14-3-3 zeta, 1-245aa, Human, His-tag, Recombinant, E Coli | MyBioSource MBS203271 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

14-3-3 zeta, 1-245aa, Human, His-tag, Recombinant, E Coli

catalog :

MBS203271

quantity :

0.1 mg

price :

310 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203271

products type :

Recombinant Protein

products full name :

14-3-3 zeta, 1-245aa, Human, His-tag, Recombinant, E Coli

products short name :

14-3-3 zeta

products name syn :

YWHAZ; KCIP-1; MGC111427; Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein; zeta polypeptide; 14-3-3 zeta; Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein; zeta polypeptide 14 3 3 protein zeta/delta; KCIP1; Phospholipase A2; Protein kinase C inhibitor protein 1; Tyr-3/Trp-5 monooxygenase Activation protein; Zeta; Tyr3/Trp5 monooxygenase Activation protein; Zeta

other names :

14-3-3 protein zeta/delta; 14-3-3 protein zeta/delta; 14-3-3 protein zeta/delta; 14-3-3 delta; 14-3-3 protein/cytosolic phospholipase A2; 14-3-3 zeta; epididymis luminal protein 4; epididymis secretory protein Li 3; protein kinase C inhibitor protein 1; protein kinase C inhibitor protein-1; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, delta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide; tyrosine 3/tryptophan 5 -monooxygenase activation protein, zeta polypeptide; tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta; Protein kinase C inhibitor protein 1; KCIP-1

products gene name :

14-3-3 zeta

other gene names :

YWHAZ; YWHAZ; HEL4; YWHAD; KCIP-1; HEL-S-3; 14-3-3-zeta; KCIP-1

uniprot entry name :

1433Z_HUMAN

sequence length :

245

sequence :

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMDK NELVQKAKLA EQAERYDDMA ACMKSVTEQG AELSNEERNL LSVAYKNVVG ARRSSWRVVS SIEQKTEGAE KKQQMAREYR EKIETELRDI CNDVLSLLEK FLIPNASQAE SKVFYLKMKG DYYRYLAEVA AGDDKKGIVD QSQQAYQEAF EISKKEMQPT HPIRLGLALN FSVFYYEILN SPEKACSLAK TAFDEAIAEL DTLSEESYKD STLIMQLLRD NLTLWTSDTQ GDEAEAGEGG EN

purity :

> 95% by SDS - PAGE

form :

Liquid in Phosphate Buffered Saline pH7.4

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli

products categories :

Signal Transduction

products description :

The 14-3-3 family of proteins plays a key regulatory role in signal transduction, checkpoint control, apoptotic and nutrient-sensing pathways. 14-3-3 proteins are highly conserved and ubiquitously expressed. There are at least seven isoforms, beta, gamma, epsilon,sigma, zeta, tau and eta that have been identified in mammals. 14-3-3 zeta interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Recombinant human YWHAZ, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

products references :

Gannon-Murakami L., et al. (2002) J Biol Chem. 277(26): 23116-23122. Li FQ., et al. (2008) J Cell Biol. 181(7):1141-54.

ncbi gi num :

21735625

ncbi acc num :

NP_663723

ncbi gb acc num :

NM_145690.2

ncbi mol weight :

32 kDa (245 aa), confirmed by MALDI-TOF.

ncbi pathways :

Activation Of BAD And Translocation To Mitochondria Pathway (105659); Activation Of BH3-only Proteins Pathway (105658); Adaptive Immune System Pathway (366160); Alpha6-Beta4 Integrin Signaling Pathway (198807); Apoptosis Pathway (105648); Calcium Regulation In The Cardiac Cell Pathway (198906); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054); Cell Cycle Pathway (463); Class I PI3K Signaling Events Mediated By Akt Pathway (138020)

ncbi summary :

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq, Oct 2008]

uniprot summary :

14-3-3 zeta: a protein of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. A multifunctional regulator of the cell signaling processes. Phosphorylation apparently disrupts homodimerization. Protein type: Adaptor/scaffold; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 8q23.1. Cellular Component: extracellular space; mast cell granule; cytoplasmic vesicle membrane; focal adhesion; protein complex; mitochondrion; leading edge; postsynaptic density; cytosol; nucleoplasm; perinuclear region of cytoplasm; cytoplasm; melanosome; nucleus. Molecular Function: protein domain specific binding; identical protein binding; protein binding; protein complex binding; transcription factor binding; protein kinase binding. Biological Process: response to drug; platelet activation; histamine secretion by mast cell; establishment of Golgi localization; apoptosis; gene expression; blood coagulation; signal transduction; protein targeting to mitochondrion; negative regulation of apoptosis

size1 :

0.1 mg

price1 :

310 USD

size2 :

0.5 mg

price2 :

720

more info or order :

MyBioSource product webpage