Acid phosphatase 1,1-158aa, Human, His-tag, Recombinant, E Coli | MyBioSource MBS203269 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

Acid phosphatase 1,1-158aa, Human, His-tag, Recombinant, E Coli

catalog :

MBS203269

quantity :

0.1 mg

price :

375 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203269

products type :

Recombinant Protein

products full name :

Acid phosphatase 1,1-158aa, Human, His-tag, Recombinant, E Coli

products short name :

Acid phosphatase 1

products name syn :

Acid phosphatase 1; soluble isoform b; ACP1; HAAP; LMW-PTP; Red cell acid phosphatase 1; Adipocyte acid phosphatase; Acid phosphatase 1; soluble isoform b Acid phosphatase 1 soluble; Adipocyte acid phosphatase Cytoplasmic phosphotyrosyl protein phosphatase; Low molecular weight phosphotyrosine protein phosphatase; Acid phosphatase of erythrocyte; PAP2; Protein tyrosine phosphatase; PTPase; Purple acid phosphatase; Red cell acid phosphatase 1.

other names :

low molecular weight phosphotyrosine protein phosphatase isoform b; Low molecular weight phosphotyrosine protein phosphatase; low molecular weight phosphotyrosine protein phosphatase; LMW-PTP; LMW-PTPase; acid phosphatase of erythrocyte; adipocyte acid phosphatase; cytoplasmic phosphotyrosyl protein phosphatase; low molecular weight cytosolic acid phosphatase; protein tyrosine phosphatase; red cell acid phosphatase 1; acid phosphatase 1, soluble; Adipocyte acid phosphatase; Low molecular weight cytosolic acid phosphatase (EC:3.1.3.2); Red cell acid phosphatase 1

other gene names :

ACP1; ACP1; HAAP; LMW-PTP; LMW-PTPase

uniprot entry name :

PPAC_HUMAN

sequence length :

158

sequence :

MGSSHHHHHH SSGLVPRGSH MAEQATKSVL FVCLGNICRS PIAEAVFRKL VTDQNISENW VIDSGAVSDW NVGRSPDPRA VSCLRNHGIH TAHKARQITK EDFATFDYIL CMDESNLRDL NRKSNQVKTC KAKIELLGSY DPQKQLIIED PYYGNDSDFE TVYQQCVRCC RAFLEKAH

purity :

> 95% by SDS - PAGE

form :

Liquid. In 20 mM MES 6.0, 0.1 mM PMSF, 2 mM EDTA, 10% glycerol

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human. Tag: His-tag

other info2 :

Expression System: E Coli. Bioactivity: Activity tested: > 60,000 unit/mg of protein. One unit is defined as the amount of enzyme that will hydrolyze 1nmole of p-nitrophenyl phosphate per minute at 37C in MES pH5.0 using 10mM of substrate. Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method)

products categories :

Enzymes & Proteases

products description :

Acid phosphatase 1, soluble, also known as ACP1, belongs to the phosphotyrosine protein. It functions as an acid phosphatase and a protein tyrosine phosphatase (PTPase) present in all human tissues, including adipocytes. This enzyme hydrolyzes protein tyrosine phosphate to protein tyrosine and orthophosphate, and also orthophosphoric monoesters to alcohol and orthophosphate. Recombinant ACP1, fused to His-tag at N-terminus, was expressed in E.coli and purified by conventional chromatography techniques.

products references :

De Lorenzo A., et al. (2009) Metabolism.58(3):351-4. Banci M., et al. (2009) Cardiology.113(4):236-242.

ncbi gi num :

6005988

ncbi acc num :

NP_009030

ncbi gb acc num :

NM_007099.3

ncbi mol weight :

20.1 kDa (178 aa), confirmed by MALDI-TOF.

ncbi pathways :

Adherens Junction Pathway (83070); Adherens Junction Pathway (481); EPHA2 Forward Signaling Pathway (137961); PDGFR-beta Signaling Pathway (138071); Riboflavin Metabolism Pathway (83012); Riboflavin Metabolism Pathway (398)

ncbi summary :

The product of this gene belongs to the phosphotyrosine protein phosphatase family of proteins. It functions as an acid phosphatase and a protein tyrosine phosphatase by hydrolyzing protein tyrosine phosphate to protein tyrosine and orthophosphate. This enzyme also hydrolyzes orthophosphoric monoesters to alcohol and orthophosphate. This gene is genetically polymorphic, and three common alleles segregating at the corresponding locus give rise to six phenotypes. Each allele appears to encode at least two electrophoretically different isozymes, Bf and Bs, which are produced in allele-specific ratios. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Aug 2008]

uniprot summary :

ACP1: a phosphotyrosine protein phosphatase. Functions as an acid phosphatase and a protein tyrosine phosphatase by hydrolyzing protein tyrosine phosphate to protein tyrosine and orthophosphate. Hydrolyzes orthophosphoric monoesters to alcohol and orthophosphate. Genetically polymorphic with three common alleles segregating at the corresponding locus give rise to six phenotypes. Each allele appears to encode at least two electrophoretically different isozymes, Bf and Bs, which are produced in allele-specific ratios. Three transcript variants encoding distinct isoforms have been identified for this gene. Protein type: EC 3.1.3.48; Protein phosphatase, tyrosine (non-receptor); Motility/polarity/chemotaxis; Phosphatase (non-protein); Cofactor and Vitamin Metabolism - riboflavin; EC 3.1.3.2. Chromosomal Location of Human Ortholog: 2p25. Cellular Component: internal side of plasma membrane; cytoplasm. Molecular Function: acid phosphatase activity; protein binding; non-membrane spanning protein tyrosine phosphatase activity

size1 :

0.1 mg

price1 :

375 USD

size2 :

0.5 mg

price2 :

925

more info or order :

MyBioSource product webpage