COMT, 51-271aa, Human, Recombinant, E Coli | MyBioSource MBS203262 product information

product summary

company name :

MyBioSource

product type :

protein

product name :

COMT, 51-271aa, Human, Recombinant, E Coli

catalog :

MBS203262

quantity :

0.1 mg

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS203262

products type :

Recombinant Protein

products full name :

COMT, 51-271aa, Human, Recombinant, E Coli

products short name :

COMT

products name syn :

Catechol-O-methyltransferase; Catechol-O-methyltransferase; COMT

other names :

catechol O-methyltransferase isoform MB-COMT; Catechol O-methyltransferase; catechol O-methyltransferase; epididymis secretory sperm binding protein Li 98n; catechol-O-methyltransferase

products gene name :

COMT

other gene names :

COMT; COMT; HEL-S-98n

uniprot entry name :

COMT_HUMAN

sequence length :

271

sequence :

MGDTKEQRIL NHVLQHAEPG NAQSVLEAID TYCEQKEWAM NVGDKKGKIV DAVIQEHQPS VLLELGAYCG YSAVRMARLL SPGARLITIE INPDCAAITQ RMVDFAGVKD KVTLVVGASQ DIIPQLKKKY DVDTLDMVFL DHWKDRYLPD TLLLEECGLL RKGTVLLADN VICPGAPDFL AHVRGSSCFE CTHYQSFLEY REVVDGLEKA IYKGPGSEAG P

purity :

> 95% by SDS - PAGE

form :

Liquid. In 20 mM Tris-HCl buffer (pH 8.0) containing 1 mM MgCl2, 10% glycerol

concentration :

1 mg/ml (determined by Bradford assay)

storage stability :

Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

tested application :

SDS-PAGE

other info1 :

Antigen Species: Human

other info2 :

Expression System: E Coli. Endotoxin: < 1.0 Eu per 1 microgram of protein (determined by LAL method)

products categories :

Enzymes & Proteases

products description :

Catechol-O-methyltrasferase (COMT) is an enzyme that catalyses the transfer of the methyl group of S-adenosylmethionine (SAM) into catechol substrates such as levodopa, dopamine, epinephrine, and norepinephirine in the presence of Mg2+. COMT appears to be located in the postsynaptic neuron and plays roles in the metabolism of catechol estrogens and the inactivation of catecholamine neurotransmitters though enzymatic degradation. COMT inhibitors prevent levodopa degradation, increase its availability and are used in the treatment of patients with Parkinson's disease. Recombinant human COMT protein was expressed in E.coli and purified by using conventional chromatography techniques.

products references :

Cotton NJ., et al. (2004) J Biol Chem. 279(22):23710-8. Dawling S., et al. (2001) Cancer Res. 61(18):6716-22.

ncbi gi num :

4502969

ncbi acc num :

NP_000745

ncbi gb acc num :

NM_000754.3

ncbi mol weight :

24.4 kDa (221 aa), confirmed by MALDI-TOF.

ncbi pathways :

Biogenic Amine Synthesis Pathway (198793); Biological Oxidations Pathway (105698); Defective AHCY Causes Hypermethioninemia With S-adenosylhomocysteine Hydrolase Deficiency (HMAHCHD) Pathway (1127639); Defective GCLC Causes Hemolytic Anemia Due To Gamma-glutamylcysteine Synthetase Deficiency (HAGGSD) Pathway (1127658); Defective GGT1 Causes Glutathionuria (GLUTH) Pathway (1127659); Defective GSS Causes Glutathione Synthetase Deficiency (GSS Deficiency) Pathway (1127660); Defective MAT1A Causes Methionine Adenosyltransferase Deficiency (MATD) Pathway (1127663); Defective OPLAH Causes 5-oxoprolinase Deficiency (OPLAHD) Pathway (1127661); Defective SLC35D1 Causes Schneckenbecken Dysplasia (SCHBCKD) Pathway (1127665); Defective TPMT Causes Thiopurine S-methyltransferase Deficiency (TPMT Deficiency) Pathway (1127666)

ncbi summary :

Catechol-O-methyltransferase catalyzes the transfer of a methyl group from S-adenosylmethionine to catecholamines, including the neurotransmitters dopamine, epinephrine, and norepinephrine. This O-methylation results in one of the major degradative pathways of the catecholamine transmitters. In addition to its role in the metabolism of endogenous substances, COMT is important in the metabolism of catechol drugs used in the treatment of hypertension, asthma, and Parkinson disease. COMT is found in two forms in tissues, a soluble form (S-COMT) and a membrane-bound form (MB-COMT). The differences between S-COMT and MB-COMT reside within the N-termini. Several transcript variants are formed through the use of alternative translation initiation sites and promoters. [provided by RefSeq, Sep 2008]

uniprot summary :

COMT: Catalyzes the O-methylation, and thereby the inactivation, of catecholamine neurotransmitters and catechol hormones. Also shortens the biological half-lives of certain neuroactive drugs, like L-DOPA, alpha-methyl DOPA and isoproterenol. Belongs to the mammalian catechol-O-methyltransferase family. 2 isoforms of the human protein are produced by alternative initiation. Protein type: Amino Acid Metabolism - tyrosine; Methyltransferase; EC 2.1.1.6; Membrane protein, integral. Chromosomal Location of Human Ortholog: 22q11.21. Cellular Component: postsynaptic membrane; membrane; mitochondrion; axon; dendritic spine; plasma membrane; integral to membrane; cytosol. Molecular Function: protein binding; magnesium ion binding; O-methyltransferase activity; catechol O-methyltransferase activity. Biological Process: response to drug; methylation; estrogen metabolic process; cellular response to phosphate starvation; neurotransmitter catabolic process; dopamine catabolic process; negative regulation of smooth muscle cell proliferation; short-term memory; response to pain; response to lipopolysaccharide; female pregnancy; learning; reproductive process in a multicellular organism; positive regulation of homocysteine metabolic process; response to organic cyclic substance; negative regulation of dopamine metabolic process; synaptic transmission; xenobiotic metabolic process; regulation of sensory perception of pain; developmental process; neurotransmitter biosynthetic process. Disease: Schizophrenia; Panic Disorder 1

size1 :

0.1 mg

price1 :

510 USD

size2 :

0.5 mg

price2 :

1335

more info or order :

MyBioSource product webpage