product summary
Loading...
company name :
MyBioSource
product type :
protein
product name :
GFP, 1-238aa, Aequorea victoria, Recombinant, E Coli
catalog :
MBS203279
quantity :
0.1 mg
price :
290 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS203279
products type :
Recombinant Protein
products full name :
GFP, 1-238aa, Aequorea victoria, Recombinant, E Coli
products short name :
GFP
products name syn :
Green fluorescent protein
other names :
Green fluorescent protein; Green fluorescent protein
products gene name :
GFP
other gene names :
GFP
uniprot entry name :
GFP_AEQVI
sequence length :
238
sequence :
MSKGEELFTG VVPILVELDG DVNGHKFSVS GEGEGDATYG KLTLKFICTT GKLPVPWPTL VTTFSYGVQC FSRYPDHMKQ HDFFKSAMPE GYVQERTIFF KDDGNYKTRA EVKFEGDTLV NRIELKGIDF KEDGNILGHK LEYNYNSHNV YIMADKQKNG IKVNFKIRHN IEDGSVQLAD HYQQNTPIGD GPVLLPDNHY LSTQSALSKD PNEKRDHMVL LEFVTAAGIT HGMDELYK
purity :
> 95% by SDS - PAGE
form :
Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
concentration :
1 mg/ml (determined by Bradford assay)
storage stability :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
tested application :
SDS-PAGE
other info1 :
Antigen Species: Aequorea victoria
other info2 :
Expression System: E Coli. Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method). Analysis: 1. Prepare a 150ul recombinant GFP with various concentrations (0.00074 nM - 6.21uM) in assay buffer and equilibrate to 25C. (Assay buffer: 10 mM Tris-HCl (pH 8.0), 10 mM EDTA, 0.02% sodium azide.) . 2. Read at excitation wavelengths 485nm and emission 535nm. - 96 Well Polystyrene Microplate, black (greiner bio-one) . - Fluorescent plate reader (PerkinEmerl, VICTOR X3)
products categories :
Fusion Tag & Detection
products description :
GFP, also known as green fluorescent protein, is a protein produced by the jellyfish (Aequorea Victoria) that emits bioluminescence in the green zone of the visible spectrum. GFP has become a useful and ubiquitous tool for making chimeric proteins, where it functions as a fluorescent protein tag. It has been expressed in most known cell types and is used as a noninvasive fluorescent marker in living cells and organisms. This protein enables a wide range of applications where it has functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Recombinant GFP protein was expressed in E.coli and purified by using conventional chromatography.
products references :
Millan MI, et al. (2009). Medicina. 69(3):370-4. Marshall J, et al. (1995). Neuron, 14(2):211-5.
ncbi gi num :
1169893
ncbi acc num :
P42212.1
uniprot acc num :
P42212
ncbi mol weight :
26.8 kDa (238aa), confirmed by MALDI-TOF.
size1 :
0.1 mg
price1 :
290 USD
size2 :
0.5 mg
price2 :
650
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

Would you like to receive email and e-newsletter from MyBioSource about new products, special offers and events? Please click here to join our Mailing List!