product summary
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company name :
MyBioSource
product type :
protein
product name :
G6PD,1-491aa, E Coli, Recombinant, E Coli
catalog :
MBS203264
quantity :
0.1 mg
price :
255 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS203264
products type :
Recombinant Protein
products full name :
G6PD,1-491aa, E Coli, Recombinant, E Coli
products short name :
G6PD
products name syn :
Glucose-6-phosphate 1-dehydrogenase; zwf; Glucose-6-phosphate 1-dehydrogenase G6PD; G6PD1; G6pdx; Glucose 6 phosphate 1 dehydrogenase; MET19; POS10; Zwf1p.
other names :
glucose-6-phosphate 1-dehydrogenase; Glucose-6-phosphate 1-dehydrogenase; glucose-6-phosphate 1-dehydrogenase
products gene name :
G6PD
other gene names :
zwf; zwf; ECK1853; JW1841; ; EDF
uniprot entry name :
G6PD_ECOLI
sequence length :
491
sequence :
MAVTQTAQAC DLVIFGAKGD LARRKLLPSL YQLEKAGQLN PDTRIIGVGR ADWDKAAYTK VVREALETFM KETIDEGLWD TLSARLDFCN LDVNDTAAFS RLGAMLDQKN RITINYFAMP PSTFGAICKG LGEAKLNAKP ARVVMEKPLG TSLATSQEIN DQVGEYFEEC QVYRIDHYLG KETVLNLLAL RFANSLFVNN WDNRTIDHVE ITVAEEVGIE GRWGYFDKAG QMRDMIQNHL LQILCMIAMS PPSDLSADSI RDEKVKVLKS LRRIDRSNVR EKTVRGQYTA GFAQGKKVPG YLEEEGANKS SNTETFVAIR VDIDNWRWAG VPFYLRTGKR LPTKCSEVVV YFKTPELNLF KESWQDLPQN KLTIRLQPDE GVDIQVLNKV PGLDHKHNLQ ITKLDLSYSE TFNQTHLADA YERLLLETMR GIQALFVRRD EVEEAWKWVD SITEAWAMDN DAPKPYQAGT WGPVASVAMI TRDGRSWNEF E
purity :
> 90% by SDS - PAGE
form :
In 50 mM MES 6.0, 0.1 mM PMSF, 2 mM EDTA, 0.5 mM DTT, 10% glycerol
concentration :
1 mg/ml (determined by Bradford assay)
storage stability :
Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
tested application :
SDS-PAGE
other info1 :
Antigen Species: E Coli
other info2 :
Expression System: E Coli. Bioactivity: Specific activity is 8-10 units/ml obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NAD or NADP. One unit oxidizes 1.0 umole D-glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of beta-NADP at pH 7.4 at 25C. Assay: 1. Prepare a 3ml reaction cocktail into a suitable container: The final concentrations are 50mM gylcylglycine, 2mM D-glucose 6-phosphate, 0.67mM beta-NADP, 10mM MgCl2. 2. Equilibrate to 25C and monitor at A340nm until the value is constant using a spectrophotometer. 3. Add 4ug of recombinant G6PD into reaction cocktail and mix immediately. 4. Record the increase at A340nm for 5 minutes.
products categories :
Enzymes & Proteases
products description :
Glucose-6-phosphate dehydrogenase (G6PD) is the rate-limiting enzyme of the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells by maintaining the level of NADPH. G6PD converts glucose-6-phosphate into 6-phosphoglucono-delta-lactone and simultaneously produce NADPH. The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. G6PD deficiency cause acute hemolytic anemia. Recombinant G6PD protein was expressed in E.coli and purified by conventional chromatography techniques.
products references :
Huang Y., et al. (2008) Mol Genet Metab. 93(1):44-53. Zimny A., et al. (2003) Pol Arch Med Wewn. 110(5):1327-33.
ncbi gi num :
16129805
ncbi acc num :
NP_416366
ncbi gb acc num :
NC_000913.3
uniprot acc num :
P0AC53
ncbi mol weight :
55.7kDa (491 aa), confirmed by MALDI-TOF.
ncbi pathways :
Biosynthesis Of Secondary Metabolites Pathway (148665); Carbon Metabolism Pathway (815459); Carbon Metabolism Pathway (817567); Entner-Doudoroff Pathway, Glucose-6P = Glyceraldehyde-3P + Pyruvate (405169); Entner-Doudoroff Pathway, Glucose-6P = Glyceraldehyde-3P + Pyruvate (468201); Glutathione Metabolism Pathway (1050); Glutathione Metabolism Pathway (343); Metabolic Pathways (131985); Microbial Metabolism In Diverse Environments Pathway (179780); Pentose Phosphate Pathway (1009)
ncbi summary :
Residues 199-203 of zwf encode a NNWDN peptide involved in ChpA(MazF) toxicity that is likely converted to NNWNN by AsnA; a zwf deletion prevents NNWNN activity (Kolodkin-Gal, 2007). [More information is available at EcoGene: EG11221]. A null mutation in the zwf gene encoding glucose 6-phosphate-1-dehydrogenase does not affect the growth rate significantly. [More information is available at EcoCyc: EG11221].
size1 :
0.1 mg
price1 :
255 USD
size2 :
0.5 mg
price2 :
535
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
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