DHH, 23-198aa, Human, His-tag, Recombinant, E Coli | MyBioSource MBS203272 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

DHH, 23-198aa, Human, His-tag, Recombinant, E Coli

catalog :

MBS203272

quantity :

0.1 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203272

products type :

Recombinant Protein

products full name :

DHH, 23-198aa, Human, His-tag, Recombinant, E Coli

products short name :

DHH

products name syn :

Drosophila; HHG-3; MGC35145; Desert hedgehog protein N-product; DHH; desert hedgehog preproprotein Desert hedgehog; Desert hedgehog (Drosophila) homolog; Desert hedgehog homolog (Drosophila); Desert hedgehog protein precursor.

other names :

desert hedgehog protein preproprotein; Desert hedgehog protein; desert hedgehog protein; desert hedgehog homolog; mutant desert hedgehog; desert hedgehog; HHG-3Cleaved into the following 2 chains:Desert hedgehog protein N-product; Desert hedgehog protein C-product

products gene name :

DHH

other gene names :

DHH; DHH; GDXYM; HHG-3; SRXY7; DHH

uniprot entry name :

DHH_HUMAN

sequence length :

396

sequence :

MGSSHHHHHH SSGLVPRGSH MCGPGRGPVG RRRYARKQLV PLLYKQFVPG VPERTLGASG PAEGRVARGS ERFRDLVPNY NPDIIFKDEE NSGADRLMTE RCKERVNALA IAVMNMWPGV RLRVTEGWDE DGHHAQDSLH YEGRALDITT SDRDRNKYGL LARLAVEAGF DWVYYESRNH VHVSVKADNS LAVRAGG

purity :

> 95% by SDS - PAGE

form :

Liquid in 20mM MES pH 5.5, 0.5mM DTT, 20% glycerol

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli. Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method)

products categories :

Signal Transduction

products description :

DHH belongs to the hedgehog family. The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. The N- terminal domain is the active species in both local and long-range signaling, whereas the C-terminal domain has no signaling activity. This protein is produced by Sertoli cells and may be involved in both male gonadal differentiation and perineurial development. Defects in this protein have been associated with partial gonadal dysgenesis (PGD) accompanied by minifascicular polyneuropathy. Recombinant DHH protein was expressed in E.coli and purified by using conventional chromatography techniques.

products references :

Chen YJ, et al. (2007). Cell Cycle. 6(15):1826-30. Van den Brink GR., et al. (2007). Physiol Rev. 87(4):1343-75.

ncbi gi num :

19482158

ncbi acc num :

NP_066382

ncbi gb acc num :

NM_021044.2

ncbi mol weight :

22kDa (197aa), confirmed by MALDI-TOF.

ncbi pathways :

Activation Of SMO Pathway (1127544); Class B/2 (Secretin Family Receptors) Pathway (106378); Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway (1127664); Disease Pathway (530764); GPCR Ligand Binding Pathway (161020); Hedgehog 'on' State Pathway (1127542); Hedgehog Signaling Pathway (198835); Hedgehog Ligand Biogenesis Pathway (1127536); Hedgehog Signaling Pathway (1084754); Hedgehog Signaling Pathway (1108217)

ncbi summary :

This gene encodes a member of the hedgehog family. The hedgehog gene family encodes signaling molecules that play an important role in regulating morphogenesis. This protein is predicted to be made as a precursor that is autocatalytically cleaved; the N-terminal portion is soluble and contains the signalling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the organism. Defects in this protein have been associated with partial gonadal dysgenesis (PGD) accompanied by minifascicular polyneuropathy. This protein may be involved in both male gonadal differentiation and perineurial development. [provided by RefSeq, May 2010]

uniprot summary :

DHH: Intercellular signal essential for a variety of patterning events during development. May function as a spermatocyte survival factor in the testes. Essential for testes development. Defects in DHH may be the cause of partial gonadal dysgenesis with minifascicular neuropathy 46,XY (PGD). PGD is characterized by the presence of a testis on one side and a streak or an absent gonad at the other, persistence of Muellerian duct structures, and a variable degree of genital ambiguity. Defects in DHH may be the cause of complete pure gonadal dysgenesis 46,XY type (GDXYM); also known as male- limited gonadal dysgenesis 46,XY. GDXYM is a type of hypogonadism in which no functional gonads are present to induce puberty in an externally female person whose karyotype is then found to be XY. The gonads are found to be non-functional streaks. Belongs to the hedgehog family. Protein type: Secreted; Secreted, signal peptide; Cell development/differentiation. Chromosomal Location of Human Ortholog: 12q13.1. Cellular Component: extracellular space; plasma membrane. Molecular Function: peptidase activity; protein binding; zinc ion binding; patched binding; calcium ion binding. Biological Process: myelination; Leydig cell differentiation; cell-cell signaling; male sex determination; regulation of steroid biosynthetic process; proteolysis; response to estradiol stimulus; spermatid development. Disease: 46,xy Gonadal Dysgenesis, Partial, With Minifascicular Neuropathy; 46,xy Sex Reversal 7

size1 :

0.1 mg

price1 :

310 USD

size2 :

0.5 mg

price2 :

720

more info or order :

MyBioSource product webpage