GSTM1, 1-218aa, Mouse, Recombinant, E Coli | MyBioSource MBS203265 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

GSTM1, 1-218aa, Mouse, Recombinant, E Coli

catalog :

MBS203265

quantity :

0.1 mg

price :

255 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203265

products type :

Recombinant Protein

products full name :

GSTM1, 1-218aa, Mouse, Recombinant, E Coli

products short name :

GSTM1

products name syn :

GST class-mu1; GST 1-1; pmGT10; Glutathione S-transferase Mu 1 Glutathione S transferase mu; GST class Mu; GST class mu 1; GST mu; GST1; GSTM1.

other names :

glutathione S-transferase Mu 1; Glutathione S-transferase Mu 1; glutathione S-transferase Mu 1; GST 1-1; GST class-mu 1; glutathione S-transferase GT8.7; glutathione-S-transferase, mu 1; pmGT10; glutathione S-transferase, mu 1; GST 1-1; GST class-mu 1; Glutathione S-transferase GT8.7; pmGT10

products gene name :

GSTM1

other gene names :

Gstm1; Gstm1; Gstb1; Gstb-1

uniprot entry name :

GSTM1_MOUSE

sequence length :

218

sequence :

MPMILGYWNV RGLTHPIRML LEYTDSSYDE KRYTMGDAPD FDRSQWLNEK FKLGLDFPNL PYLIDGSHKI TQSNAILRYL ARKHHLDGET EEERIRADIV ENQVMDTRMQ LIMLCYNPDF EKQKPEFLKT IPEKMKLYSE FLGKRPWFAG DKVTYVDFLA YDILDQYRMF EPKCLDAFPN LRDFLARFEG LKKISAYMKS SRYIATPIFS KMAHWSNK

purity :

> 95% by SDS - PAGE

form :

Liquid. In PBS (pH7.4) containing 5mM glutathione

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Mouse

other info2 :

Expression System: E Coli. Bioactivity: Specific activity is 3 units/mg, and is defined as the amount of enzyme that conjugate 1.0 umole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25C. Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method). Assay: 1. Prepare a 1ml reaction mix into a suitable container: The final concentrations are 97 mM potassium phosphate, 0.97 mM EDTA, 2.5 mM glutathione, reduced, 1.0 mM 1-chloro-2,4-dinitrobenzene (CDNB), 3.2% (v/v) ethanol. 2. Equilibrate to 25C and monitor at A340nm until the value is constant using a spectrophotometer. 3. Add 50ul of recombinant mouse GSTM1 protein with various concentrations (1ug, 2ug, 5ug) in 950ul reaction buffer. 4. Mix by inversion and record the increase at A340nm for 5 minutes.

products categories :

Enzymes & Proteases

products description :

GSTM1 is a glutathione S-transferase that belongs to the mu class. This enzyme acts by catalyzing the reaction of glutathione with an acceptor molecule to form an S-substituted glutathione (S=sulfur). The reactions utilizing glutathione contribute the transformation of a wide variety of electrophiles, including reactive products of lipid, protein, carcinogens, therapeutic drugs, environmental toxins, and products of oxidative stress. Recombinant GSTM1 protein was expressed in E.coli and purified by using conventional chromatography techniques.

products references :

Lee KA., et al. (2001) Blood. 98(12), 3483-5. Hayes JD., et al. (1995) Crit Rev Biochem Mol Biol. 30(6):445-600.

ncbi gi num :

6754084

ncbi acc num :

NP_034488

ncbi gb acc num :

NM_010358.5

ncbi mol weight :

25.9 kDa (218 aa), confirmed by MALDI-TOF. (Molecular weight on SDS-PAGE will appear higher)

ncbi pathways :

Aflatoxin B1 Metabolism Pathway (198417); Biological Oxidations Pathway (1111015); Chemical Carcinogenesis Pathway (673229); Chemical Carcinogenesis Pathway (673237); Defective AHCY Causes Hypermethioninemia With S-adenosylhomocysteine Hydrolase Deficiency (HMAHCHD) Pathway (1111461); Defective GCLC Causes Hemolytic Anemia Due To Gamma-glutamylcysteine Synthetase Deficiency (HAGGSD) Pathway (1111480); Defective GGT1 Causes Glutathionuria (GLUTH) Pathway (1111481); Defective GSS Causes Glutathione Synthetase Deficiency (GSS Deficiency) Pathway (1111482); Defective MAT1A Causes Methionine Adenosyltransferase Deficiency (MATD) Pathway (1111485); Defective OPLAH Causes 5-oxoprolinase Deficiency (OPLAHD) Pathway (1111483)

uniprot summary :

GSTM1: Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Belongs to the GST superfamily. Mu family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Other Amino Acids Metabolism - glutathione; EC 2.5.1.18; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Xenobiotic Metabolism - metabolism by cytochrome P450; Transferase. Cellular Component: protein complex; cytoplasm; extracellular region; myelin sheath. Molecular Function: transferase activity; protein binding; protein homodimerization activity; glutathione transferase activity; protein heterodimerization activity; steroid binding; nickel ion binding. Biological Process: glutathione metabolic process; metabolic process

size1 :

0.1 mg

price1 :

255 USD

size2 :

0.5 mg

price2 :

535

more info or order :

MyBioSource product webpage