phosphate-Buffered Saline (pH 7.4)
concentration :
1 mg/ml (determined by Bradford assay)
storage stability :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
tested application :
SDS-PAGE
other info1 :
Antigen Species: Human
other info2 :
Expression System: E Coli. Bioactivity: Specific activity is 3-4 A650/min/mg, obtained by measuring the increase of insulin precipitation in absorbance at 650 nm resulting from the reduction of insulin. Assay: 1. Prepare a 1ml reaction cocktail into a suitable container: The final concentrations are 5mM Tris, 63mM sodium phosphate, 1mM DTT, 0.1% (w/v) insulin, 2mM EDTA. 2. Add 4ug of recombinant thioredoxin2 into reaction cocktail and mix immediately. 3. Incubate at 25C for 20 minutes. 4. Record the increase in A650nm for 15 minutes.
products categories :
Redox Proteins
products description :
Thioredoxin(Trx) is a low molecular weight redox protein. Trx contains a redox active disulfide/dithiol group within the conserved Cys-Gly-Pro-Cys active site. The Thioredoxin2 may play important roles in the regulation of the mitochondrial membrane potential and in protection against oxidant-induced apoptosis. Recombinant Thioredoxin2 was expressed in E.coli and purified by using conventional chromatography techniques.
products references :
Wang Z., et al. (2007). Rapid Commun Mass Spectrom. 21(22):3658-66. Zhang H., et al. (2007). Arch Biochem biophys. 465(1):119-26.
ncbi gb acc num :
NM_012473.3
ncbi mol weight :
11 kDa (108 aa), confirmed by MALDI-TOF.
ncbi pathways :
Cellular Responses To Stress Pathway 645258!!Detoxification Of Reactive Oxygen Species Pathway 1127552!!Oxidative Stress Pathway 198916
ncbi summary :
This nuclear gene encodes a mitochondrial member of the thioredoxin family, a group of small multifunctional redox-active proteins. The encoded protein may play important roles in the regulation of the mitochondrial membrane potential and in protection against oxidant-induced apoptosis. [provided by RefSeq, Jul 2008]